Sympathetic reflexes after depletion of bulbospinal catecholaminergic neurons with anti-DβH-saporin

We examined the effects of destroying bulbospinal catecholaminergic neurons with the immunotoxin anti-dopamine β-hydroxylase-saporin (anti-DβH-Sap) on splanchnic nerve activity (SNA) and selected sympathetic reflexes in rats. Anti-DβH-Sap was administered into the thoracic spinal cord with the retrograde tracer fast blue. After 3-5 wk, anti-DβH-Sap eliminated most bulbospinal C1 (>74%), C3 (~84%), A5 (~98%), and A6 cells. Noncatecholaminergic bulbospinal neurons of the rostral ventrolateral medulla and serotonergic neurons were spared. Under chloralose anesthesia, mean arterial pressure and heart rate of anti-DβH-Sap-treated rats (3-5 wk) were normal. Resting SNA was not detectably altered, but the baroreflex range and gain were reduced ~40% (P < 0.05). Phenyl biguanide-induced decreases in mean arterial pressure, heart rate, and SNA were unchanged by anti-DβH-Sap, but the sympathoexcitatory response to intravenous cyanide was virtually abolished (P < 0.05). Rats that received spinal injections of saporin conjugated to an anti-mouse IgG had intact bulbospinal C1 and A5 cells and normal physiological responses. These data suggest that C1 and A5 neurons contribute modestly to resting SNA and cardiopulmonary reflexes. However, bulbospinal catecholaminergic neurons appear to play a prominent sympathoexcitatory role during stimulation of chemoreceptors.