Swiss population data on three tetrameric short tandem repeat loci - VWA, HUMTH01, and F13A1 -derived using multiplex PCR and laser fluorescence detection

M. N. Hochmeister, J. M. Jung, B. Budowle, U. V. Borer, R. Dirnhofer

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Allele and genotype frequencies for 3 tetrameric short tandem repeat loci VWA, HUMTHO1, and F13A1 were determined in a Swiss population sample using multiplex PCR and subsequent electrophoresis in DNA sequencing gels processed by automated laser fluorescence detection. The technique allows single base pair resolution and rapid typing, with a concomitant reduction in the potential for human transcriptional typing errors. All loci meet Hardy-Weinberg expectations. In addition, there is little evidence for association of alleles among the 3 loci. The allelic frequency data can be used in forensic analyses and paternity tests to estimate the frequency of a multiple STR locus DNA profile in the Swiss population.

Original languageEnglish
Pages (from-to)34-36
Number of pages3
JournalInternational journal of legal medicine
Volume107
Issue number1
DOIs
StatePublished - 1 Jan 1994

Keywords

  • Fluorescence detection
  • HUMTHO1 F13A1
  • Hardy-Weinberg expectations
  • Multiplex
  • PCR
  • Short tandem repeat loci
  • VWA

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