Swiss population data and forensic efficiency values on 3 tetrameric short tandem repeat loci-HUMTH01, TPOX, and CSFIPO - derived using a STR multiplex system

M. N. Hochmeister, B. Budowle, J. W. Schumm, C. J. Sprecher, U. V. Borer, R. Dirnhofer

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

Allele and genotype frequencies for 3 tetrameric short tandem repeat loci were determined in a Swiss population sample (n = 100) using the GenePrint STR Multiplex System, electrophoresis of the PCR products in DNA sequencing gels and subsequent detection of allelic fragments by silver staining. The loci are HUMTH01, TPOX, and CSFIPO. The observed heterozygosities are 83.0%, 60.0%, and 72.0%, respectively. The discrimination power determined for the individual loci is 0.914, 0.780, and 0.860, respectively, and the combined discrimination power for the triplex is 0.997. All loci meet Hardy-Weinberg expectations and after Bonferroni correction there was no evidence that the population sample deviates from expectations of independence. Moreover, independence of alleles at these STR loci with other PCR-based loci derived from the same Swiss population sample, previously reported, were considered. These loci were DQA1, LDLR, GYPA, HBGG, D7S8, GC and D1S80. Again, after Bonferroni correction there was no evidence that the population sample deviates from expectations of independence among alleles at the 10 different PCR-based loci. Thus, the allelic frequency data can be used in human identity testing to estimate the frequency of a multiple PCR-based DNA profile in the Swiss population.

Original languageEnglish
Pages (from-to)246-249
Number of pages4
JournalInternational journal of legal medicine
Volume107
Issue number5
DOIs
StatePublished - 1 Sep 1995

Keywords

  • CSFIPO-Polymarker
  • DNA-STR's
  • HUMTH01
  • Hardy-Weinberg expectations
  • Linkage equilibrium
  • PCR
  • Population genetics
  • Switzerland
  • TPOX

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