@article{4440c0b27a2b4aa28d6f88100dc640d2,
title = "Survival of aging CD264+ and CD264− populations of human bone marrow mesenchymal stem cells is independent of colony-forming efficiency",
abstract = "In vivo mesenchymal stem cell (MSC) survival is relevant to therapeutic applications requiring engraftment and potentially to nonengraftment applications as well. MSCs are a mixture of progenitors at different stages of cellular aging, but the contribution of this heterogeneity to the survival of MSC implants is unknown. Here, we employ a biomarker of cellular aging, the decoy TRAIL receptor CD264, to compare the survival kinetics of two cell populations in human bone marrow MSC (hBM-MSC) cultures. Sorted CD264+ hBM-MSCs from two age-matched donors have elevated β-galactosidase activity, decreased differentiation potential and form in vitro colonies inefficiently relative to CD264− hBM-MSCs. Counterintuitive to their aging phenotype, CD264+ hBM-MSCs exhibited comparable survival to matched CD264− hBM-MSCs from the same culture during in vitro colony formation and in vivo when implanted ectopically in immunodeficient NIH III mice. In vitro and in vivo survival of these two cell populations were independent of colony-forming efficiency. These findings have ramifications for the preparation of hBM-MSC therapies given the prevalence of aging CD264+ cells in hBM-MSC cultures and the popularity of colony-forming efficiency as a quality control metric in preclinical and clinical studies with MSCs.",
keywords = "aging, decoy TRAIL receptor 2 (CD264), mesenchymal stem cells, survival",
author = "Madsen, {Sean D.} and Jones, {Sean H.} and Tucker, {H. Alan} and Giler, {Margaret K.} and Muller, {Dyllan C.} and Discher, {Carson T.} and Russell, {Katie C.} and Dobek, {Georgina L.} and Sammarco, {Mimi C.} and Bunnell, {Bruce A.} and O'Connor, {Kim C.}",
note = "Funding Information: We are grateful for technical assistance from the Histology Core and Flow Cytometry Core in the Center for Stem Cell Research and Regenerative Medicine at Tulane University, Flow Cytometry Core at the Louisiana Cancer Research Consortium, and Microscopy and Imaging Core at Louisiana State University Health Sciences Center. This study was funded by the National Science Foundation (CBET-1604129, K. C. O.), National Institutes of Health (P51OD011104, B. A. B.), Tulane University Department of Comparative Medicine, Residency Research Program (K. C. O.), and Tulane University Carol Lavin Bernick Faculty Grant Program (K. C. O.). Any opinions expressed in this article are those of the authors and do not necessarily reflect the views of the National Science Foundation, National Institutes of Health or Tulane University. Funding Information: We are grateful for technical assistance from the Histology Core and Flow Cytometry Core in the Center for Stem Cell Research and Regenerative Medicine at Tulane University, Flow Cytometry Core at the Louisiana Cancer Research Consortium, and Microscopy and Imaging Core at Louisiana State University Health Sciences Center. This study was funded by the National Science Foundation (CBET‐1604129, K. C. O.), National Institutes of Health (P51OD011104, B. A. B.), Tulane University Department of Comparative Medicine, Residency Research Program (K. C. O.), and Tulane University Carol Lavin Bernick Faculty Grant Program (K. C. O.). Any opinions expressed in this article are those of the authors and do not necessarily reflect the views of the National Science Foundation, National Institutes of Health or Tulane University. Publisher Copyright: {\textcopyright} 2019 Wiley Periodicals, Inc.",
year = "2020",
month = jan,
day = "1",
doi = "10.1002/bit.27195",
language = "English",
volume = "117",
pages = "223--237",
journal = "Biotechnology and Bioengineering",
issn = "0006-3592",
publisher = "Wiley-VCH Verlag",
number = "1",
}