Surface plasmon-coupled polarized emission of N-acetyl-L-tryptophanamide

Ignacy Gryczynski; Joanna Malicka; Joanna Lukomska; Zygmunt Gryczynski; Joseph R. Lakowicz

doi:10.1562/2004-08-13-RA-273.1

Surface plasmon-coupled polarized emission of N-acetyl-L-tryptophanamide

Ignacy Gryczynski, Joanna Malicka, Joanna Lukomska, Zygmunt Gryczynski, Joseph R. Lakowicz

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Abstract

We report an observation of ultraviolet (UV) surface plasmon-coupled emission (SPCE) of N-acetyl-L-tryptophanamide (NATA). The sample was spin coated from poly(vinyl alcohol) (PVA) solution on 20 nm aluminum film deposited on a quartz substrate. The directional UV SPCE occurs within a well-defined narrow angle at 52° from the normal to the coupling hemicylinder quartz prism. The NATA directional emission is highly p polarized as expected for surface plasmon-coupled radiation. The 10 nm protective SiO₂ layer deposited on top of the aluminum film significantly neutralized the fluorophore quenching by the metal surface. SPCE of NATA demonstrates a remarkable intrinsic dispersive property-the maximum of the emission spectrum depends on the observation angle. The efficient spectral resolution of SPCE can be used in the construction of miniaturized spectrofluorometers. The observation of SPCE of tryptophan opens a new possibility for the study of many unlabeled proteins with the technique complementary to surface plasmon resonance analysis.

Original language	English
Pages (from-to)	482-485
Number of pages	4
Journal	Photochemistry and Photobiology
Volume	80
Issue number	3
DOIs	https://doi.org/10.1562/2004-08-13-RA-273.1
State	Published - Nov 2004

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title = "Surface plasmon-coupled polarized emission of N-acetyl-L-tryptophanamide",

abstract = "We report an observation of ultraviolet (UV) surface plasmon-coupled emission (SPCE) of N-acetyl-L-tryptophanamide (NATA). The sample was spin coated from poly(vinyl alcohol) (PVA) solution on 20 nm aluminum film deposited on a quartz substrate. The directional UV SPCE occurs within a well-defined narrow angle at 52° from the normal to the coupling hemicylinder quartz prism. The NATA directional emission is highly p polarized as expected for surface plasmon-coupled radiation. The 10 nm protective SiO2 layer deposited on top of the aluminum film significantly neutralized the fluorophore quenching by the metal surface. SPCE of NATA demonstrates a remarkable intrinsic dispersive property-the maximum of the emission spectrum depends on the observation angle. The efficient spectral resolution of SPCE can be used in the construction of miniaturized spectrofluorometers. The observation of SPCE of tryptophan opens a new possibility for the study of many unlabeled proteins with the technique complementary to surface plasmon resonance analysis.",

author = "Ignacy Gryczynski and Joanna Malicka and Joanna Lukomska and Zygmunt Gryczynski and Lakowicz, {Joseph R.}",

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T1 - Surface plasmon-coupled polarized emission of N-acetyl-L-tryptophanamide

AU - Gryczynski, Ignacy

AU - Malicka, Joanna

AU - Lukomska, Joanna

AU - Gryczynski, Zygmunt

AU - Lakowicz, Joseph R.

PY - 2004/11

Y1 - 2004/11

N2 - We report an observation of ultraviolet (UV) surface plasmon-coupled emission (SPCE) of N-acetyl-L-tryptophanamide (NATA). The sample was spin coated from poly(vinyl alcohol) (PVA) solution on 20 nm aluminum film deposited on a quartz substrate. The directional UV SPCE occurs within a well-defined narrow angle at 52° from the normal to the coupling hemicylinder quartz prism. The NATA directional emission is highly p polarized as expected for surface plasmon-coupled radiation. The 10 nm protective SiO2 layer deposited on top of the aluminum film significantly neutralized the fluorophore quenching by the metal surface. SPCE of NATA demonstrates a remarkable intrinsic dispersive property-the maximum of the emission spectrum depends on the observation angle. The efficient spectral resolution of SPCE can be used in the construction of miniaturized spectrofluorometers. The observation of SPCE of tryptophan opens a new possibility for the study of many unlabeled proteins with the technique complementary to surface plasmon resonance analysis.

AB - We report an observation of ultraviolet (UV) surface plasmon-coupled emission (SPCE) of N-acetyl-L-tryptophanamide (NATA). The sample was spin coated from poly(vinyl alcohol) (PVA) solution on 20 nm aluminum film deposited on a quartz substrate. The directional UV SPCE occurs within a well-defined narrow angle at 52° from the normal to the coupling hemicylinder quartz prism. The NATA directional emission is highly p polarized as expected for surface plasmon-coupled radiation. The 10 nm protective SiO2 layer deposited on top of the aluminum film significantly neutralized the fluorophore quenching by the metal surface. SPCE of NATA demonstrates a remarkable intrinsic dispersive property-the maximum of the emission spectrum depends on the observation angle. The efficient spectral resolution of SPCE can be used in the construction of miniaturized spectrofluorometers. The observation of SPCE of tryptophan opens a new possibility for the study of many unlabeled proteins with the technique complementary to surface plasmon resonance analysis.

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SN - 0031-8655

VL - 80

SP - 482

EP - 485

JO - Photochemistry and Photobiology

JF - Photochemistry and Photobiology

IS - 3

ER -

Surface plasmon-coupled polarized emission of N-acetyl-L-tryptophanamide

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