Surface plasmon-coupled polarized emission of N-acetyl-L-tryptophanamide

Ignacy Gryczynski, Joanna Malicka, Joanna Lukomska, Zygmunt Gryczynski, Joseph R. Lakowicz

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We report an observation of ultraviolet (UV) surface plasmon-coupled emission (SPCE) of N-acetyl-L-tryptophanamide (NATA). The sample was spin coated from poly(vinyl alcohol) (PVA) solution on 20 nm aluminum film deposited on a quartz substrate. The directional UV SPCE occurs within a well-defined narrow angle at 52° from the normal to the coupling hemicylinder quartz prism. The NATA directional emission is highly p polarized as expected for surface plasmon-coupled radiation. The 10 nm protective SiO2 layer deposited on top of the aluminum film significantly neutralized the fluorophore quenching by the metal surface. SPCE of NATA demonstrates a remarkable intrinsic dispersive property-the maximum of the emission spectrum depends on the observation angle. The efficient spectral resolution of SPCE can be used in the construction of miniaturized spectrofluorometers. The observation of SPCE of tryptophan opens a new possibility for the study of many unlabeled proteins with the technique complementary to surface plasmon resonance analysis.

Original languageEnglish
Pages (from-to)482-485
Number of pages4
JournalPhotochemistry and Photobiology
Volume80
Issue number3
DOIs
StatePublished - 1 Nov 2004

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Quartz
Observation
Aluminum
Surface Plasmon Resonance
Tryptophan
quartz
Metals
Alcohols
Radiation
aluminum
tryptophan
Fluorophores
surface plasmon resonance
Spectral resolution
spectral resolution
Surface plasmon resonance
prisms
metal surfaces
Prisms
N-acetyltryptophanamide

Cite this

Gryczynski, Ignacy ; Malicka, Joanna ; Lukomska, Joanna ; Gryczynski, Zygmunt ; Lakowicz, Joseph R. / Surface plasmon-coupled polarized emission of N-acetyl-L-tryptophanamide. In: Photochemistry and Photobiology. 2004 ; Vol. 80, No. 3. pp. 482-485.
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Surface plasmon-coupled polarized emission of N-acetyl-L-tryptophanamide. / Gryczynski, Ignacy; Malicka, Joanna; Lukomska, Joanna; Gryczynski, Zygmunt; Lakowicz, Joseph R.

In: Photochemistry and Photobiology, Vol. 80, No. 3, 01.11.2004, p. 482-485.

Research output: Contribution to journalArticle

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AU - Lakowicz, Joseph R.

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