Surface plasmon assisted microscopy: Reverse kretschmann fluorescence analysis of kinetics of hypertrophic cardiomyopathy heart

Julian Borejdo, P. Mettikolla, N. Calander, R. Luchowski, Ignacy Gryczynski, Zygmunt Gryczynski

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

It is believed that the alteration of the kinetics of interaction between actin and myosin causes a serious heart disease called Familial Hypertrophic Cardiomyopathy (FHC) by making a heart pump blood inefficiently. To check this hypothesis in ex-vivo heart, we constructed Surface Plasmon Assisted Microscope (SPAM) and used it in a reverse Kretschmann (RK) configuration. In SPAM fluorescence is the result of nearfield coupling of fluorophores excited in the vicinity of the metal coated surface of a coverslip with the surface plasmons propagating in the metal. Surface plasmons decouple on opposite side of the metal film and emit in directional manner as a far-field ppolarized radiation. In RK-SPAM a sample is illuminated directly by the laser beam. During contraction of heart muscle a myosin cross-bridge imparts periodic force impulses to actin. The impulses were analyzed by RK-SPAM by Fluorescence Correlation Spectroscopy (FCS) of fluorescently labeled actin. The rate of changes of orientation were significantly faster in contracting cardiac myofibrils of transgenic (R58Q) mice than of wild type (WT). These results suggest a way to rapidly diagnose this disease.

Original languageEnglish
Title of host publicationPlasmons
Subtitle of host publicationTheory and Applications
PublisherNova Science Publishers, Inc.
Pages161-178
Number of pages18
ISBN (Print)9781617613067
StatePublished - 1 Feb 2011

Fingerprint

microscopy
fluorescence
kinetics
microscopes
myosins
plasmons
impulses
blood pumps
heart diseases
muscles
metal films
contraction
metal surfaces
mice
far fields
laser beams
causes
radiation
configurations
metals

Cite this

Borejdo, J., Mettikolla, P., Calander, N., Luchowski, R., Gryczynski, I., & Gryczynski, Z. (2011). Surface plasmon assisted microscopy: Reverse kretschmann fluorescence analysis of kinetics of hypertrophic cardiomyopathy heart. In Plasmons: Theory and Applications (pp. 161-178). Nova Science Publishers, Inc..
Borejdo, Julian ; Mettikolla, P. ; Calander, N. ; Luchowski, R. ; Gryczynski, Ignacy ; Gryczynski, Zygmunt. / Surface plasmon assisted microscopy : Reverse kretschmann fluorescence analysis of kinetics of hypertrophic cardiomyopathy heart. Plasmons: Theory and Applications. Nova Science Publishers, Inc., 2011. pp. 161-178
@inbook{bb350d4f3e554927a1da7e5c9555bffa,
title = "Surface plasmon assisted microscopy: Reverse kretschmann fluorescence analysis of kinetics of hypertrophic cardiomyopathy heart",
abstract = "It is believed that the alteration of the kinetics of interaction between actin and myosin causes a serious heart disease called Familial Hypertrophic Cardiomyopathy (FHC) by making a heart pump blood inefficiently. To check this hypothesis in ex-vivo heart, we constructed Surface Plasmon Assisted Microscope (SPAM) and used it in a reverse Kretschmann (RK) configuration. In SPAM fluorescence is the result of nearfield coupling of fluorophores excited in the vicinity of the metal coated surface of a coverslip with the surface plasmons propagating in the metal. Surface plasmons decouple on opposite side of the metal film and emit in directional manner as a far-field ppolarized radiation. In RK-SPAM a sample is illuminated directly by the laser beam. During contraction of heart muscle a myosin cross-bridge imparts periodic force impulses to actin. The impulses were analyzed by RK-SPAM by Fluorescence Correlation Spectroscopy (FCS) of fluorescently labeled actin. The rate of changes of orientation were significantly faster in contracting cardiac myofibrils of transgenic (R58Q) mice than of wild type (WT). These results suggest a way to rapidly diagnose this disease.",
author = "Julian Borejdo and P. Mettikolla and N. Calander and R. Luchowski and Ignacy Gryczynski and Zygmunt Gryczynski",
year = "2011",
month = "2",
day = "1",
language = "English",
isbn = "9781617613067",
pages = "161--178",
booktitle = "Plasmons",
publisher = "Nova Science Publishers, Inc.",

}

Borejdo, J, Mettikolla, P, Calander, N, Luchowski, R, Gryczynski, I & Gryczynski, Z 2011, Surface plasmon assisted microscopy: Reverse kretschmann fluorescence analysis of kinetics of hypertrophic cardiomyopathy heart. in Plasmons: Theory and Applications. Nova Science Publishers, Inc., pp. 161-178.

Surface plasmon assisted microscopy : Reverse kretschmann fluorescence analysis of kinetics of hypertrophic cardiomyopathy heart. / Borejdo, Julian; Mettikolla, P.; Calander, N.; Luchowski, R.; Gryczynski, Ignacy; Gryczynski, Zygmunt.

Plasmons: Theory and Applications. Nova Science Publishers, Inc., 2011. p. 161-178.

Research output: Chapter in Book/Report/Conference proceedingChapter

TY - CHAP

T1 - Surface plasmon assisted microscopy

T2 - Reverse kretschmann fluorescence analysis of kinetics of hypertrophic cardiomyopathy heart

AU - Borejdo, Julian

AU - Mettikolla, P.

AU - Calander, N.

AU - Luchowski, R.

AU - Gryczynski, Ignacy

AU - Gryczynski, Zygmunt

PY - 2011/2/1

Y1 - 2011/2/1

N2 - It is believed that the alteration of the kinetics of interaction between actin and myosin causes a serious heart disease called Familial Hypertrophic Cardiomyopathy (FHC) by making a heart pump blood inefficiently. To check this hypothesis in ex-vivo heart, we constructed Surface Plasmon Assisted Microscope (SPAM) and used it in a reverse Kretschmann (RK) configuration. In SPAM fluorescence is the result of nearfield coupling of fluorophores excited in the vicinity of the metal coated surface of a coverslip with the surface plasmons propagating in the metal. Surface plasmons decouple on opposite side of the metal film and emit in directional manner as a far-field ppolarized radiation. In RK-SPAM a sample is illuminated directly by the laser beam. During contraction of heart muscle a myosin cross-bridge imparts periodic force impulses to actin. The impulses were analyzed by RK-SPAM by Fluorescence Correlation Spectroscopy (FCS) of fluorescently labeled actin. The rate of changes of orientation were significantly faster in contracting cardiac myofibrils of transgenic (R58Q) mice than of wild type (WT). These results suggest a way to rapidly diagnose this disease.

AB - It is believed that the alteration of the kinetics of interaction between actin and myosin causes a serious heart disease called Familial Hypertrophic Cardiomyopathy (FHC) by making a heart pump blood inefficiently. To check this hypothesis in ex-vivo heart, we constructed Surface Plasmon Assisted Microscope (SPAM) and used it in a reverse Kretschmann (RK) configuration. In SPAM fluorescence is the result of nearfield coupling of fluorophores excited in the vicinity of the metal coated surface of a coverslip with the surface plasmons propagating in the metal. Surface plasmons decouple on opposite side of the metal film and emit in directional manner as a far-field ppolarized radiation. In RK-SPAM a sample is illuminated directly by the laser beam. During contraction of heart muscle a myosin cross-bridge imparts periodic force impulses to actin. The impulses were analyzed by RK-SPAM by Fluorescence Correlation Spectroscopy (FCS) of fluorescently labeled actin. The rate of changes of orientation were significantly faster in contracting cardiac myofibrils of transgenic (R58Q) mice than of wild type (WT). These results suggest a way to rapidly diagnose this disease.

UR - http://www.scopus.com/inward/record.url?scp=84892133038&partnerID=8YFLogxK

M3 - Chapter

AN - SCOPUS:84892133038

SN - 9781617613067

SP - 161

EP - 178

BT - Plasmons

PB - Nova Science Publishers, Inc.

ER -

Borejdo J, Mettikolla P, Calander N, Luchowski R, Gryczynski I, Gryczynski Z. Surface plasmon assisted microscopy: Reverse kretschmann fluorescence analysis of kinetics of hypertrophic cardiomyopathy heart. In Plasmons: Theory and Applications. Nova Science Publishers, Inc. 2011. p. 161-178