Abstract
Colon cancer is one of the world’s most deadly diseases. Because of its internal location, it is necessary to obtain faster and more efficient diagnostic tools for this organ site. In this context, we studied the development of new luminescent nanoprobes (LNPs) as an alternative diagnostic apparatus for detecting this disease. The nanoparticles examined herein are lanthanide-doped sodium yttrium fluoride (NaYF 4 :Ln) and have shown to be promising as investigative devices. However, significant problems with the use of LNPs are the lack of biocompatibility and the targeting of the system to tumor regions. One of the strategies to bypass these problems is to increase of the particle lipophilicity modifying their surfaces with organic compounds that present high similarity to the biological system. In this work, we synthesized six new materials for use in bioimaging techniques obtained from the combination of nanoparticles of NaYF 4 :5%Eu with organic aromatic compounds covalently bonded. The materials were characterized structurally and morphologically using XRD and TEM, techniques, which showed the identification of the crystallographic phase β-NaYF 4 :5%Eu and its nanometric size (particles smaller than 50 nm). The conjugation process was confirmed by FT-IR spectra analysis and from the TGA profile. Excitation and emission spectra allowed the evaluation of the optical properties of the synthesized compounds. The interaction and cellular uptake was confirmed when HT-29 colon cancer cells were exposed to LNPs, indicating that the developed system has promising applications in bioimaging procedures. [Figure not available: see fulltext.].
| Original language | English |
|---|---|
| Article number | 23 |
| Journal | Journal of Nanoparticle Research |
| Volume | 21 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1 Jan 2019 |
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Keywords
- Cell imaging
- Couple reaction
- Diagnostic tool
- Luminescent nanoprobe
- NaYF :5%Eu
- Surface conjugation
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}
Surface modification strategy based on the conjugation of NaYF 4 :5%Eu luminescent nanoprobe with organic aromatic compounds for application in bioimaging assays . / da Silva Viana, Rodrigo; de Mascena Costa, Luciana Amaral; Leal, Andressa N.R.; Williams, Tyrslai M.; Luan, Liquian; Zhang, Guanyu; Wang, Maodie; Harmon, Ashlyn C.; dos Anjos, Janaina V.; Cueto, Rafael; Filho, Manoel Adrião Gomes; Falcão, Eduardo H.L.; Vicente, Maria G.H.; Junior, Severino A.; Mathis, James Michael.
In: Journal of Nanoparticle Research, Vol. 21, No. 1, 23, 01.01.2019.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Surface modification strategy based on the conjugation of NaYF 4 :5%Eu luminescent nanoprobe with organic aromatic compounds for application in bioimaging assays
AU - da Silva Viana, Rodrigo
AU - de Mascena Costa, Luciana Amaral
AU - Leal, Andressa N.R.
AU - Williams, Tyrslai M.
AU - Luan, Liquian
AU - Zhang, Guanyu
AU - Wang, Maodie
AU - Harmon, Ashlyn C.
AU - dos Anjos, Janaina V.
AU - Cueto, Rafael
AU - Filho, Manoel Adrião Gomes
AU - Falcão, Eduardo H.L.
AU - Vicente, Maria G.H.
AU - Junior, Severino A.
AU - Mathis, James Michael
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Colon cancer is one of the world’s most deadly diseases. Because of its internal location, it is necessary to obtain faster and more efficient diagnostic tools for this organ site. In this context, we studied the development of new luminescent nanoprobes (LNPs) as an alternative diagnostic apparatus for detecting this disease. The nanoparticles examined herein are lanthanide-doped sodium yttrium fluoride (NaYF 4 :Ln) and have shown to be promising as investigative devices. However, significant problems with the use of LNPs are the lack of biocompatibility and the targeting of the system to tumor regions. One of the strategies to bypass these problems is to increase of the particle lipophilicity modifying their surfaces with organic compounds that present high similarity to the biological system. In this work, we synthesized six new materials for use in bioimaging techniques obtained from the combination of nanoparticles of NaYF 4 :5%Eu with organic aromatic compounds covalently bonded. The materials were characterized structurally and morphologically using XRD and TEM, techniques, which showed the identification of the crystallographic phase β-NaYF 4 :5%Eu and its nanometric size (particles smaller than 50 nm). The conjugation process was confirmed by FT-IR spectra analysis and from the TGA profile. Excitation and emission spectra allowed the evaluation of the optical properties of the synthesized compounds. The interaction and cellular uptake was confirmed when HT-29 colon cancer cells were exposed to LNPs, indicating that the developed system has promising applications in bioimaging procedures. [Figure not available: see fulltext.].
AB - Colon cancer is one of the world’s most deadly diseases. Because of its internal location, it is necessary to obtain faster and more efficient diagnostic tools for this organ site. In this context, we studied the development of new luminescent nanoprobes (LNPs) as an alternative diagnostic apparatus for detecting this disease. The nanoparticles examined herein are lanthanide-doped sodium yttrium fluoride (NaYF 4 :Ln) and have shown to be promising as investigative devices. However, significant problems with the use of LNPs are the lack of biocompatibility and the targeting of the system to tumor regions. One of the strategies to bypass these problems is to increase of the particle lipophilicity modifying their surfaces with organic compounds that present high similarity to the biological system. In this work, we synthesized six new materials for use in bioimaging techniques obtained from the combination of nanoparticles of NaYF 4 :5%Eu with organic aromatic compounds covalently bonded. The materials were characterized structurally and morphologically using XRD and TEM, techniques, which showed the identification of the crystallographic phase β-NaYF 4 :5%Eu and its nanometric size (particles smaller than 50 nm). The conjugation process was confirmed by FT-IR spectra analysis and from the TGA profile. Excitation and emission spectra allowed the evaluation of the optical properties of the synthesized compounds. The interaction and cellular uptake was confirmed when HT-29 colon cancer cells were exposed to LNPs, indicating that the developed system has promising applications in bioimaging procedures. [Figure not available: see fulltext.].
KW - Cell imaging
KW - Couple reaction
KW - Diagnostic tool
KW - Luminescent nanoprobe
KW - NaYF :5%Eu
KW - Surface conjugation
UR - http://www.scopus.com/inward/record.url?scp=85060916728&partnerID=8YFLogxK
U2 - 10.1007/s11051-018-4422-0
DO - 10.1007/s11051-018-4422-0
M3 - Article
VL - 21
JO - Journal of Nanoparticle Research
JF - Journal of Nanoparticle Research
SN - 1388-0764
IS - 1
M1 - 23
ER -