TY - JOUR
T1 - [ 99 mTc-HYNIC-N-dodecylamide]:a new hydrophobic tracer for labelling reconstituted high-density lipoproteins (rHDL) for radioimaging
AU - Isaac-Olivé, Keila
AU - Ocampo-García, Blanca E.
AU - Aranda-Lara, Liliana
AU - Santos-Cuevas, Clara L.
AU - Jiménez-Mancilla, Nallely P.
AU - Luna-Gutiérrez, Myrna A.
AU - Medina, Luis A.
AU - Nagarajan, Bhavani
AU - Sabnis, Nirupama
AU - Raut, Sangram
AU - Prokai, Laszlo
AU - Lacko, Andras G.
N1 - Funding Information:
This work was supported by the Universidad Autónoma del Estado de México (UAEMex) and the University of North Texas Health Science Center (UNTHSC) through the joined “Seed-Grant” 3889/2015FS, Wheels for Wellness, Fort Worth, and the Robert A. Welch Foundation (endowment BK-0031 to L. P.). The authors thank Samantha M. Brewer and Professor Kayla N. Green (Texas Christian University, Fort Worth) for their help in the interpretation of the NMR spectra. This research was carried out as a part of the activities of the “Laboratorio Nacional de Investigación y Desarrollo de Radiofármacos”, CONACYT-Mexico and it was also supported by the Research Sectoral Fund for Education SEP-CONACYT-Mexico through the grant number CB-2016-287217. This work was supported in part by grants from Leukemia Texas and Texas Alzheimer’s Research Care and Consortium (2018-48-51-JI) to Sangram Raut.
Publisher Copyright:
© 2019 The Royal Society of Chemistry.
PY - 2019/1/14
Y1 - 2019/1/14
N2 - Despite the widespread use of nanotechnology in radio-imaging applications, lipoprotein based delivery systems have received only limited attention so far. These studies involve the synthesis of a novel hydrophobic radio-imaging tracer consisting of a hydrazinonicotinic acid (HYNIC)-N-dodecylamide and 99 mTc conjugate that can be encapsulated into rHDL nanoparticles (NPs). These rHDL NPs can selectively target the Scavenger Receptor type B1 (SR-B1) that is overexpressed on most cancer cells due to excess demand for cholesterol for membrane biogenesis and thus can target tumors in vivo. We provide details of the tracer synthesis, characterization of the rHDL/tracer complex, in vitro uptake, stability studies and in vivo application of this new radio-imaging approach.
AB - Despite the widespread use of nanotechnology in radio-imaging applications, lipoprotein based delivery systems have received only limited attention so far. These studies involve the synthesis of a novel hydrophobic radio-imaging tracer consisting of a hydrazinonicotinic acid (HYNIC)-N-dodecylamide and 99 mTc conjugate that can be encapsulated into rHDL nanoparticles (NPs). These rHDL NPs can selectively target the Scavenger Receptor type B1 (SR-B1) that is overexpressed on most cancer cells due to excess demand for cholesterol for membrane biogenesis and thus can target tumors in vivo. We provide details of the tracer synthesis, characterization of the rHDL/tracer complex, in vitro uptake, stability studies and in vivo application of this new radio-imaging approach.
UR - http://www.scopus.com/inward/record.url?scp=85059498774&partnerID=8YFLogxK
U2 - 10.1039/c8nr07484d
DO - 10.1039/c8nr07484d
M3 - Article
C2 - 30543234
AN - SCOPUS:85059498774
SN - 2040-3364
VL - 11
SP - 541
EP - 551
JO - Nanoscale
JF - Nanoscale
IS - 2
ER -