[ 99 mTc-HYNIC-N-dodecylamide]:a new hydrophobic tracer for labelling reconstituted high-density lipoproteins (rHDL) for radioimaging

Keila Isaac-Olivé, Blanca E. Ocampo-García, Liliana Aranda-Lara, Clara L. Santos-Cuevas, Nallely P. Jiménez-Mancilla, Myrna A. Luna-Gutiérrez, Luis A. Medina, Bhavani Nagarajan, Nirupama Sabnis, Sangram Limbaji Raut, Laszlo Prokai, Andras G. Lacko

Research output: Contribution to journalArticle

Abstract

Despite the widespread use of nanotechnology in radio-imaging applications, lipoprotein based delivery systems have received only limited attention so far. These studies involve the synthesis of a novel hydrophobic radio-imaging tracer consisting of a hydrazinonicotinic acid (HYNIC)-N-dodecylamide and 99 mTc conjugate that can be encapsulated into rHDL nanoparticles (NPs). These rHDL NPs can selectively target the Scavenger Receptor type B1 (SR-B1) that is overexpressed on most cancer cells due to excess demand for cholesterol for membrane biogenesis and thus can target tumors in vivo. We provide details of the tracer synthesis, characterization of the rHDL/tracer complex, in vitro uptake, stability studies and in vivo application of this new radio-imaging approach.

Original languageEnglish
Pages (from-to)541-551
Number of pages11
JournalNanoscale
Volume11
Issue number2
DOIs
StatePublished - 14 Jan 2019

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Lipoproteins
HDL Lipoproteins
Labeling
Imaging techniques
Acids
Nanoparticles
Scavenger Receptors
Nanotechnology
Tumors
Cholesterol
Cells
Membranes
hydrazinonicotinic acid

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Isaac-Olivé, K., Ocampo-García, B. E., Aranda-Lara, L., Santos-Cuevas, C. L., Jiménez-Mancilla, N. P., Luna-Gutiérrez, M. A., ... Lacko, A. G. (2019). [ 99 mTc-HYNIC-N-dodecylamide]:a new hydrophobic tracer for labelling reconstituted high-density lipoproteins (rHDL) for radioimaging Nanoscale, 11(2), 541-551. https://doi.org/10.1039/c8nr07484d
Isaac-Olivé, Keila ; Ocampo-García, Blanca E. ; Aranda-Lara, Liliana ; Santos-Cuevas, Clara L. ; Jiménez-Mancilla, Nallely P. ; Luna-Gutiérrez, Myrna A. ; Medina, Luis A. ; Nagarajan, Bhavani ; Sabnis, Nirupama ; Raut, Sangram Limbaji ; Prokai, Laszlo ; Lacko, Andras G. / [ 99 mTc-HYNIC-N-dodecylamide]:a new hydrophobic tracer for labelling reconstituted high-density lipoproteins (rHDL) for radioimaging In: Nanoscale. 2019 ; Vol. 11, No. 2. pp. 541-551.
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Isaac-Olivé, K, Ocampo-García, BE, Aranda-Lara, L, Santos-Cuevas, CL, Jiménez-Mancilla, NP, Luna-Gutiérrez, MA, Medina, LA, Nagarajan, B, Sabnis, N, Raut, SL, Prokai, L & Lacko, AG 2019, ' [ 99 mTc-HYNIC-N-dodecylamide]:a new hydrophobic tracer for labelling reconstituted high-density lipoproteins (rHDL) for radioimaging ', Nanoscale, vol. 11, no. 2, pp. 541-551. https://doi.org/10.1039/c8nr07484d

[ 99 mTc-HYNIC-N-dodecylamide]:a new hydrophobic tracer for labelling reconstituted high-density lipoproteins (rHDL) for radioimaging . / Isaac-Olivé, Keila; Ocampo-García, Blanca E.; Aranda-Lara, Liliana; Santos-Cuevas, Clara L.; Jiménez-Mancilla, Nallely P.; Luna-Gutiérrez, Myrna A.; Medina, Luis A.; Nagarajan, Bhavani; Sabnis, Nirupama; Raut, Sangram Limbaji; Prokai, Laszlo; Lacko, Andras G.

In: Nanoscale, Vol. 11, No. 2, 14.01.2019, p. 541-551.

Research output: Contribution to journalArticle

TY - JOUR

T1 - [ 99 mTc-HYNIC-N-dodecylamide]:a new hydrophobic tracer for labelling reconstituted high-density lipoproteins (rHDL) for radioimaging

AU - Isaac-Olivé, Keila

AU - Ocampo-García, Blanca E.

AU - Aranda-Lara, Liliana

AU - Santos-Cuevas, Clara L.

AU - Jiménez-Mancilla, Nallely P.

AU - Luna-Gutiérrez, Myrna A.

AU - Medina, Luis A.

AU - Nagarajan, Bhavani

AU - Sabnis, Nirupama

AU - Raut, Sangram Limbaji

AU - Prokai, Laszlo

AU - Lacko, Andras G.

PY - 2019/1/14

Y1 - 2019/1/14

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AB - Despite the widespread use of nanotechnology in radio-imaging applications, lipoprotein based delivery systems have received only limited attention so far. These studies involve the synthesis of a novel hydrophobic radio-imaging tracer consisting of a hydrazinonicotinic acid (HYNIC)-N-dodecylamide and 99 mTc conjugate that can be encapsulated into rHDL nanoparticles (NPs). These rHDL NPs can selectively target the Scavenger Receptor type B1 (SR-B1) that is overexpressed on most cancer cells due to excess demand for cholesterol for membrane biogenesis and thus can target tumors in vivo. We provide details of the tracer synthesis, characterization of the rHDL/tracer complex, in vitro uptake, stability studies and in vivo application of this new radio-imaging approach.

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VL - 11

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JO - Nanoscale

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Isaac-Olivé K, Ocampo-García BE, Aranda-Lara L, Santos-Cuevas CL, Jiménez-Mancilla NP, Luna-Gutiérrez MA et al. [ 99 mTc-HYNIC-N-dodecylamide]:a new hydrophobic tracer for labelling reconstituted high-density lipoproteins (rHDL) for radioimaging Nanoscale. 2019 Jan 14;11(2):541-551. https://doi.org/10.1039/c8nr07484d