Subtyping phosphoglucomutase-1 in semen stains and bloodstains: A report on the method

Bruce Budowle, R. S. Murch, L. C. Davidson, A. M. Gambel, J. J. Kearney

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A method is described for obtaining nondistorted, reproducible phosphoglucomutase-1 subtyping patterns from semen stains and bloodstains. Isoelectric focusing of phosphoglucomutase-1 was accomplished in 80 min in a 0.22-mm-thick polyacrylamide gel with an interelectrode wick distance of 8.0 cm. The gel contained 1.2% (w/v) N-(2-hydroxyethyl) piperazine-N-3-propanesulfonic acid (EPPS) and pH 5 to 7 ampholytes (4% w/v). When maintained at room temperature, laboratory-prepared bloodstains and semen stains could be typed for phosphoglucomutase-1 up to four months and three weeks, respectively. An evaluation of phosphoglucomutase-1 typing by isoelectric focusing and the Group I system was performed on casework samples submitted to the FBI Laboratory. In addition to the increased discriminating probability of phosphoglucomutase-1 when subtyped, isoelectric focusing yielded an increase in positive calls on questioned bloodstains (65.6 versus 36.2%) and dried seminal stains (16.4 versus 13.1%) compared with the Group I system.

Original languageEnglish
Pages (from-to)1341-1348
Number of pages8
JournalJournal of forensic sciences
Volume31
Issue number4
StatePublished - 1 Dec 1986

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Phosphoglucomutase
Semen
Coloring Agents
Isoelectric Focusing
casework
Group
Capillary Action
evaluation
Buffers
Gels
Temperature
Acids

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Budowle, B., Murch, R. S., Davidson, L. C., Gambel, A. M., & Kearney, J. J. (1986). Subtyping phosphoglucomutase-1 in semen stains and bloodstains: A report on the method. Journal of forensic sciences, 31(4), 1341-1348.
Budowle, Bruce ; Murch, R. S. ; Davidson, L. C. ; Gambel, A. M. ; Kearney, J. J. / Subtyping phosphoglucomutase-1 in semen stains and bloodstains : A report on the method. In: Journal of forensic sciences. 1986 ; Vol. 31, No. 4. pp. 1341-1348.
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Budowle, B, Murch, RS, Davidson, LC, Gambel, AM & Kearney, JJ 1986, 'Subtyping phosphoglucomutase-1 in semen stains and bloodstains: A report on the method', Journal of forensic sciences, vol. 31, no. 4, pp. 1341-1348.

Subtyping phosphoglucomutase-1 in semen stains and bloodstains : A report on the method. / Budowle, Bruce; Murch, R. S.; Davidson, L. C.; Gambel, A. M.; Kearney, J. J.

In: Journal of forensic sciences, Vol. 31, No. 4, 01.12.1986, p. 1341-1348.

Research output: Contribution to journalArticle

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AB - A method is described for obtaining nondistorted, reproducible phosphoglucomutase-1 subtyping patterns from semen stains and bloodstains. Isoelectric focusing of phosphoglucomutase-1 was accomplished in 80 min in a 0.22-mm-thick polyacrylamide gel with an interelectrode wick distance of 8.0 cm. The gel contained 1.2% (w/v) N-(2-hydroxyethyl) piperazine-N-3-propanesulfonic acid (EPPS) and pH 5 to 7 ampholytes (4% w/v). When maintained at room temperature, laboratory-prepared bloodstains and semen stains could be typed for phosphoglucomutase-1 up to four months and three weeks, respectively. An evaluation of phosphoglucomutase-1 typing by isoelectric focusing and the Group I system was performed on casework samples submitted to the FBI Laboratory. In addition to the increased discriminating probability of phosphoglucomutase-1 when subtyped, isoelectric focusing yielded an increase in positive calls on questioned bloodstains (65.6 versus 36.2%) and dried seminal stains (16.4 versus 13.1%) compared with the Group I system.

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