Subtyping group‐specific component or esterase D using the same ultrathin‐layer polyacrylamide gel format

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Abstract

An ultrathin‐layer polyacrylamide isoelectric focusing gel is described for obtaining highly resolved, nondistorted, reproducible patterns for group‐specific component (Gc) or esterase D (EsD) subtyping. The 1F, 1S, 2 and 1A1 bands of the Gc system were all typeable by isoelectric focusing alone. All common EsD allelic products were easily resolved. Typing Gc or EsD derived from bloodstains was also demonstrated.

Original languageEnglish
Pages (from-to)141-144
Number of pages4
JournalELECTROPHORESIS
Volume7
Issue number3
DOIs
StatePublished - 1 Jan 1986

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Isoelectric Focusing
Gels
s-formylglutathione hydrolase
polyacrylamide gels
polyacrylamide

Cite this

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abstract = "An ultrathin‐layer polyacrylamide isoelectric focusing gel is described for obtaining highly resolved, nondistorted, reproducible patterns for group‐specific component (Gc) or esterase D (EsD) subtyping. The 1F, 1S, 2 and 1A1 bands of the Gc system were all typeable by isoelectric focusing alone. All common EsD allelic products were easily resolved. Typing Gc or EsD derived from bloodstains was also demonstrated.",
author = "Bruce Budowle",
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Subtyping group‐specific component or esterase D using the same ultrathin‐layer polyacrylamide gel format. / Budowle, Bruce.

In: ELECTROPHORESIS, Vol. 7, No. 3, 01.01.1986, p. 141-144.

Research output: Contribution to journalArticle

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AB - An ultrathin‐layer polyacrylamide isoelectric focusing gel is described for obtaining highly resolved, nondistorted, reproducible patterns for group‐specific component (Gc) or esterase D (EsD) subtyping. The 1F, 1S, 2 and 1A1 bands of the Gc system were all typeable by isoelectric focusing alone. All common EsD allelic products were easily resolved. Typing Gc or EsD derived from bloodstains was also demonstrated.

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