Subarachnoid hemorrhage: Tests of association with apolipoprotein E and elastin genes

Ritesh Kaushal, Daniel Woo, Prodipto Pal, Mary Haverbusch, Huifeng Xi, Charles Moomaw, Padmini Sekar, Brett Kissela, Dawn Kleindorfer, Matthew Flaherty, Laura Sauerbeck, Ranajit Chakraborty, Joseph Broderick, Ranjan Deka

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Background: Apolipoprotein E (APOE) and elastin (ELN) are plausible candidate genes involved in the pathogenesis of stroke. We tested for association of variants in APOE and ELN with subarachnoid hemorrhage (SAH) in a population-based study. We genotyped 12 single nucleotide polymorphisms (SNPs) on APOE and 10 SNPs on ELN in a sample of 309 Caucasian individuals, of whom 107 are SAH cases and 202 are age-, race-, and gender-matched controls from the Greater Cincinnati/Northern Kentucky region. Associations were tested at genotype, allele, and haplotype levels. A genomic control analysis was performed to check for spurious associations resulting from population substructure. Results: At the APOE locus, no individual SNP was associated with SAH after correction for multiple comparisons. Haplotype analysis revealed significant association of the major haplotype (Hap1) in APOE with SAH (p = 0.001). The association stemmed from both the 5' promoter and the 3' region of the APOE gene. APOE ε2 and ε4 were not significantly associated with SAH. No association was observed for ELN at genotype, allele, or haplotype level and our study failed to confirm previous reports of ELN association with aneurysmal SAH. Conclusion: This study suggests a role of the APOE gene in the etiology of aneurysmal SAH.

Original languageEnglish
Article number49
JournalBMC Medical Genetics
Volume8
DOIs
StatePublished - 31 Jul 2007

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Elastin
Apolipoproteins E
Subarachnoid Hemorrhage
Haplotypes
Genes
Single Nucleotide Polymorphism
Alleles
Genotype
Apolipoprotein E2
Apolipoprotein E3
Apolipoprotein E4
Genetic Promoter Regions
Population
Stroke

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Kaushal, R., Woo, D., Pal, P., Haverbusch, M., Xi, H., Moomaw, C., ... Deka, R. (2007). Subarachnoid hemorrhage: Tests of association with apolipoprotein E and elastin genes. BMC Medical Genetics, 8, [49]. https://doi.org/10.1186/1471-2350-8-49
Kaushal, Ritesh ; Woo, Daniel ; Pal, Prodipto ; Haverbusch, Mary ; Xi, Huifeng ; Moomaw, Charles ; Sekar, Padmini ; Kissela, Brett ; Kleindorfer, Dawn ; Flaherty, Matthew ; Sauerbeck, Laura ; Chakraborty, Ranajit ; Broderick, Joseph ; Deka, Ranjan. / Subarachnoid hemorrhage : Tests of association with apolipoprotein E and elastin genes. In: BMC Medical Genetics. 2007 ; Vol. 8.
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abstract = "Background: Apolipoprotein E (APOE) and elastin (ELN) are plausible candidate genes involved in the pathogenesis of stroke. We tested for association of variants in APOE and ELN with subarachnoid hemorrhage (SAH) in a population-based study. We genotyped 12 single nucleotide polymorphisms (SNPs) on APOE and 10 SNPs on ELN in a sample of 309 Caucasian individuals, of whom 107 are SAH cases and 202 are age-, race-, and gender-matched controls from the Greater Cincinnati/Northern Kentucky region. Associations were tested at genotype, allele, and haplotype levels. A genomic control analysis was performed to check for spurious associations resulting from population substructure. Results: At the APOE locus, no individual SNP was associated with SAH after correction for multiple comparisons. Haplotype analysis revealed significant association of the major haplotype (Hap1) in APOE with SAH (p = 0.001). The association stemmed from both the 5' promoter and the 3' region of the APOE gene. APOE ε2 and ε4 were not significantly associated with SAH. No association was observed for ELN at genotype, allele, or haplotype level and our study failed to confirm previous reports of ELN association with aneurysmal SAH. Conclusion: This study suggests a role of the APOE gene in the etiology of aneurysmal SAH.",
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Kaushal, R, Woo, D, Pal, P, Haverbusch, M, Xi, H, Moomaw, C, Sekar, P, Kissela, B, Kleindorfer, D, Flaherty, M, Sauerbeck, L, Chakraborty, R, Broderick, J & Deka, R 2007, 'Subarachnoid hemorrhage: Tests of association with apolipoprotein E and elastin genes', BMC Medical Genetics, vol. 8, 49. https://doi.org/10.1186/1471-2350-8-49

Subarachnoid hemorrhage : Tests of association with apolipoprotein E and elastin genes. / Kaushal, Ritesh; Woo, Daniel; Pal, Prodipto; Haverbusch, Mary; Xi, Huifeng; Moomaw, Charles; Sekar, Padmini; Kissela, Brett; Kleindorfer, Dawn; Flaherty, Matthew; Sauerbeck, Laura; Chakraborty, Ranajit; Broderick, Joseph; Deka, Ranjan.

In: BMC Medical Genetics, Vol. 8, 49, 31.07.2007.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Subarachnoid hemorrhage

T2 - Tests of association with apolipoprotein E and elastin genes

AU - Kaushal, Ritesh

AU - Woo, Daniel

AU - Pal, Prodipto

AU - Haverbusch, Mary

AU - Xi, Huifeng

AU - Moomaw, Charles

AU - Sekar, Padmini

AU - Kissela, Brett

AU - Kleindorfer, Dawn

AU - Flaherty, Matthew

AU - Sauerbeck, Laura

AU - Chakraborty, Ranajit

AU - Broderick, Joseph

AU - Deka, Ranjan

PY - 2007/7/31

Y1 - 2007/7/31

N2 - Background: Apolipoprotein E (APOE) and elastin (ELN) are plausible candidate genes involved in the pathogenesis of stroke. We tested for association of variants in APOE and ELN with subarachnoid hemorrhage (SAH) in a population-based study. We genotyped 12 single nucleotide polymorphisms (SNPs) on APOE and 10 SNPs on ELN in a sample of 309 Caucasian individuals, of whom 107 are SAH cases and 202 are age-, race-, and gender-matched controls from the Greater Cincinnati/Northern Kentucky region. Associations were tested at genotype, allele, and haplotype levels. A genomic control analysis was performed to check for spurious associations resulting from population substructure. Results: At the APOE locus, no individual SNP was associated with SAH after correction for multiple comparisons. Haplotype analysis revealed significant association of the major haplotype (Hap1) in APOE with SAH (p = 0.001). The association stemmed from both the 5' promoter and the 3' region of the APOE gene. APOE ε2 and ε4 were not significantly associated with SAH. No association was observed for ELN at genotype, allele, or haplotype level and our study failed to confirm previous reports of ELN association with aneurysmal SAH. Conclusion: This study suggests a role of the APOE gene in the etiology of aneurysmal SAH.

AB - Background: Apolipoprotein E (APOE) and elastin (ELN) are plausible candidate genes involved in the pathogenesis of stroke. We tested for association of variants in APOE and ELN with subarachnoid hemorrhage (SAH) in a population-based study. We genotyped 12 single nucleotide polymorphisms (SNPs) on APOE and 10 SNPs on ELN in a sample of 309 Caucasian individuals, of whom 107 are SAH cases and 202 are age-, race-, and gender-matched controls from the Greater Cincinnati/Northern Kentucky region. Associations were tested at genotype, allele, and haplotype levels. A genomic control analysis was performed to check for spurious associations resulting from population substructure. Results: At the APOE locus, no individual SNP was associated with SAH after correction for multiple comparisons. Haplotype analysis revealed significant association of the major haplotype (Hap1) in APOE with SAH (p = 0.001). The association stemmed from both the 5' promoter and the 3' region of the APOE gene. APOE ε2 and ε4 were not significantly associated with SAH. No association was observed for ELN at genotype, allele, or haplotype level and our study failed to confirm previous reports of ELN association with aneurysmal SAH. Conclusion: This study suggests a role of the APOE gene in the etiology of aneurysmal SAH.

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U2 - 10.1186/1471-2350-8-49

DO - 10.1186/1471-2350-8-49

M3 - Article

C2 - 17672902

AN - SCOPUS:34548067702

VL - 8

JO - BMC Medical Genetics

JF - BMC Medical Genetics

SN - 1471-2350

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ER -