TY - JOUR
T1 - Structure of the neutral O-polysaccharide and biological activities of the lipopolysaccharide of Proteus mirabilis O20
AU - Kondakova, Anna N.
AU - Fudala, Rafal
AU - Bednarska, Katarzyna
AU - Senchenkova, Sof'Ya N.
AU - Knirel, Yuriy A.
AU - Kaca, Wieslaw
N1 - Funding Information:
This work was supported by grants from the Sciences Research Committee (KBN, Poland; 3P05A 098 24), from Swietokrzyska Academy, Russian Foundation for Basic Research (02-04-48767) and INTAS (YSF 2001/2-1).
PY - 2004/2/25
Y1 - 2004/2/25
N2 - Mild acid degradation of the lipopolysaccharide (LPS) of Proteus mirabilis O20 resulted in depolymerisation of the O-polysaccharide to give a repeating-unit pentasaccharide. A polysaccharide was obtained by O-deacylation of the LPS followed by nitrous acid deamination. The derived pentasaccharide and polysaccharide were studied by NMR spectroscopy, including 2D 1H,1H COSY, TOCSY, ROESY, 1H,13C HMQC and HMQC-TOSCY experiments, along with chemical methods, and the following structure of the repeating unit of the O-polysaccharide was established: (matrix presented). As opposite to most other P. mirabilis O-polysaccharides studied, that of P. mirabilis O20 is neutral. A week serological cross-reactivity was observed between anti-P. mirabilis O20 serum and LPS of a number of Proteus serogroups with known O-polysaccharide structure. The ability of LPS of P. mirabilis O20 to activate the serine protease cascade was tested in Limulus amoebocyte lysate and in human blood plasma and compared with that of P. mirabilis O14a,14c having an acidic O-polysaccharide. The LPS of P. mirabilis O20 was found to be less active in both assays than the LPS of P. mirabilis O14a,14c and, therefore, the structurally variable O-polysaccharide may influenced the biological activity of the conserved lipid A moiety of the LPS.
AB - Mild acid degradation of the lipopolysaccharide (LPS) of Proteus mirabilis O20 resulted in depolymerisation of the O-polysaccharide to give a repeating-unit pentasaccharide. A polysaccharide was obtained by O-deacylation of the LPS followed by nitrous acid deamination. The derived pentasaccharide and polysaccharide were studied by NMR spectroscopy, including 2D 1H,1H COSY, TOCSY, ROESY, 1H,13C HMQC and HMQC-TOSCY experiments, along with chemical methods, and the following structure of the repeating unit of the O-polysaccharide was established: (matrix presented). As opposite to most other P. mirabilis O-polysaccharides studied, that of P. mirabilis O20 is neutral. A week serological cross-reactivity was observed between anti-P. mirabilis O20 serum and LPS of a number of Proteus serogroups with known O-polysaccharide structure. The ability of LPS of P. mirabilis O20 to activate the serine protease cascade was tested in Limulus amoebocyte lysate and in human blood plasma and compared with that of P. mirabilis O14a,14c having an acidic O-polysaccharide. The LPS of P. mirabilis O20 was found to be less active in both assays than the LPS of P. mirabilis O14a,14c and, therefore, the structurally variable O-polysaccharide may influenced the biological activity of the conserved lipid A moiety of the LPS.
KW - Blood serum
KW - Lipopolysaccharide
KW - O-Polysaccharide structure
KW - Proteus mirabilis
KW - Serological cross-reactivity
UR - http://www.scopus.com/inward/record.url?scp=0842287581&partnerID=8YFLogxK
U2 - 10.1016/j.carres.2003.11.016
DO - 10.1016/j.carres.2003.11.016
M3 - Article
C2 - 15013399
AN - SCOPUS:0842287581
SN - 0008-6215
VL - 339
SP - 623
EP - 628
JO - Carbohydrate Research
JF - Carbohydrate Research
IS - 3
ER -