TY - JOUR
T1 - Structure of a lactic acid ether-containing and glycerol phosphate-containing O-polysaccharide from Proteus mirabilis O40
AU - Kondakova, Anna N.
AU - Fudala, Rafal
AU - Senchenkova, Sof'Ya N.
AU - Shashkov, Alexander S.
AU - Knirel, Yuriy A.
AU - Kaca, Wieslaw
N1 - Funding Information:
This work was supported by grants RF NSh-1557.2003.3, 02-04-48767 of the Russian Foundation for Basic Research, 4 TO9A 189 25 of the Sciences Research Committee (KBN, Poland) and Swietokrzyska Academy, Kielce, Poland.
PY - 2005/7/4
Y1 - 2005/7/4
N2 - An O-polysaccharide was isolated by mild acid hydrolysis from the lipopolysaccharide of Proteus mirabilis O40 and studied by NMR spectroscopy, including 2D 1H, 1H COSY, TOCSY, ROESY, and 1H, 13C HMQC experiments, along with chemical methods. The polysaccharide was found to contain an ether of GlcNAc with lactic acid and glycerol phosphate in the main chain and to have the following structure: → 3)-β-D-GlcpNAc4(R-Lac)-(1 → 3)-α-D-Galp-(1 → 3)-D-Gro-1-P-(O → 3)-β-D-GlcpNAc-(1 → where D-GlcpNAc4(R-Lac) stands for 2-acetamido-4-O-[(R)-1-carboxyethyl]-2-deoxy-D-glucose. This structure is unique among the known structures of the Proteus O-polysaccharides, which is in agreement with the classification of the strain studied into a separate O-serogroup. A serological relatedness of P. mirabilis O40 with some other Proteus strains was revealed and discussed in view of the O-polysaccharide structures.
AB - An O-polysaccharide was isolated by mild acid hydrolysis from the lipopolysaccharide of Proteus mirabilis O40 and studied by NMR spectroscopy, including 2D 1H, 1H COSY, TOCSY, ROESY, and 1H, 13C HMQC experiments, along with chemical methods. The polysaccharide was found to contain an ether of GlcNAc with lactic acid and glycerol phosphate in the main chain and to have the following structure: → 3)-β-D-GlcpNAc4(R-Lac)-(1 → 3)-α-D-Galp-(1 → 3)-D-Gro-1-P-(O → 3)-β-D-GlcpNAc-(1 → where D-GlcpNAc4(R-Lac) stands for 2-acetamido-4-O-[(R)-1-carboxyethyl]-2-deoxy-D-glucose. This structure is unique among the known structures of the Proteus O-polysaccharides, which is in agreement with the classification of the strain studied into a separate O-serogroup. A serological relatedness of P. mirabilis O40 with some other Proteus strains was revealed and discussed in view of the O-polysaccharide structures.
KW - Glycerol phosphate
KW - Lactic acid ether
KW - Lipopolysaccharide
KW - O-polysaccharide structure
KW - Proteus mirabilis
UR - http://www.scopus.com/inward/record.url?scp=20444391365&partnerID=8YFLogxK
U2 - 10.1016/j.carres.2005.04.002
DO - 10.1016/j.carres.2005.04.002
M3 - Article
C2 - 15890320
AN - SCOPUS:20444391365
VL - 340
SP - 1612
EP - 1617
JO - Carbohydrate Research
JF - Carbohydrate Research
SN - 0008-6215
IS - 9
ER -