Structure of a lactic acid ether-containing and glycerol phosphate-containing O-polysaccharide from Proteus mirabilis O40

Anna N. Kondakova, Rafal Fudala, Sof'Ya N. Senchenkova, Alexander S. Shashkov, Yuriy A. Knirel, Wieslaw Kaca

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

An O-polysaccharide was isolated by mild acid hydrolysis from the lipopolysaccharide of Proteus mirabilis O40 and studied by NMR spectroscopy, including 2D 1H, 1H COSY, TOCSY, ROESY, and 1H, 13C HMQC experiments, along with chemical methods. The polysaccharide was found to contain an ether of GlcNAc with lactic acid and glycerol phosphate in the main chain and to have the following structure: → 3)-β-D-GlcpNAc4(R-Lac)-(1 → 3)-α-D-Galp-(1 → 3)-D-Gro-1-P-(O → 3)-β-D-GlcpNAc-(1 → where D-GlcpNAc4(R-Lac) stands for 2-acetamido-4-O-[(R)-1-carboxyethyl]-2-deoxy-D-glucose. This structure is unique among the known structures of the Proteus O-polysaccharides, which is in agreement with the classification of the strain studied into a separate O-serogroup. A serological relatedness of P. mirabilis O40 with some other Proteus strains was revealed and discussed in view of the O-polysaccharide structures.

Original languageEnglish
Pages (from-to)1612-1617
Number of pages6
JournalCarbohydrate Research
Volume340
Issue number9
DOIs
StatePublished - 4 Jul 2005

Keywords

  • Glycerol phosphate
  • Lactic acid ether
  • Lipopolysaccharide
  • O-polysaccharide structure
  • Proteus mirabilis

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