Structure and serological characterization of the O-antigen of Proteus mirabilis O18 with a phosphocholine-containing oligosaccharide phosphate repeating unit

Rafal Fudala, Anna N. Kondakova, Katarzyna Bednarska, Sof'ya N. Senchenkova, Alexander S. Shashkov, Yuriy A. Knirel, Ulrich Zähringer, Wieslaw Kaca

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18 Scopus citations

Abstract

A phosphorylated, choline-containing polysaccharide was obtained by O-deacylation of the lipopolysaccharide (LPS) of Proteus mirabilis O18 by treatment with aqueous 12% ammonia, whereas hydrolysis with dilute acetic acid resulted in depolymerisation of the polysaccharide chain by the glycosyl phosphate linkage. Treatment of the O-deacylated LPS with aqueous 48% hydrofluoric acid cleaved the glycosyl phosphate group but, unexpectedly, did not affect the choline phosphate group. The polysaccharide and the derived oligosaccharides were studied by NMR spectroscopy, including 2D 1H,1H COSY, TOCSY, ROESY, 1H,13C HMQC and HMQC-TOSCY experiments, along with chemical methods, and the following structure of the pentasaccharide phosphate repeating unit was established:Where ChoP=Phosphocoline Immunochemical studies of the LPS, O-deacylated LPS and partially dephosphorylated pentasaccharide using rabbit polyclonal anti-P. mirabilis O18 serum showed the importance of the glycosyl phosphate group in manifesting the serological specificity of the O18-antigen.

Original languageEnglish
Pages (from-to)1835-1842
Number of pages8
JournalCarbohydrate Research
Volume338
Issue number18
DOIs
StatePublished - 1 Sep 2003

Keywords

  • Glycosyl phosphate
  • Lipopolysaccharide
  • O-Polysaccharide
  • Phosphocholine
  • Proteus mirabilis

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