Structural markers in prostate cancer serum imaged ex vivo using cryogenic transmission electron microscopy

Nazneen Aziz; Ashish K. Jha; Chris Thanos; Riyaz Basha; Arijit Bose

doi:10.1093/jmicro/dfq019

Structural markers in prostate cancer serum imaged ex vivo using cryogenic transmission electron microscopy

Nazneen Aziz, Ashish K. Jha, Chris Thanos, Riyaz Basha, Arijit Bose

Research output: Contribution to journal › Article › peer-review

Abstract

We used cryogenic transmission electron microscopy (cryo-TEM) to identify differences in macromolecular structures present in the serum from healthy individuals (HI) and prostate cancer (PCa) patients and show that these differences are potential markers for disease. Using a murine orthotopic model of human PCa, we determined that some of these structural markers in serum are associated with the tumour burden. These findings signify the potential of this nanoscale ex vivo imaging technology of body fluids as a platform for discovering early markers of PCa and other diseases.

Original language	English
Pages (from-to)	451-456
Number of pages	6
Journal	Journal of Electron Microscopy
Volume	59
Issue number	5
DOIs	https://doi.org/10.1093/jmicro/dfq019
State	Published - Oct 2010

Keywords

biomarkers
imaging
nanopathology
prostate cancer
secreted macromolecules
serum

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10.1093/jmicro/dfq019

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title = "Structural markers in prostate cancer serum imaged ex vivo using cryogenic transmission electron microscopy",

abstract = "We used cryogenic transmission electron microscopy (cryo-TEM) to identify differences in macromolecular structures present in the serum from healthy individuals (HI) and prostate cancer (PCa) patients and show that these differences are potential markers for disease. Using a murine orthotopic model of human PCa, we determined that some of these structural markers in serum are associated with the tumour burden. These findings signify the potential of this nanoscale ex vivo imaging technology of body fluids as a platform for discovering early markers of PCa and other diseases.",

keywords = "biomarkers, imaging, nanopathology, prostate cancer, secreted macromolecules, serum",

author = "Nazneen Aziz and Jha, {Ashish K.} and Chris Thanos and Riyaz Basha and Arijit Bose",

note = "Funding Information: The authors acknowledge the support from the Slater Technology Fund. We are grateful to Dr Peter Geck for generously providing serum samples of PCa and HI.",

year = "2010",

month = oct,

doi = "10.1093/jmicro/dfq019",

language = "English",

volume = "59",

pages = "451--456",

journal = "Journal of Electron Microscopy",

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T1 - Structural markers in prostate cancer serum imaged ex vivo using cryogenic transmission electron microscopy

AU - Aziz, Nazneen

AU - Jha, Ashish K.

AU - Thanos, Chris

AU - Basha, Riyaz

AU - Bose, Arijit

N1 - Funding Information: The authors acknowledge the support from the Slater Technology Fund. We are grateful to Dr Peter Geck for generously providing serum samples of PCa and HI.

PY - 2010/10

Y1 - 2010/10

N2 - We used cryogenic transmission electron microscopy (cryo-TEM) to identify differences in macromolecular structures present in the serum from healthy individuals (HI) and prostate cancer (PCa) patients and show that these differences are potential markers for disease. Using a murine orthotopic model of human PCa, we determined that some of these structural markers in serum are associated with the tumour burden. These findings signify the potential of this nanoscale ex vivo imaging technology of body fluids as a platform for discovering early markers of PCa and other diseases.

AB - We used cryogenic transmission electron microscopy (cryo-TEM) to identify differences in macromolecular structures present in the serum from healthy individuals (HI) and prostate cancer (PCa) patients and show that these differences are potential markers for disease. Using a murine orthotopic model of human PCa, we determined that some of these structural markers in serum are associated with the tumour burden. These findings signify the potential of this nanoscale ex vivo imaging technology of body fluids as a platform for discovering early markers of PCa and other diseases.

KW - biomarkers

KW - imaging

KW - nanopathology

KW - prostate cancer

KW - secreted macromolecules

KW - serum

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DO - 10.1093/jmicro/dfq019

M3 - Article

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AN - SCOPUS:77958002301

SN - 0022-0744

VL - 59

SP - 451

EP - 456

JO - Journal of Electron Microscopy

JF - Journal of Electron Microscopy

IS - 5

ER -

Structural markers in prostate cancer serum imaged ex vivo using cryogenic transmission electron microscopy

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