Structural and serological studies of the O-antigen of Proteus mirabilis O-9

Anna N. Kondakova; Rafal Fudala; Sof'ya N. Senchenkova; Aleksander S. Shashkov; Yuriy A. Knirel; Wieslaw Kaca

doi:10.1016/S0008-6215(03)00081-8

Structural and serological studies of the O-antigen of Proteus mirabilis O-9

Anna N. Kondakova, Rafal Fudala, Sof'ya N. Senchenkova, Aleksander S. Shashkov, Yuriy A. Knirel, Wieslaw Kaca

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

The following structure of the O-polysaccharide (O-antigen) of the lipopolysaccharide of Proteus mirabilis O-9 was determined by NMR spectroscopy, including 2D ¹H,¹H COSY, TOCSY, ROESY, and ¹H,¹³C HMQC experiments, along with chemical methods: matrix presented where the degree of O-acetylation is ∼70%. Immunochemical studies using rabbit polyclonal anti-Proteus mirabilis O-9 serum showed the importance of the O-acetyl groups in manifesting the serological specificity of the O-9 antigen. Anti-P. mirabilis O-9 cross-reacted with the lipopolysaccharides (LPS) of P. vulgaris O-25 and Proteus penneri 14, which could be accounted for by a structural similarity of their O-polysaccharides.

Original language	English
Pages (from-to)	1191-1196
Number of pages	6
Journal	Carbohydrate Research
Volume	338
Issue number	11
DOIs	https://doi.org/10.1016/S0008-6215(03)00081-8
State	Published - 23 May 2003

Keywords

Bacterial polysaccharide
Lipopolysaccharide
O-Acetylation
O-Antigen
Proteus mirabilis
Ribose
Structure

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@article{407f1835eaae4f16900657f8ef7d39de,

title = "Structural and serological studies of the O-antigen of Proteus mirabilis O-9",

abstract = "The following structure of the O-polysaccharide (O-antigen) of the lipopolysaccharide of Proteus mirabilis O-9 was determined by NMR spectroscopy, including 2D 1H,1H COSY, TOCSY, ROESY, and 1H,13C HMQC experiments, along with chemical methods: matrix presented where the degree of O-acetylation is ∼70%. Immunochemical studies using rabbit polyclonal anti-Proteus mirabilis O-9 serum showed the importance of the O-acetyl groups in manifesting the serological specificity of the O-9 antigen. Anti-P. mirabilis O-9 cross-reacted with the lipopolysaccharides (LPS) of P. vulgaris O-25 and Proteus penneri 14, which could be accounted for by a structural similarity of their O-polysaccharides.",

keywords = "Bacterial polysaccharide, Lipopolysaccharide, O-Acetylation, O-Antigen, Proteus mirabilis, Ribose, Structure",

author = "Kondakova, {Anna N.} and Rafal Fudala and Senchenkova, {Sof'ya N.} and Shashkov, {Aleksander S.} and Knirel, {Yuriy A.} and Wieslaw Kaca",

note = "Funding Information: This work was supported by of the Science Research Committee (KBN, Poland; grant 6P04C 05620), the University of Lodz, the Russian Foundation for Basic Research (grant 02-04-48767) and INTAS (grant YSF 2001/2-0001).",

year = "2003",

month = may,

day = "23",

doi = "10.1016/S0008-6215(03)00081-8",

language = "English",

volume = "338",

pages = "1191--1196",

journal = "Carbohydrate Research",

issn = "0008-6215",

publisher = "Elsevier BV",

number = "11",

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TY - JOUR

T1 - Structural and serological studies of the O-antigen of Proteus mirabilis O-9

AU - Kondakova, Anna N.

AU - Fudala, Rafal

AU - Senchenkova, Sof'ya N.

AU - Shashkov, Aleksander S.

AU - Knirel, Yuriy A.

AU - Kaca, Wieslaw

N1 - Funding Information: This work was supported by of the Science Research Committee (KBN, Poland; grant 6P04C 05620), the University of Lodz, the Russian Foundation for Basic Research (grant 02-04-48767) and INTAS (grant YSF 2001/2-0001).

PY - 2003/5/23

Y1 - 2003/5/23

N2 - The following structure of the O-polysaccharide (O-antigen) of the lipopolysaccharide of Proteus mirabilis O-9 was determined by NMR spectroscopy, including 2D 1H,1H COSY, TOCSY, ROESY, and 1H,13C HMQC experiments, along with chemical methods: matrix presented where the degree of O-acetylation is ∼70%. Immunochemical studies using rabbit polyclonal anti-Proteus mirabilis O-9 serum showed the importance of the O-acetyl groups in manifesting the serological specificity of the O-9 antigen. Anti-P. mirabilis O-9 cross-reacted with the lipopolysaccharides (LPS) of P. vulgaris O-25 and Proteus penneri 14, which could be accounted for by a structural similarity of their O-polysaccharides.

AB - The following structure of the O-polysaccharide (O-antigen) of the lipopolysaccharide of Proteus mirabilis O-9 was determined by NMR spectroscopy, including 2D 1H,1H COSY, TOCSY, ROESY, and 1H,13C HMQC experiments, along with chemical methods: matrix presented where the degree of O-acetylation is ∼70%. Immunochemical studies using rabbit polyclonal anti-Proteus mirabilis O-9 serum showed the importance of the O-acetyl groups in manifesting the serological specificity of the O-9 antigen. Anti-P. mirabilis O-9 cross-reacted with the lipopolysaccharides (LPS) of P. vulgaris O-25 and Proteus penneri 14, which could be accounted for by a structural similarity of their O-polysaccharides.

KW - Bacterial polysaccharide

KW - Lipopolysaccharide

KW - O-Acetylation

KW - O-Antigen

KW - Proteus mirabilis

KW - Ribose

KW - Structure

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U2 - 10.1016/S0008-6215(03)00081-8

DO - 10.1016/S0008-6215(03)00081-8

M3 - Article

C2 - 12747861

AN - SCOPUS:0038582675

SN - 0008-6215

VL - 338

SP - 1191

EP - 1196

JO - Carbohydrate Research

JF - Carbohydrate Research

IS - 11

ER -

Structural and serological studies of the O-antigen of Proteus mirabilis O-9

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