Abstract
The following structure of the O-polysaccharide (O-antigen) of the lipopolysaccharide of Proteus mirabilis O-9 was determined by NMR spectroscopy, including 2D 1H,1H COSY, TOCSY, ROESY, and 1H,13C HMQC experiments, along with chemical methods: matrix presented where the degree of O-acetylation is ∼70%. Immunochemical studies using rabbit polyclonal anti-Proteus mirabilis O-9 serum showed the importance of the O-acetyl groups in manifesting the serological specificity of the O-9 antigen. Anti-P. mirabilis O-9 cross-reacted with the lipopolysaccharides (LPS) of P. vulgaris O-25 and Proteus penneri 14, which could be accounted for by a structural similarity of their O-polysaccharides.
Original language | English |
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Pages (from-to) | 1191-1196 |
Number of pages | 6 |
Journal | Carbohydrate Research |
Volume | 338 |
Issue number | 11 |
DOIs | |
State | Published - 23 May 2003 |
Keywords
- Bacterial polysaccharide
- Lipopolysaccharide
- O-Acetylation
- O-Antigen
- Proteus mirabilis
- Ribose
- Structure