Structural and serological studies of the O-antigen of Proteus mirabilis O-9

Anna N. Kondakova, Rafal Fudala, Sof'ya N. Senchenkova, Aleksander S. Shashkov, Yuriy A. Knirel, Wieslaw Kaca

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14 Scopus citations

Abstract

The following structure of the O-polysaccharide (O-antigen) of the lipopolysaccharide of Proteus mirabilis O-9 was determined by NMR spectroscopy, including 2D 1H,1H COSY, TOCSY, ROESY, and 1H,13C HMQC experiments, along with chemical methods: matrix presented where the degree of O-acetylation is ∼70%. Immunochemical studies using rabbit polyclonal anti-Proteus mirabilis O-9 serum showed the importance of the O-acetyl groups in manifesting the serological specificity of the O-9 antigen. Anti-P. mirabilis O-9 cross-reacted with the lipopolysaccharides (LPS) of P. vulgaris O-25 and Proteus penneri 14, which could be accounted for by a structural similarity of their O-polysaccharides.

Original languageEnglish
Pages (from-to)1191-1196
Number of pages6
JournalCarbohydrate Research
Volume338
Issue number11
DOIs
StatePublished - 23 May 2003

Keywords

  • Bacterial polysaccharide
  • Lipopolysaccharide
  • O-Acetylation
  • O-Antigen
  • Proteus mirabilis
  • Ribose
  • Structure

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