Stress-induced changes in neuronal Aquaporin-9 (AQP9) in a retinal ganglion cell-line

Adnan Dibas, Ming Hui Yang, Joseph Bobich, Thomas Yorio

Research output: Contribution to journalArticleResearchpeer-review

27 Citations (Scopus)

Abstract

The water channel, Aquaporin-9 (AQP9) is enriched in selected neuronal populations and is unique its ability to act as a lactate-glycerol channel supplying neurons with alternative fuel under ischaemic conditions. AQP9 was detected in RGC-5 cells, a retinal ganglion cell-line, primary RGCs, and retina by Western blotting, real-time PCR (RT-PCR) and immunohistochemistry. RGC-5 cells subjected to a hypotonic stress increased their cell volume that was blocked by known inhibitor of AQP9 (phloretin (40 μM)). RGC-5 cells subjected to hypoxia, showed an up-regulation in AQP9 expression as judged by Western blotting and RT-PCR. Similarly, hypotonic shock (50%) increased AQP9 expression as determined by RT-PCR. AQP9 is involved in energy balance as a glycerol-lactate channel and also appears to regulate cell volume in retinal ganglion neurons. This water channel may play a key role in retinal ganglion pathology.

Original languageEnglish
Pages (from-to)378-384
Number of pages7
JournalPharmacological Research
Volume55
Issue number5
DOIs
StatePublished - 1 May 2007

Fingerprint

Aquaporins
Retinal Ganglion Cells
Cell Line
Real-Time Polymerase Chain Reaction
Osmotic Pressure
Cell Size
Ganglia
Glycerol
Lactic Acid
Western Blotting
Phloretin
Retinal Neurons
Retina
Up-Regulation
Immunohistochemistry
Pathology
Neurons

Keywords

  • Aquaporin-9
  • Ganglion
  • Hypotonic
  • Hypoxia
  • Optic-nerve
  • Retina

Cite this

Dibas, Adnan ; Yang, Ming Hui ; Bobich, Joseph ; Yorio, Thomas. / Stress-induced changes in neuronal Aquaporin-9 (AQP9) in a retinal ganglion cell-line. In: Pharmacological Research. 2007 ; Vol. 55, No. 5. pp. 378-384.
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Stress-induced changes in neuronal Aquaporin-9 (AQP9) in a retinal ganglion cell-line. / Dibas, Adnan; Yang, Ming Hui; Bobich, Joseph; Yorio, Thomas.

In: Pharmacological Research, Vol. 55, No. 5, 01.05.2007, p. 378-384.

Research output: Contribution to journalArticleResearchpeer-review

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