Strain and age effects on aqueous humor dynamics in the mouse

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

PURPOSE. We evaluated differences in aqueous humor dynamics (AHD) among several mouse strains within younger and older age groups. METHODS. Albino (A/J, BALB/cJ) and pigmented (C3H/HeJ, C57-BL/6J) mice (young [2½-4½ months] and aged [10-12 months]) were studied. Intraocular pressure (IOP) was measured. In cannulated eyes, episcleral venous pressure (Pe) was assessed (blood reflux). Other AHD parameters (outflow facility [C], aqueous humor formation rate [Fin]) were assessed (constant flow infusion). Uveoscleral outflow rate (Fu) was obtained by calculation (Fu(calc)) using the modified Goldmann equation, and in additional eyes (for comparison), by FITC-dextran perfusion (Fu(FITC-dex)). RESULTS. Intraocular pressure was higher in pigmented strains, but did not exhibit age- dependence, except in the C57-BL/6J strain. Fu(calc) decreased with age in BALB/cJ (↓83.3%), C3H/HeJ (↓78.0%), and C57-BL/6J (↓85.0%) strains. In the A/J strain, Fu(calc) decreased with age (↓70.0%), but not significantly. Fin decreased with age in the C3H/HeJ (↓53.6%) strain. In C57-BL/6J and A/J strains, Fin decreased with age, but not significantly. C in the BALB/cJ strain increased with age (↑62.5%). In C3H/HeJ and C57-BL/6J strains, C increased with age, but not significantly. Episcleral venous pressure ranged from 6.0 to 6.6 mm Hg (albino strains) to 8.5 to 8.9 mm Hg (pigmented strains). Pe was not age dependent, but was higher in pigmented animals. CONCLUSIONS. In mouse, Fu and Fin diminish with age. C tends to increase as animals progress to middle life. There are strain differences in Fu, IOP, C, Fin, and Pe. The current findings provide an important foundation for comparisons among different strains in different study reports.

Original languageEnglish
Pages (from-to)5764-5776
Number of pages13
JournalInvestigative Ophthalmology and Visual Science
Volume56
Issue number10
DOIs
StatePublished - 1 Jan 2015

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Aqueous Humor
Intraocular Pressure
Venous Pressure
Pressure
Fluorescein-5-isothiocyanate
Age Groups
Perfusion

Keywords

  • Age
  • Aqueous humor formation
  • Aqueous outflow facility
  • Episcleral venous pressure
  • Intraocular pressure
  • Mouse
  • Strain
  • Uveoscleral outflow

Cite this

@article{763163829181443d94b7cbd22b927471,
title = "Strain and age effects on aqueous humor dynamics in the mouse",
abstract = "PURPOSE. We evaluated differences in aqueous humor dynamics (AHD) among several mouse strains within younger and older age groups. METHODS. Albino (A/J, BALB/cJ) and pigmented (C3H/HeJ, C57-BL/6J) mice (young [2½-4½ months] and aged [10-12 months]) were studied. Intraocular pressure (IOP) was measured. In cannulated eyes, episcleral venous pressure (Pe) was assessed (blood reflux). Other AHD parameters (outflow facility [C], aqueous humor formation rate [Fin]) were assessed (constant flow infusion). Uveoscleral outflow rate (Fu) was obtained by calculation (Fu(calc)) using the modified Goldmann equation, and in additional eyes (for comparison), by FITC-dextran perfusion (Fu(FITC-dex)). RESULTS. Intraocular pressure was higher in pigmented strains, but did not exhibit age- dependence, except in the C57-BL/6J strain. Fu(calc) decreased with age in BALB/cJ (↓83.3{\%}), C3H/HeJ (↓78.0{\%}), and C57-BL/6J (↓85.0{\%}) strains. In the A/J strain, Fu(calc) decreased with age (↓70.0{\%}), but not significantly. Fin decreased with age in the C3H/HeJ (↓53.6{\%}) strain. In C57-BL/6J and A/J strains, Fin decreased with age, but not significantly. C in the BALB/cJ strain increased with age (↑62.5{\%}). In C3H/HeJ and C57-BL/6J strains, C increased with age, but not significantly. Episcleral venous pressure ranged from 6.0 to 6.6 mm Hg (albino strains) to 8.5 to 8.9 mm Hg (pigmented strains). Pe was not age dependent, but was higher in pigmented animals. CONCLUSIONS. In mouse, Fu and Fin diminish with age. C tends to increase as animals progress to middle life. There are strain differences in Fu, IOP, C, Fin, and Pe. The current findings provide an important foundation for comparisons among different strains in different study reports.",
keywords = "Age, Aqueous humor formation, Aqueous outflow facility, Episcleral venous pressure, Intraocular pressure, Mouse, Strain, Uveoscleral outflow",
author = "{Cameron Millar}, J. and Phan, {Tien N.} and Pang, {Iok Hou} and Clark, {Abbot F.}",
year = "2015",
month = "1",
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doi = "10.1167/iovs.15-16720",
language = "English",
volume = "56",
pages = "5764--5776",
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number = "10",

}

Strain and age effects on aqueous humor dynamics in the mouse. / Cameron Millar, J.; Phan, Tien N.; Pang, Iok Hou; Clark, Abbot F.

In: Investigative Ophthalmology and Visual Science, Vol. 56, No. 10, 01.01.2015, p. 5764-5776.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Strain and age effects on aqueous humor dynamics in the mouse

AU - Cameron Millar, J.

AU - Phan, Tien N.

AU - Pang, Iok Hou

AU - Clark, Abbot F.

PY - 2015/1/1

Y1 - 2015/1/1

N2 - PURPOSE. We evaluated differences in aqueous humor dynamics (AHD) among several mouse strains within younger and older age groups. METHODS. Albino (A/J, BALB/cJ) and pigmented (C3H/HeJ, C57-BL/6J) mice (young [2½-4½ months] and aged [10-12 months]) were studied. Intraocular pressure (IOP) was measured. In cannulated eyes, episcleral venous pressure (Pe) was assessed (blood reflux). Other AHD parameters (outflow facility [C], aqueous humor formation rate [Fin]) were assessed (constant flow infusion). Uveoscleral outflow rate (Fu) was obtained by calculation (Fu(calc)) using the modified Goldmann equation, and in additional eyes (for comparison), by FITC-dextran perfusion (Fu(FITC-dex)). RESULTS. Intraocular pressure was higher in pigmented strains, but did not exhibit age- dependence, except in the C57-BL/6J strain. Fu(calc) decreased with age in BALB/cJ (↓83.3%), C3H/HeJ (↓78.0%), and C57-BL/6J (↓85.0%) strains. In the A/J strain, Fu(calc) decreased with age (↓70.0%), but not significantly. Fin decreased with age in the C3H/HeJ (↓53.6%) strain. In C57-BL/6J and A/J strains, Fin decreased with age, but not significantly. C in the BALB/cJ strain increased with age (↑62.5%). In C3H/HeJ and C57-BL/6J strains, C increased with age, but not significantly. Episcleral venous pressure ranged from 6.0 to 6.6 mm Hg (albino strains) to 8.5 to 8.9 mm Hg (pigmented strains). Pe was not age dependent, but was higher in pigmented animals. CONCLUSIONS. In mouse, Fu and Fin diminish with age. C tends to increase as animals progress to middle life. There are strain differences in Fu, IOP, C, Fin, and Pe. The current findings provide an important foundation for comparisons among different strains in different study reports.

AB - PURPOSE. We evaluated differences in aqueous humor dynamics (AHD) among several mouse strains within younger and older age groups. METHODS. Albino (A/J, BALB/cJ) and pigmented (C3H/HeJ, C57-BL/6J) mice (young [2½-4½ months] and aged [10-12 months]) were studied. Intraocular pressure (IOP) was measured. In cannulated eyes, episcleral venous pressure (Pe) was assessed (blood reflux). Other AHD parameters (outflow facility [C], aqueous humor formation rate [Fin]) were assessed (constant flow infusion). Uveoscleral outflow rate (Fu) was obtained by calculation (Fu(calc)) using the modified Goldmann equation, and in additional eyes (for comparison), by FITC-dextran perfusion (Fu(FITC-dex)). RESULTS. Intraocular pressure was higher in pigmented strains, but did not exhibit age- dependence, except in the C57-BL/6J strain. Fu(calc) decreased with age in BALB/cJ (↓83.3%), C3H/HeJ (↓78.0%), and C57-BL/6J (↓85.0%) strains. In the A/J strain, Fu(calc) decreased with age (↓70.0%), but not significantly. Fin decreased with age in the C3H/HeJ (↓53.6%) strain. In C57-BL/6J and A/J strains, Fin decreased with age, but not significantly. C in the BALB/cJ strain increased with age (↑62.5%). In C3H/HeJ and C57-BL/6J strains, C increased with age, but not significantly. Episcleral venous pressure ranged from 6.0 to 6.6 mm Hg (albino strains) to 8.5 to 8.9 mm Hg (pigmented strains). Pe was not age dependent, but was higher in pigmented animals. CONCLUSIONS. In mouse, Fu and Fin diminish with age. C tends to increase as animals progress to middle life. There are strain differences in Fu, IOP, C, Fin, and Pe. The current findings provide an important foundation for comparisons among different strains in different study reports.

KW - Age

KW - Aqueous humor formation

KW - Aqueous outflow facility

KW - Episcleral venous pressure

KW - Intraocular pressure

KW - Mouse

KW - Strain

KW - Uveoscleral outflow

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U2 - 10.1167/iovs.15-16720

DO - 10.1167/iovs.15-16720

M3 - Article

C2 - 26325415

AN - SCOPUS:84940841173

VL - 56

SP - 5764

EP - 5776

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 10

ER -