STR primer concordance study

Bruce Budowle, Arni Masibay, Stacey J. Anderson, Charles Barna, Lisa Biega, Susanne Brenneke, Barry L. Brown, Jill Cramer, Gretchen A. DeGroot, Derek Douglas, Barry Duceman, Allison Eastman, Robert Giles, Jennifer Hamill, Daniel J. Haase, Dirk W. Janssen, Timothy D. Kupferschmid, Terry Lawton, Christine Lemire, Barbara LlewellynTamyra Moretti, Jennifer Neves, Chris Palaski, Sindey Schueler, Joanne Sgueglia, Cynthia Sprecher, Christine Tomsey, Don Yet

Research output: Contribution to journalArticle

82 Scopus citations

Abstract

Over 1500 population database samples comprising African Americans, Caucasians, Hispanics, Native Americans, Chamorros and Filipinos were typed using the PowerPlex® 16 and the Profiler Plus™/COfiler™ kits. Except for the D8S1179 locus in Chamorros and Filipinos from Guam, there were eight examples in which a typing difference due to allele dropout was observed. At the D8S1179 locus in the population samples from Guam, there were 13 examples of allele dropout observed when using the Profiler Plus kit. The data support that the primers used in the PowerPlex® 16, Profiler Plus™, and COfiler™ kits are reliable for typing reference samples that are for use in CODIS. In addition, allele frequency databases have been established for the STR loci Penta D and Penta E. Both loci are highly polymorphic.

Original languageEnglish
Pages (from-to)47-54
Number of pages8
JournalForensic Science International
Volume124
Issue number1
DOIs
StatePublished - 15 Dec 2001

Keywords

  • Allele dropout
  • AmpFlSTR COfiler™ kit
  • AmpFlSTR Profiler Plus™ kit
  • CODIS
  • Concordance
  • Hardy-Weinberg expectations
  • Penta D
  • Penta E
  • PowerPlex 16 kit
  • PowerPlex 2.1 kit
  • Primers
  • STR
  • Stochastic effects

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    Budowle, B., Masibay, A., Anderson, S. J., Barna, C., Biega, L., Brenneke, S., Brown, B. L., Cramer, J., DeGroot, G. A., Douglas, D., Duceman, B., Eastman, A., Giles, R., Hamill, J., Haase, D. J., Janssen, D. W., Kupferschmid, T. D., Lawton, T., Lemire, C., ... Yet, D. (2001). STR primer concordance study. Forensic Science International, 124(1), 47-54. https://doi.org/10.1016/S0379-0738(01)00563-1