Steady-state and time-resolved fluorescence studies of stripped Borage oil

Bogdan Smyk, Ryszard Amarowicz, Mariusz Szabelski, Ignacy Gryczynski, Zygmunt Gryczynski

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9 Scopus citations


In this study we explored the spectroscopic properties of Borage oil, particularly the use of fluorescence techniques to investigate the presence of conjugated fatty acids (CFAs). This research has important health and dietary applications. The absorption and fluorescence spectra of different CFAs and Borage oil in ethanol were measured. Time-domain fluorescence was employed to establish the life times of the samples. We found that Borage oil contains 1.2 × 10-3 mol L-1 of α-eleostearic acid or its isomer (i.e., a conjugated triene), 1.6 × 10-4 mol L-1 of cis-parinaric acid (i.e., a conjugated tetraene) and 1.1 × 10-5 mol L-1 of c-COPA (i.e., a conjugated pentaene). Because of the three-exponential fluorescence intensity decay for Borage oil, other fatty acids with a four conjugated double bond system could not be excluded.

Original languageEnglish
Pages (from-to)85-89
Number of pages5
JournalAnalytica Chimica Acta
Issue number1-2
StatePublished - 30 Jul 2009


  • Borage oil
  • Conjugated fatty acids (CFAs)
  • Pentaene
  • Steady-state fluorescence spectroscopy
  • Tetraene
  • Time-resolved fluorescence spectroscopy
  • Triene


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