Standard reference for instrument response function in fluorescence lifetime measurements in visible and near infrared

Rahul Chib, Sunil Shah, Zygmunt Gryczynski, Rafal Fudala, Julian Borejdo, Bogumil Zelent, Maria G. Corradini, Richard D. Ludescher, Ignacy Gryczynski

Research output: Contribution to journalArticle

2 Scopus citations


Allura red (AR) fluorophore, a common dye in the food industry, displays a broad emission spectrum in water (visible-to-near infrared region of the electromagnetic spectrum) and has a remarkably short fluorescence lifetime of about 10 ps. This short lifetime does not depend on the emission (observation) wavelength. We examined time responses of AR fluorescence across emission wavelengths from 550 nm to 750 nm and found that it is an ideal candidate for impulse response functions in fluorescence lifetime measurements.

Original languageEnglish
Article number027001
JournalMeasurement Science and Technology
Issue number2
StatePublished - 29 Dec 2015



  • allura red
  • fluorescence lifetime
  • instrument response function
  • lifetime standard
  • scatterer substitute

Cite this