Standard reference for instrument response function in fluorescence lifetime measurements in visible and near infrared

Rahul Chib, Sunil Shah, Zygmunt Gryczynski, Rafal Fudala, Julian Borejdo, Bogumil Zelent, Maria G. Corradini, Richard D. Ludescher, Ignacy Gryczynski

Research output: Contribution to journalArticleResearchpeer-review

2 Citations (Scopus)

Abstract

Allura red (AR) fluorophore, a common dye in the food industry, displays a broad emission spectrum in water (visible-to-near infrared region of the electromagnetic spectrum) and has a remarkably short fluorescence lifetime of about 10 ps. This short lifetime does not depend on the emission (observation) wavelength. We examined time responses of AR fluorescence across emission wavelengths from 550 nm to 750 nm and found that it is an ideal candidate for impulse response functions in fluorescence lifetime measurements.

Original languageEnglish
Article number027001
JournalMeasurement Science and Technology
Volume27
Issue number2
DOIs
StatePublished - 29 Dec 2015

Fingerprint

Fluorescence Lifetime
Response Function
Infrared
Fluorescence
Infrared radiation
life (durability)
fluorescence
Wavelength
Impulse Response Function
electromagnetic spectra
Fluorophores
time response
Dyes
Impulse response
food
wavelengths
Response Time
impulses
Lifetime
emission spectra

Keywords

  • allura red
  • fluorescence lifetime
  • instrument response function
  • lifetime standard
  • scatterer substitute

Cite this

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title = "Standard reference for instrument response function in fluorescence lifetime measurements in visible and near infrared",
abstract = "Allura red (AR) fluorophore, a common dye in the food industry, displays a broad emission spectrum in water (visible-to-near infrared region of the electromagnetic spectrum) and has a remarkably short fluorescence lifetime of about 10 ps. This short lifetime does not depend on the emission (observation) wavelength. We examined time responses of AR fluorescence across emission wavelengths from 550 nm to 750 nm and found that it is an ideal candidate for impulse response functions in fluorescence lifetime measurements.",
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author = "Rahul Chib and Sunil Shah and Zygmunt Gryczynski and Rafal Fudala and Julian Borejdo and Bogumil Zelent and Corradini, {Maria G.} and Ludescher, {Richard D.} and Ignacy Gryczynski",
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Standard reference for instrument response function in fluorescence lifetime measurements in visible and near infrared. / Chib, Rahul; Shah, Sunil; Gryczynski, Zygmunt; Fudala, Rafal; Borejdo, Julian; Zelent, Bogumil; Corradini, Maria G.; Ludescher, Richard D.; Gryczynski, Ignacy.

In: Measurement Science and Technology, Vol. 27, No. 2, 027001, 29.12.2015.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Standard reference for instrument response function in fluorescence lifetime measurements in visible and near infrared

AU - Chib, Rahul

AU - Shah, Sunil

AU - Gryczynski, Zygmunt

AU - Fudala, Rafal

AU - Borejdo, Julian

AU - Zelent, Bogumil

AU - Corradini, Maria G.

AU - Ludescher, Richard D.

AU - Gryczynski, Ignacy

PY - 2015/12/29

Y1 - 2015/12/29

N2 - Allura red (AR) fluorophore, a common dye in the food industry, displays a broad emission spectrum in water (visible-to-near infrared region of the electromagnetic spectrum) and has a remarkably short fluorescence lifetime of about 10 ps. This short lifetime does not depend on the emission (observation) wavelength. We examined time responses of AR fluorescence across emission wavelengths from 550 nm to 750 nm and found that it is an ideal candidate for impulse response functions in fluorescence lifetime measurements.

AB - Allura red (AR) fluorophore, a common dye in the food industry, displays a broad emission spectrum in water (visible-to-near infrared region of the electromagnetic spectrum) and has a remarkably short fluorescence lifetime of about 10 ps. This short lifetime does not depend on the emission (observation) wavelength. We examined time responses of AR fluorescence across emission wavelengths from 550 nm to 750 nm and found that it is an ideal candidate for impulse response functions in fluorescence lifetime measurements.

KW - allura red

KW - fluorescence lifetime

KW - instrument response function

KW - lifetime standard

KW - scatterer substitute

UR - http://www.scopus.com/inward/record.url?scp=84955511476&partnerID=8YFLogxK

U2 - 10.1088/0957-0233/27/2/027001

DO - 10.1088/0957-0233/27/2/027001

M3 - Article

VL - 27

JO - Measurement Science and Technology

JF - Measurement Science and Technology

SN - 0957-0233

IS - 2

M1 - 027001

ER -