Stability of isoniazid injection in i.v. solutions

Jun H. Lee, Brad Moriyama, Stacey A. Henning, Robert L. Danner, Thomas J. Walsh, Scott Robert Penzak, George J. Grimes, Gopal K. Potti

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Purpose. Results of an assessment of the chemical stability of isoniazid injection in 0.9% sodium chloride injection and 5% dextrose injection are reported. Methods. Triplicate solutions of isoniazid (0.5 and 6.0 mg/mL) in the 2 diluents were prepared in ethylene and propylene copolymer i.v. containers and stored under light protection at room temperature (20–25 °C) or under refrigeration (2–8 °C). Standard aliquots were removed from each solution at time points up to 72 hours and analyzed via high-performance liquid chromatography (HPLC). Stability was defined as retention of >90% of the initial isoniazid concentration; pH, osmolality, and visual appearance were assessed. Results. Isoniazid 0.5- and 6.0-mg/mL solutions in 0.9% sodium chloride injection were stable for up to 72 hours at room temperature or under refrigeration. HPLC analysis of isoniazid 0.5-mg/mL solutions in 5% dextrose injection revealed a decrease to less than 90% of the initial concentration at 8 hours at room temperature and at 30 hours under refrigeration. Isoniazid 6.0-mg/mL solutions in 5% dextrose injection were stable for 24 hours at room temperature and for 48 hours under refrigeration. The pH, osmolality, and visual appearance of the solutions were not affected. Conclusion. Isoniazid solutions of 0.5 and 6.0 mg/mL in 0.9% sodium chloride injection were stable under light protection for up to 72 hours when stored at room temperature or under refrigeration. Isoniazid injection was less stable in 5% dextrose injection, especially at a concentration of 0.5 mg/mL at room temperature.

Original languageEnglish
Pages (from-to)622-626
Number of pages5
JournalAmerican Journal of Health-System Pharmacy
Volume75
Issue number10
DOIs
StatePublished - 15 May 2018

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Isoniazid
Refrigeration
Injections
Temperature
Sodium Chloride
Glucose
Osmolar Concentration
High Pressure Liquid Chromatography
Light

Keywords

  • Intravenous
  • Isoniazid
  • Stability

Cite this

Lee, J. H., Moriyama, B., Henning, S. A., Danner, R. L., Walsh, T. J., Penzak, S. R., ... Potti, G. K. (2018). Stability of isoniazid injection in i.v. solutions. American Journal of Health-System Pharmacy, 75(10), 622-626. https://doi.org/10.2146/ajhp170268
Lee, Jun H. ; Moriyama, Brad ; Henning, Stacey A. ; Danner, Robert L. ; Walsh, Thomas J. ; Penzak, Scott Robert ; Grimes, George J. ; Potti, Gopal K. / Stability of isoniazid injection in i.v. solutions. In: American Journal of Health-System Pharmacy. 2018 ; Vol. 75, No. 10. pp. 622-626.
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title = "Stability of isoniazid injection in i.v. solutions",
abstract = "Purpose. Results of an assessment of the chemical stability of isoniazid injection in 0.9{\%} sodium chloride injection and 5{\%} dextrose injection are reported. Methods. Triplicate solutions of isoniazid (0.5 and 6.0 mg/mL) in the 2 diluents were prepared in ethylene and propylene copolymer i.v. containers and stored under light protection at room temperature (20–25 °C) or under refrigeration (2–8 °C). Standard aliquots were removed from each solution at time points up to 72 hours and analyzed via high-performance liquid chromatography (HPLC). Stability was defined as retention of >90{\%} of the initial isoniazid concentration; pH, osmolality, and visual appearance were assessed. Results. Isoniazid 0.5- and 6.0-mg/mL solutions in 0.9{\%} sodium chloride injection were stable for up to 72 hours at room temperature or under refrigeration. HPLC analysis of isoniazid 0.5-mg/mL solutions in 5{\%} dextrose injection revealed a decrease to less than 90{\%} of the initial concentration at 8 hours at room temperature and at 30 hours under refrigeration. Isoniazid 6.0-mg/mL solutions in 5{\%} dextrose injection were stable for 24 hours at room temperature and for 48 hours under refrigeration. The pH, osmolality, and visual appearance of the solutions were not affected. Conclusion. Isoniazid solutions of 0.5 and 6.0 mg/mL in 0.9{\%} sodium chloride injection were stable under light protection for up to 72 hours when stored at room temperature or under refrigeration. Isoniazid injection was less stable in 5{\%} dextrose injection, especially at a concentration of 0.5 mg/mL at room temperature.",
keywords = "Intravenous, Isoniazid, Stability",
author = "Lee, {Jun H.} and Brad Moriyama and Henning, {Stacey A.} and Danner, {Robert L.} and Walsh, {Thomas J.} and Penzak, {Scott Robert} and Grimes, {George J.} and Potti, {Gopal K.}",
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Lee, JH, Moriyama, B, Henning, SA, Danner, RL, Walsh, TJ, Penzak, SR, Grimes, GJ & Potti, GK 2018, 'Stability of isoniazid injection in i.v. solutions', American Journal of Health-System Pharmacy, vol. 75, no. 10, pp. 622-626. https://doi.org/10.2146/ajhp170268

Stability of isoniazid injection in i.v. solutions. / Lee, Jun H.; Moriyama, Brad; Henning, Stacey A.; Danner, Robert L.; Walsh, Thomas J.; Penzak, Scott Robert; Grimes, George J.; Potti, Gopal K.

In: American Journal of Health-System Pharmacy, Vol. 75, No. 10, 15.05.2018, p. 622-626.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Stability of isoniazid injection in i.v. solutions

AU - Lee, Jun H.

AU - Moriyama, Brad

AU - Henning, Stacey A.

AU - Danner, Robert L.

AU - Walsh, Thomas J.

AU - Penzak, Scott Robert

AU - Grimes, George J.

AU - Potti, Gopal K.

PY - 2018/5/15

Y1 - 2018/5/15

N2 - Purpose. Results of an assessment of the chemical stability of isoniazid injection in 0.9% sodium chloride injection and 5% dextrose injection are reported. Methods. Triplicate solutions of isoniazid (0.5 and 6.0 mg/mL) in the 2 diluents were prepared in ethylene and propylene copolymer i.v. containers and stored under light protection at room temperature (20–25 °C) or under refrigeration (2–8 °C). Standard aliquots were removed from each solution at time points up to 72 hours and analyzed via high-performance liquid chromatography (HPLC). Stability was defined as retention of >90% of the initial isoniazid concentration; pH, osmolality, and visual appearance were assessed. Results. Isoniazid 0.5- and 6.0-mg/mL solutions in 0.9% sodium chloride injection were stable for up to 72 hours at room temperature or under refrigeration. HPLC analysis of isoniazid 0.5-mg/mL solutions in 5% dextrose injection revealed a decrease to less than 90% of the initial concentration at 8 hours at room temperature and at 30 hours under refrigeration. Isoniazid 6.0-mg/mL solutions in 5% dextrose injection were stable for 24 hours at room temperature and for 48 hours under refrigeration. The pH, osmolality, and visual appearance of the solutions were not affected. Conclusion. Isoniazid solutions of 0.5 and 6.0 mg/mL in 0.9% sodium chloride injection were stable under light protection for up to 72 hours when stored at room temperature or under refrigeration. Isoniazid injection was less stable in 5% dextrose injection, especially at a concentration of 0.5 mg/mL at room temperature.

AB - Purpose. Results of an assessment of the chemical stability of isoniazid injection in 0.9% sodium chloride injection and 5% dextrose injection are reported. Methods. Triplicate solutions of isoniazid (0.5 and 6.0 mg/mL) in the 2 diluents were prepared in ethylene and propylene copolymer i.v. containers and stored under light protection at room temperature (20–25 °C) or under refrigeration (2–8 °C). Standard aliquots were removed from each solution at time points up to 72 hours and analyzed via high-performance liquid chromatography (HPLC). Stability was defined as retention of >90% of the initial isoniazid concentration; pH, osmolality, and visual appearance were assessed. Results. Isoniazid 0.5- and 6.0-mg/mL solutions in 0.9% sodium chloride injection were stable for up to 72 hours at room temperature or under refrigeration. HPLC analysis of isoniazid 0.5-mg/mL solutions in 5% dextrose injection revealed a decrease to less than 90% of the initial concentration at 8 hours at room temperature and at 30 hours under refrigeration. Isoniazid 6.0-mg/mL solutions in 5% dextrose injection were stable for 24 hours at room temperature and for 48 hours under refrigeration. The pH, osmolality, and visual appearance of the solutions were not affected. Conclusion. Isoniazid solutions of 0.5 and 6.0 mg/mL in 0.9% sodium chloride injection were stable under light protection for up to 72 hours when stored at room temperature or under refrigeration. Isoniazid injection was less stable in 5% dextrose injection, especially at a concentration of 0.5 mg/mL at room temperature.

KW - Intravenous

KW - Isoniazid

KW - Stability

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DO - 10.2146/ajhp170268

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VL - 75

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JF - American Journal of Health-System Pharmacy

SN - 1079-2082

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Lee JH, Moriyama B, Henning SA, Danner RL, Walsh TJ, Penzak SR et al. Stability of isoniazid injection in i.v. solutions. American Journal of Health-System Pharmacy. 2018 May 15;75(10):622-626. https://doi.org/10.2146/ajhp170268