Specific lectin biomarkers for isolation of human pluripotent stem cells identified through array-based glycomic analysis

Yu Chieh Wang, Masato Nakagawa, Ibon Garitaonandia, Ileana Slavin, Gulsah Altun, Robert M. Lacharite, Kristopher L. Nazor, Ha T. Tran, Candace L. Lynch, Trevor R. Leonardo, Ying Liu, Suzanne E. Peterson, Louise C. Laurent, Shinya Yamanaka, Jeanne F. Loring

Research output: Contribution to journalArticle

59 Scopus citations

Abstract

Rapid and dependable methods for isolating human pluripotent stem cell (hPSC) populations are urgently needed for quality control in basic research and in cell-based therapy applications. Using lectin arrays, we analyzed glycoproteins extracted from 26 hPSC samples and 22 differentiated cell samples, and identified a small group of lectins with distinctive binding signatures that were sufficient to distinguish hPSCs from a variety of non-pluripotent cell types. These specific biomarkers were shared by all the 12 human embryonic stem cell and the 14 human induced pluripotent stem cell samples examined, regardless of the laboratory of origin, the culture conditions, the somatic cell type reprogrammed, or the reprogramming method used. We demonstrated a practical application of specific lectin binding by detecting hPSCs within a differentiated cell population with lectin-mediated staining followed by fluorescence microscopy and flow cytometry, and by enriching and purging viable hPSCs from mixed cell populations using lectin-mediated cell separation. Global gene expression analysis showed pluripotency-associated differential expression of specific fucosyltransferases and sialyltransferases, which may underlie these differences in protein glycosylation and lectin binding. Taken together, our results show that protein glycosylation differs considerably between pluripotent and non-pluripotent cells, and demonstrate that lectins may be used as biomarkers to monitor pluripotency in stem cell populations and for removal of viable hPSCs from mixed cell populations.

Original languageEnglish
Pages (from-to)1551-1563
Number of pages13
JournalCell Research
Volume21
Issue number11
DOIs
StatePublished - Nov 2011

Keywords

  • embryonic stem cells
  • glycosylation
  • human pluripotent stem cell
  • induced pluripotent stem cells
  • lectins
  • pluripotency biomarkers

Fingerprint Dive into the research topics of 'Specific lectin biomarkers for isolation of human pluripotent stem cells identified through array-based glycomic analysis'. Together they form a unique fingerprint.

  • Cite this

    Wang, Y. C., Nakagawa, M., Garitaonandia, I., Slavin, I., Altun, G., Lacharite, R. M., Nazor, K. L., Tran, H. T., Lynch, C. L., Leonardo, T. R., Liu, Y., Peterson, S. E., Laurent, L. C., Yamanaka, S., & Loring, J. F. (2011). Specific lectin biomarkers for isolation of human pluripotent stem cells identified through array-based glycomic analysis. Cell Research, 21(11), 1551-1563. https://doi.org/10.1038/cr.2011.148