Abstract
We describe spatially localized two-photon excitation in scattering media. Using femtosecond pulses at 770 nm from a Ti : Sapphire laser, we were able to excite fluorophores in capillary tubes under up to 1.5 mm of 0.5% intralipid. Displacement of the laser beam relative to the embedded samples indicates that highly localized excitation was possible with two-photon excitation, whereas one-photon excitation resulted in loss of spatial resolution due to excitation by the diffusely scattered photons. These results indicate that two-photon excitation in the scattering solution is due only to the ballistic photons, a result confirmed by frequency-domain time-resolved measurements. Selective excitation of adjacent embedded samples was found possible for two but not one-photon excitation.
Original language | English |
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Pages (from-to) | 303-310 |
Number of pages | 8 |
Journal | Biospectroscopy |
Volume | 4 |
Issue number | 5 |
DOIs | |
State | Published - 1998 |
Keywords
- Ballistic photons
- Fluorescence
- Optical tomography
- Photon migration
- Time-resolved fluorescence
- Two-photon excitation