Spatially localized ballistic two-photon excitation in scattering media

Henryk Szmacinski, Ignacy Gryczynski, Joseph R. Lakowicz

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


We describe spatially localized two-photon excitation in scattering media. Using femtosecond pulses at 770 nm from a Ti : Sapphire laser, we were able to excite fluorophores in capillary tubes under up to 1.5 mm of 0.5% intralipid. Displacement of the laser beam relative to the embedded samples indicates that highly localized excitation was possible with two-photon excitation, whereas one-photon excitation resulted in loss of spatial resolution due to excitation by the diffusely scattered photons. These results indicate that two-photon excitation in the scattering solution is due only to the ballistic photons, a result confirmed by frequency-domain time-resolved measurements. Selective excitation of adjacent embedded samples was found possible for two but not one-photon excitation.

Original languageEnglish
Pages (from-to)303-310
Number of pages8
Issue number5
StatePublished - 1998


  • Ballistic photons
  • Fluorescence
  • Optical tomography
  • Photon migration
  • Time-resolved fluorescence
  • Two-photon excitation


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