Solvatochromic dye LDS 798 as microviscosity and pH probe

Hung Doan; Marlius Castillo; Micheline Bejjani; Zhangatay Nurekeyev; Sergei V. Dzyuba; Ignacy Gryczynski; Zygmunt Gryczynski; Sangram Raut

doi:10.1039/c7cp05874h

Solvatochromic dye LDS 798 as microviscosity and pH probe

Hung Doan, Marlius Castillo, Micheline Bejjani, Zhangatay Nurekeyev, Sergei V. Dzyuba, Ignacy Gryczynski, Zygmunt Gryczynski, Sangram Raut

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18 Scopus citations

Abstract

Styryl dyes, specifically LDS group dyes, are known solvatochromic and electrochromic probes for monitoring mitochondrial potential in cellular environments. However, the ability of these dyes to respond to fluctuations in viscosity, pH and temperature has not been established. In this study, we demonstrated that LDS 798 (also known as Styryl-11) can sense environmental viscosity (via fluorescence lifetime changes) as well as pH changes (ratiometric intensity change) in the absence of polarity variations. Polarity changes can be probed by spectral changes using LDS 798. Therefore, all properties of the media should be considered, when these types of dyes are used as electrochromic/solvatochromic sensors in cellular environments.

Original language	English
Pages (from-to)	29934-29939
Number of pages	6
Journal	Physical Chemistry Chemical Physics
Volume	19
Issue number	44
DOIs	https://doi.org/10.1039/c7cp05874h
State	Published - 2017

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title = "Solvatochromic dye LDS 798 as microviscosity and pH probe",

abstract = "Styryl dyes, specifically LDS group dyes, are known solvatochromic and electrochromic probes for monitoring mitochondrial potential in cellular environments. However, the ability of these dyes to respond to fluctuations in viscosity, pH and temperature has not been established. In this study, we demonstrated that LDS 798 (also known as Styryl-11) can sense environmental viscosity (via fluorescence lifetime changes) as well as pH changes (ratiometric intensity change) in the absence of polarity variations. Polarity changes can be probed by spectral changes using LDS 798. Therefore, all properties of the media should be considered, when these types of dyes are used as electrochromic/solvatochromic sensors in cellular environments.",

author = "Hung Doan and Marlius Castillo and Micheline Bejjani and Zhangatay Nurekeyev and Dzyuba, {Sergei V.} and Ignacy Gryczynski and Zygmunt Gryczynski and Sangram Raut",

note = "Funding Information: This work was supported in part by NSF (CBET 1403226 to S. V. D. and CBET 1264608 to I. G.) and NIH (R21EB017985 and R01EB12003 to Z. G.). Publisher Copyright: {\textcopyright} 2017 the Owner Societies.",

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doi = "10.1039/c7cp05874h",

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T1 - Solvatochromic dye LDS 798 as microviscosity and pH probe

AU - Doan, Hung

AU - Castillo, Marlius

AU - Bejjani, Micheline

AU - Nurekeyev, Zhangatay

AU - Dzyuba, Sergei V.

AU - Gryczynski, Ignacy

AU - Gryczynski, Zygmunt

AU - Raut, Sangram

N1 - Funding Information: This work was supported in part by NSF (CBET 1403226 to S. V. D. and CBET 1264608 to I. G.) and NIH (R21EB017985 and R01EB12003 to Z. G.). Publisher Copyright: © 2017 the Owner Societies.

PY - 2017

Y1 - 2017

N2 - Styryl dyes, specifically LDS group dyes, are known solvatochromic and electrochromic probes for monitoring mitochondrial potential in cellular environments. However, the ability of these dyes to respond to fluctuations in viscosity, pH and temperature has not been established. In this study, we demonstrated that LDS 798 (also known as Styryl-11) can sense environmental viscosity (via fluorescence lifetime changes) as well as pH changes (ratiometric intensity change) in the absence of polarity variations. Polarity changes can be probed by spectral changes using LDS 798. Therefore, all properties of the media should be considered, when these types of dyes are used as electrochromic/solvatochromic sensors in cellular environments.

AB - Styryl dyes, specifically LDS group dyes, are known solvatochromic and electrochromic probes for monitoring mitochondrial potential in cellular environments. However, the ability of these dyes to respond to fluctuations in viscosity, pH and temperature has not been established. In this study, we demonstrated that LDS 798 (also known as Styryl-11) can sense environmental viscosity (via fluorescence lifetime changes) as well as pH changes (ratiometric intensity change) in the absence of polarity variations. Polarity changes can be probed by spectral changes using LDS 798. Therefore, all properties of the media should be considered, when these types of dyes are used as electrochromic/solvatochromic sensors in cellular environments.

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DO - 10.1039/c7cp05874h

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JO - Physical Chemistry Chemical Physics

JF - Physical Chemistry Chemical Physics

SN - 1463-9076

IS - 44

ER -

Solvatochromic dye LDS 798 as microviscosity and pH probe

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