TY - JOUR
T1 - Solvatochromic dye LDS 798 as microviscosity and pH probe
AU - Doan, Hung
AU - Castillo, Marlius
AU - Bejjani, Micheline
AU - Nurekeyev, Zhangatay
AU - Dzyuba, Sergei V.
AU - Gryczynski, Ignacy
AU - Gryczynski, Zygmunt
AU - Raut, Sangram
N1 - Funding Information:
This work was supported in part by NSF (CBET 1403226 to S. V. D. and CBET 1264608 to I. G.) and NIH (R21EB017985 and R01EB12003 to Z. G.).
Publisher Copyright:
© 2017 the Owner Societies.
PY - 2017
Y1 - 2017
N2 - Styryl dyes, specifically LDS group dyes, are known solvatochromic and electrochromic probes for monitoring mitochondrial potential in cellular environments. However, the ability of these dyes to respond to fluctuations in viscosity, pH and temperature has not been established. In this study, we demonstrated that LDS 798 (also known as Styryl-11) can sense environmental viscosity (via fluorescence lifetime changes) as well as pH changes (ratiometric intensity change) in the absence of polarity variations. Polarity changes can be probed by spectral changes using LDS 798. Therefore, all properties of the media should be considered, when these types of dyes are used as electrochromic/solvatochromic sensors in cellular environments.
AB - Styryl dyes, specifically LDS group dyes, are known solvatochromic and electrochromic probes for monitoring mitochondrial potential in cellular environments. However, the ability of these dyes to respond to fluctuations in viscosity, pH and temperature has not been established. In this study, we demonstrated that LDS 798 (also known as Styryl-11) can sense environmental viscosity (via fluorescence lifetime changes) as well as pH changes (ratiometric intensity change) in the absence of polarity variations. Polarity changes can be probed by spectral changes using LDS 798. Therefore, all properties of the media should be considered, when these types of dyes are used as electrochromic/solvatochromic sensors in cellular environments.
UR - http://www.scopus.com/inward/record.url?scp=85034596446&partnerID=8YFLogxK
U2 - 10.1039/c7cp05874h
DO - 10.1039/c7cp05874h
M3 - Article
C2 - 29090298
AN - SCOPUS:85034596446
VL - 19
SP - 29934
EP - 29939
JO - Physical Chemistry Chemical Physics
JF - Physical Chemistry Chemical Physics
SN - 1463-9076
IS - 44
ER -