SMRT Sequencing Enables High-Throughput Identification of Novel AAVs from Capsid Shuffling and Directed Evolution

Keyphrases

• AAV Capsid 66%
• Bulk Tissue 33%
• Capsid 100%
• Capsid Engineering 66%
• Capsid Gene 33%
• Capsid Library 66%
• Capsid Modification 33%
• Central Nervous System 33%
• Directed Evolution 100%
• Enrichment Step 33%
• High-throughput 33%
• High-throughput Identification 100%
• Isolated nuclei 33%
• Library Construction 33%
• Library Strategy 33%
• Methodological Improvements 33%
• Next-generation Sequencing 33%
• Nonhuman Primate 33%
• Peptide Insertion 33%
• Selection Strategy 33%
• Short Reads 33%
• Single-molecule Sequencing 100%
• Translational Application 33%
• Variant Identification 33%

Immunology and Microbiology

• Adeno-Associated Virus 100%
• Capsid 100%
• Central Nervous System 10%
• Mouse 10%
• Next Generation Sequencing 10%
• Volume 10%

Biochemistry, Genetics and Molecular Biology

• Capsid 100%
• Mouse 10%
• Next Generation Sequencing 10%
• Single Molecule Real Time Sequencing 100%
• Volume 10%