@inproceedings{250175ffd54e4bc99bb9c413f1d9a358,
title = "Site-to-site diffusion in proteins as observed by energy transfer and frequency-domain fluorometry",
abstract = "We report measurements of site-to-site diffusion in proteins, using frequency-domain measurements of time-dependent energy transfer. The possibility of such measurements is shown from simulations which demonstrate that donor-to-acceptor (D-to-A) diffusion alters the donor frequency response, and that this effect is observable in the presence of a distribution of distances. For decay times typical of tryptophan fluorescence, the simulations indicate D-to-A diffusion coefficients can be measured ranging from 10-7 to 10-5 cm2/s. This possibility was verified by studies of a methylene-chain linked D-A pairs in solutions of varying viscosity. D-to-A diffusion was also measured for acceptor-labeled melittin in the random coil and α-helical states. Unfolding of troponin I results in increased D-A diffusion. Surprisingly, more rapid diffusion was observed for melittin in the α-helical state, but over a limited range of distances.",
author = "Lakowicz, {Joseph R.} and Ignacy Gryczynski and Jozef Kusba and Wiczk, {Wieslaw M.} and Henryk Szmacinski and Johnson, {Michael L.}",
year = "1992",
language = "English",
isbn = "0819407860",
series = "Proceedings of SPIE - The International Society for Optical Engineering",
publisher = "Publ by Int Soc for Optical Engineering",
pages = "196--211",
booktitle = "Proceedings of SPIE - The International Society for Optical Engineering",
note = "Time-Resolved Laser Spectroscopy in Biochemistry III ; Conference date: 20-01-1992 Through 22-01-1992",
}