TY - JOUR
T1 - Single Nucleotide Polymorphisms in Chemosensory Pathway Genes GNB3, TAS2R19, and TAS2R38 Are Associated with Chronic Rhinosinusitis
AU - Purnell, Phillip R.
AU - Addicks, Benjamin L.
AU - Zalzal, Habib G.
AU - Shapiro, Scott
AU - Wen, Sijin
AU - Ramadan, Hassan H.
AU - Setola, Vincent
AU - Siderovski, David P.
N1 - Funding Information:
This research was supported in part by the National Institute of General Medical Sciences of the National Institutes of Health under Award Number 5U54GM104942-03 to the West Virginia Clinical and Translational Science Institute (to B.L.A. and D.P.S.). Funding support was also provided by the WVU E.J. Van Liere Endowed Medicine Professorship (to D.P.S.). Neither of these sources participated in the preparation of data or the paper.
Publisher Copyright:
© 2019 S. Karger AG, Basel. Copyright: All rights reserved.
PY - 2019/8/1
Y1 - 2019/8/1
N2 - Background: Chronic rhinosinusitis (CRS) is a multifaceted disease with a significant genetic component. The importance of taste receptor signaling has recently been highlighted in CRS; single nucleotide polymorphisms (SNPs) of bitter tastant-responsive G-protein-coupled receptors have been linked with CRS and with altered innate immune responses to multiple bacterially derived signals. Objective: To determine in CRS the frequency of six SNPs in genes with known bitter tastant signaling function. Methods: Genomic DNA was isolated from 74 CRS volunteers in West Virginia, and allele frequency was determined and compared with demographically matched data from the 1,000 Genomes database. Results: For two SNPs in a gene recently associated with bitterant signaling regulation, RGS21, there were no associations with CRS (although the frequency of the minor allele of RGS21, rs7528947, was seen to increase with increasing Lund-Mackay CT staging score). Two TAS2R bitter taste receptor gene variants (TAS2R19 rs10772420 and TAS2R38 rs713598), identified in prior CRS genetics studies, were found to have similar associations in this study. Conclusion: Unique to our study is the establishment of an association between CRS in this patient population and GNB3 SNP rs5443, a variation in an established G protein component downstream of bitterant receptor signal transduction.
AB - Background: Chronic rhinosinusitis (CRS) is a multifaceted disease with a significant genetic component. The importance of taste receptor signaling has recently been highlighted in CRS; single nucleotide polymorphisms (SNPs) of bitter tastant-responsive G-protein-coupled receptors have been linked with CRS and with altered innate immune responses to multiple bacterially derived signals. Objective: To determine in CRS the frequency of six SNPs in genes with known bitter tastant signaling function. Methods: Genomic DNA was isolated from 74 CRS volunteers in West Virginia, and allele frequency was determined and compared with demographically matched data from the 1,000 Genomes database. Results: For two SNPs in a gene recently associated with bitterant signaling regulation, RGS21, there were no associations with CRS (although the frequency of the minor allele of RGS21, rs7528947, was seen to increase with increasing Lund-Mackay CT staging score). Two TAS2R bitter taste receptor gene variants (TAS2R19 rs10772420 and TAS2R38 rs713598), identified in prior CRS genetics studies, were found to have similar associations in this study. Conclusion: Unique to our study is the establishment of an association between CRS in this patient population and GNB3 SNP rs5443, a variation in an established G protein component downstream of bitterant receptor signal transduction.
KW - Bitterant signaling
KW - Chronic rhinosinusitis
KW - Genetics
KW - Innate immune response
KW - Single nucleotide polymorphism
UR - http://www.scopus.com/inward/record.url?scp=85066959776&partnerID=8YFLogxK
U2 - 10.1159/000499875
DO - 10.1159/000499875
M3 - Article
C2 - 31137020
AN - SCOPUS:85066959776
SN - 1018-2438
VL - 180
SP - 72
EP - 77
JO - International Archives of Allergy and Immunology
JF - International Archives of Allergy and Immunology
IS - 1
ER -