Single molecule immunoassay on plasmonic platforms

R. Luchowski, E. G. Matveeva, T. Shtoyko, P. Sarkar, L. D. Patsenker, O. P. Klochko, E. A. Terpetschnig, Julian Borejdo, I. Akopova, Zygmunt Gryczynski, Ignacy Gryczynski

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

We examined the photophysical properties of the new near infrared (NIR) fluorescent label SeTau-665 on a plasmonic platform of self- assembled colloidal structures (SACS) of silver prepared on a semitransparent silver film. A SeTau-665 immunoassay was performed on this platform and a control glass slide. The fluorescence properties of this label substantially change due to plasmonic interactions. While the average brightness increase of SeTau 665 in ensemble measurements was about 70-fold, fluorescence enhancements up to four-hundred times were observed on certain "hot spots" for single molecule measurements. The intensity increase is strongly correlated with a simultaneous decrease in fluorescence lifetime in these "hot spots". The large increase in brightness allows the reduction of the excitation power resulting in a reduced background and increased photostability. The remarkable fluorescence enhancements observed for SeTau 665 on our plasmonic platform should allow to substantially improve single molecule detection and to reduce the detection limits in sensing devices.

Original languageEnglish
Pages (from-to)96-102
Number of pages7
JournalCurrent Pharmaceutical Biotechnology
Volume11
Issue number1
DOIs
StatePublished - 1 Jan 2010

Keywords

  • Fluorescence enhancement
  • Fluorescence lifetime imaging microscopy
  • Plasmonic platforms
  • Single molecule detection

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    Luchowski, R., Matveeva, E. G., Shtoyko, T., Sarkar, P., Patsenker, L. D., Klochko, O. P., Terpetschnig, E. A., Borejdo, J., Akopova, I., Gryczynski, Z., & Gryczynski, I. (2010). Single molecule immunoassay on plasmonic platforms. Current Pharmaceutical Biotechnology, 11(1), 96-102. https://doi.org/10.2174/138920110790725384