Single molecule immunoassay on plasmonic platforms

R. Luchowski; E. G. Matveeva; T. Shtoyko; P. Sarkar; L. D. Patsenker; O. P. Klochko; E. A. Terpetschnig; J. Borejdo; I. Akopova; Z. Gryczynski; I. Gryczynski

doi:10.2174/138920110790725384

Single molecule immunoassay on plasmonic platforms

R. Luchowski, E. G. Matveeva, T. Shtoyko, P. Sarkar, L. D. Patsenker, O. P. Klochko, E. A. Terpetschnig, J. Borejdo, I. Akopova, Z. Gryczynski, I. Gryczynski

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

We examined the photophysical properties of the new near infrared (NIR) fluorescent label SeTau-665 on a plasmonic platform of self- assembled colloidal structures (SACS) of silver prepared on a semitransparent silver film. A SeTau-665 immunoassay was performed on this platform and a control glass slide. The fluorescence properties of this label substantially change due to plasmonic interactions. While the average brightness increase of SeTau 665 in ensemble measurements was about 70-fold, fluorescence enhancements up to four-hundred times were observed on certain "hot spots" for single molecule measurements. The intensity increase is strongly correlated with a simultaneous decrease in fluorescence lifetime in these "hot spots". The large increase in brightness allows the reduction of the excitation power resulting in a reduced background and increased photostability. The remarkable fluorescence enhancements observed for SeTau 665 on our plasmonic platform should allow to substantially improve single molecule detection and to reduce the detection limits in sensing devices.

Original language	English
Pages (from-to)	96-102
Number of pages	7
Journal	Current Pharmaceutical Biotechnology
Volume	11
Issue number	1
DOIs	https://doi.org/10.2174/138920110790725384
State	Published - Jan 2010

Keywords

Fluorescence enhancement
Fluorescence lifetime imaging microscopy
Plasmonic platforms
Single molecule detection

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title = "Single molecule immunoassay on plasmonic platforms",

abstract = "We examined the photophysical properties of the new near infrared (NIR) fluorescent label SeTau-665 on a plasmonic platform of self- assembled colloidal structures (SACS) of silver prepared on a semitransparent silver film. A SeTau-665 immunoassay was performed on this platform and a control glass slide. The fluorescence properties of this label substantially change due to plasmonic interactions. While the average brightness increase of SeTau 665 in ensemble measurements was about 70-fold, fluorescence enhancements up to four-hundred times were observed on certain {"}hot spots{"} for single molecule measurements. The intensity increase is strongly correlated with a simultaneous decrease in fluorescence lifetime in these {"}hot spots{"}. The large increase in brightness allows the reduction of the excitation power resulting in a reduced background and increased photostability. The remarkable fluorescence enhancements observed for SeTau 665 on our plasmonic platform should allow to substantially improve single molecule detection and to reduce the detection limits in sensing devices.",

keywords = "Fluorescence enhancement, Fluorescence lifetime imaging microscopy, Plasmonic platforms, Single molecule detection",

author = "R. Luchowski and Matveeva, {E. G.} and T. Shtoyko and P. Sarkar and Patsenker, {L. D.} and Klochko, {O. P.} and Terpetschnig, {E. A.} and J. Borejdo and I. Akopova and Z. Gryczynski and I. Gryczynski",

year = "2010",

month = jan,

doi = "10.2174/138920110790725384",

language = "English",

volume = "11",

pages = "96--102",

journal = "Current Pharmaceutical Biotechnology",

issn = "1389-2010",

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AU - Luchowski, R.

AU - Matveeva, E. G.

AU - Shtoyko, T.

AU - Sarkar, P.

AU - Patsenker, L. D.

AU - Klochko, O. P.

AU - Terpetschnig, E. A.

AU - Borejdo, J.

AU - Akopova, I.

AU - Gryczynski, Z.

AU - Gryczynski, I.

PY - 2010/1

Y1 - 2010/1

N2 - We examined the photophysical properties of the new near infrared (NIR) fluorescent label SeTau-665 on a plasmonic platform of self- assembled colloidal structures (SACS) of silver prepared on a semitransparent silver film. A SeTau-665 immunoassay was performed on this platform and a control glass slide. The fluorescence properties of this label substantially change due to plasmonic interactions. While the average brightness increase of SeTau 665 in ensemble measurements was about 70-fold, fluorescence enhancements up to four-hundred times were observed on certain "hot spots" for single molecule measurements. The intensity increase is strongly correlated with a simultaneous decrease in fluorescence lifetime in these "hot spots". The large increase in brightness allows the reduction of the excitation power resulting in a reduced background and increased photostability. The remarkable fluorescence enhancements observed for SeTau 665 on our plasmonic platform should allow to substantially improve single molecule detection and to reduce the detection limits in sensing devices.

AB - We examined the photophysical properties of the new near infrared (NIR) fluorescent label SeTau-665 on a plasmonic platform of self- assembled colloidal structures (SACS) of silver prepared on a semitransparent silver film. A SeTau-665 immunoassay was performed on this platform and a control glass slide. The fluorescence properties of this label substantially change due to plasmonic interactions. While the average brightness increase of SeTau 665 in ensemble measurements was about 70-fold, fluorescence enhancements up to four-hundred times were observed on certain "hot spots" for single molecule measurements. The intensity increase is strongly correlated with a simultaneous decrease in fluorescence lifetime in these "hot spots". The large increase in brightness allows the reduction of the excitation power resulting in a reduced background and increased photostability. The remarkable fluorescence enhancements observed for SeTau 665 on our plasmonic platform should allow to substantially improve single molecule detection and to reduce the detection limits in sensing devices.

KW - Fluorescence enhancement

KW - Fluorescence lifetime imaging microscopy

KW - Plasmonic platforms

KW - Single molecule detection

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DO - 10.2174/138920110790725384

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SN - 1389-2010

VL - 11

SP - 96

EP - 102

JO - Current Pharmaceutical Biotechnology

JF - Current Pharmaceutical Biotechnology

IS - 1

ER -

Single molecule immunoassay on plasmonic platforms

Abstract

Keywords

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