Single copy gene for the chicken non-histone chromosomal protein HMG-17

D. Landsman, Srikantha Thyagarajan, M. Bustin

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A chicken genomic library was screened with the human cDNA encoding the non-histone chromosomal protein HMG-17 and a 4565-base pair fragment containing the entire gene encoding this protein was isolated and characterized. Sequence analysis of the fragment revealed that from the start to end of transcription, the HMG-17 gene is 3293 base pairs long and is comprised of 6 exons ranging in size from 30 to 890 base pairs. Upstream of the putative cap site are both a CAAT box and a TATA box as well as several Sp1 binding sites. The gene has an extremely high content of G and C residues (75%) in a 1150-base pair fragment starting 500 base pairs from the putative cap site. This region satisfies the definition of an HpaII tiny fragment island. Southern analysis indicated that there is a single copy of this gene in chickens, whereas Northern analysis revealed that a single transcript is being synthesized from this gene. A comparison of the chicken and human cDNA and protein sequences and subsequent calculation of the evolutionary rates indicated that HMG-17 is a slowly evolving gene. The present article, which is the first study on the isolation and characterization of a complete gene coding for a high mobility group non-histone protein, indicates that the gene has features characteristic of housekeeping genes.

Original languageEnglish
Pages (from-to)3917-3923
Number of pages7
JournalJournal of Biological Chemistry
Volume263
Issue number8
StatePublished - 1 Jan 1988

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HMGN2 Protein
Non Histone Chromosomal Proteins
Chickens
Base Pairing
Genes
Complementary DNA
TATA Box
Proteins
Genomic Library
Essential Genes
Base Composition
Islands
Gene encoding
Sequence Analysis
Exons
Transcription
Binding Sites

Cite this

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abstract = "A chicken genomic library was screened with the human cDNA encoding the non-histone chromosomal protein HMG-17 and a 4565-base pair fragment containing the entire gene encoding this protein was isolated and characterized. Sequence analysis of the fragment revealed that from the start to end of transcription, the HMG-17 gene is 3293 base pairs long and is comprised of 6 exons ranging in size from 30 to 890 base pairs. Upstream of the putative cap site are both a CAAT box and a TATA box as well as several Sp1 binding sites. The gene has an extremely high content of G and C residues (75{\%}) in a 1150-base pair fragment starting 500 base pairs from the putative cap site. This region satisfies the definition of an HpaII tiny fragment island. Southern analysis indicated that there is a single copy of this gene in chickens, whereas Northern analysis revealed that a single transcript is being synthesized from this gene. A comparison of the chicken and human cDNA and protein sequences and subsequent calculation of the evolutionary rates indicated that HMG-17 is a slowly evolving gene. The present article, which is the first study on the isolation and characterization of a complete gene coding for a high mobility group non-histone protein, indicates that the gene has features characteristic of housekeeping genes.",
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Single copy gene for the chicken non-histone chromosomal protein HMG-17. / Landsman, D.; Thyagarajan, Srikantha; Bustin, M.

In: Journal of Biological Chemistry, Vol. 263, No. 8, 01.01.1988, p. 3917-3923.

Research output: Contribution to journalArticle

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