Simultaneous quantification of mitochondrial DNA copy number and deletion ratio: A multiplex real-time PCR assay

Nicole R. Phillips, Marc L. Sprouse, Rhonda K. Roby

Research output: Contribution to journalArticle

69 Scopus citations

Abstract

Mitochondrial dysfunction is implicated in a vast array of diseases and conditions, such as Alzheimer's disease, cancer, and aging. Alterations in mitochondrial DNA (mtDNA) may provide insight into the processes that either initiate or propagate this dysfunction. Here, we describe a unique multiplex assay which simultaneously provides assessments of mtDNA copy number and the proportion of genomes with common large deletions by targeting two mitochondrial sites and one nuclear locus. This probe-based, single-tube multiplex provides high specificity while eliminating well-to-well variability that results from assaying nuclear and mitochondrial targets individually.

Original languageEnglish
Article number3887
JournalScientific Reports
Volume4
DOIs
StatePublished - 27 Jan 2014

Fingerprint Dive into the research topics of 'Simultaneous quantification of mitochondrial DNA copy number and deletion ratio: A multiplex real-time PCR assay'. Together they form a unique fingerprint.

  • Cite this