@article{bbd6e794c885406eb25be8322076dc58,
title = "Simultaneous quantification of mitochondrial DNA copy number and deletion ratio: A multiplex real-time PCR assay",
abstract = "Mitochondrial dysfunction is implicated in a vast array of diseases and conditions, such as Alzheimer's disease, cancer, and aging. Alterations in mitochondrial DNA (mtDNA) may provide insight into the processes that either initiate or propagate this dysfunction. Here, we describe a unique multiplex assay which simultaneously provides assessments of mtDNA copy number and the proportion of genomes with common large deletions by targeting two mitochondrial sites and one nuclear locus. This probe-based, single-tube multiplex provides high specificity while eliminating well-to-well variability that results from assaying nuclear and mitochondrial targets individually.",
author = "Phillips, {Nicole R.} and Sprouse, {Marc L.} and Roby, {Rhonda K.}",
note = "Funding Information: We would like to acknowledge the contribution of Dr. Carlos Moraes and Dr. Milena Pinto at the University of Miami Miller School of Medicine for their donation of the cybrid DNA. Additionally, we would like to acknowledge Dr. Michael Allen and his laboratory at the University of North Texas Health Science Center for their assistance with the ddPCR experiments. This project was funded in part by the following awards: Alzheimer{\textquoteright}s Association NIRG-12-242728; UNTHSC Intramural Seed Grant, 2012–2013; National Institute of Aging, Training in the Neurobiology of Aging T32 AG 020494.",
year = "2014",
month = jan,
day = "27",
doi = "10.1038/srep03887",
language = "English",
volume = "4",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
}