Simple protocols for typing forensic biological evidence: Chemiluminescent detection for human DNA quantitation and restriction fragment length polymorphism (RELP) analyses and manual typing of polymerase chain reaction (PCR) amplified polymorphisms

Bruce Budowle, F. Samuel Baechtel, Catherine T. Comey, Alan M. Giusti, Leonard Klevan

Research output: Contribution to journalArticle

58 Scopus citations

Abstract

Methods for identity testing are described that enable extraction of DNA from biological samples, determination of the quantity of human DNA, and genetic analyses of the materials using restriction fragment length polymorphism (RFLP) typing and/or amplified fragment length polymorphism (AMP‐FLP) typing of PCR products. The salient features of the procedures are simplicity, manual typing, nonradioactive chemiluminescent assays or silver staining for detection, and low cost. Most application‐oriented laboratories involved in forensic and/or paternity testing should be able to implement these procedures.

Original languageEnglish
Pages (from-to)1559-1567
Number of pages9
JournalELECTROPHORESIS
Volume16
Issue number1
DOIs
StatePublished - 1 Jan 1995

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Keywords

  • Chemiluminescence
  • DNA extraction
  • DNA quantitation
  • Multiplex amplification
  • Polymerase chain reaction
  • Restriction fragment length polymorphism
  • Slot blot

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