Simple protocols for typing forensic biological evidence: Chemiluminescent detection for human DNA quantitation and restriction fragment length polymorphism (RELP) analyses and manual typing of polymerase chain reaction (PCR) amplified polymorphisms

Bruce Budowle; F. Samuel Baechtel; Catherine T. Comey; Alan M. Giusti; Leonard Klevan

doi:10.1002/elps.11501601259

Simple protocols for typing forensic biological evidence: Chemiluminescent detection for human DNA quantitation and restriction fragment length polymorphism (RELP) analyses and manual typing of polymerase chain reaction (PCR) amplified polymorphisms

Bruce Budowle, F. Samuel Baechtel, Catherine T. Comey, Alan M. Giusti, Leonard Klevan

Research output: Contribution to journal › Article › peer-review

60 Scopus citations

Abstract

Methods for identity testing are described that enable extraction of DNA from biological samples, determination of the quantity of human DNA, and genetic analyses of the materials using restriction fragment length polymorphism (RFLP) typing and/or amplified fragment length polymorphism (AMP‐FLP) typing of PCR products. The salient features of the procedures are simplicity, manual typing, nonradioactive chemiluminescent assays or silver staining for detection, and low cost. Most application‐oriented laboratories involved in forensic and/or paternity testing should be able to implement these procedures.

Original language	English
Pages (from-to)	1559-1567
Number of pages	9
Journal	ELECTROPHORESIS
Volume	16
Issue number	1
DOIs	https://doi.org/10.1002/elps.11501601259
State	Published - 1 Jan 1995

Keywords

Chemiluminescence
DNA extraction
DNA quantitation
Multiplex amplification
Polymerase chain reaction
Restriction fragment length polymorphism
Slot blot

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Budowle, B., Baechtel, F. S., Comey, C. T., Giusti, A. M., & Klevan, L. (1995). Simple protocols for typing forensic biological evidence: Chemiluminescent detection for human DNA quantitation and restriction fragment length polymorphism (RELP) analyses and manual typing of polymerase chain reaction (PCR) amplified polymorphisms. ELECTROPHORESIS, 16(1), 1559-1567. https://doi.org/10.1002/elps.11501601259

Budowle, Bruce ; Baechtel, F. Samuel ; Comey, Catherine T. et al. / Simple protocols for typing forensic biological evidence : Chemiluminescent detection for human DNA quantitation and restriction fragment length polymorphism (RELP) analyses and manual typing of polymerase chain reaction (PCR) amplified polymorphisms. In: ELECTROPHORESIS. 1995 ; Vol. 16, No. 1. pp. 1559-1567.

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abstract = "Methods for identity testing are described that enable extraction of DNA from biological samples, determination of the quantity of human DNA, and genetic analyses of the materials using restriction fragment length polymorphism (RFLP) typing and/or amplified fragment length polymorphism (AMP‐FLP) typing of PCR products. The salient features of the procedures are simplicity, manual typing, nonradioactive chemiluminescent assays or silver staining for detection, and low cost. Most application‐oriented laboratories involved in forensic and/or paternity testing should be able to implement these procedures.",

keywords = "Chemiluminescence, DNA extraction, DNA quantitation, Multiplex amplification, Polymerase chain reaction, Restriction fragment length polymorphism, Slot blot",

author = "Bruce Budowle and Baechtel, {F. Samuel} and Comey, {Catherine T.} and Giusti, {Alan M.} and Leonard Klevan",

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Budowle, B, Baechtel, FS, Comey, CT, Giusti, AM & Klevan, L 1995, 'Simple protocols for typing forensic biological evidence: Chemiluminescent detection for human DNA quantitation and restriction fragment length polymorphism (RELP) analyses and manual typing of polymerase chain reaction (PCR) amplified polymorphisms', ELECTROPHORESIS, vol. 16, no. 1, pp. 1559-1567. https://doi.org/10.1002/elps.11501601259

Simple protocols for typing forensic biological evidence : Chemiluminescent detection for human DNA quantitation and restriction fragment length polymorphism (RELP) analyses and manual typing of polymerase chain reaction (PCR) amplified polymorphisms. / Budowle, Bruce; Baechtel, F. Samuel; Comey, Catherine T. et al.

In: ELECTROPHORESIS, Vol. 16, No. 1, 01.01.1995, p. 1559-1567.

Research output: Contribution to journal › Article › peer-review

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KW - DNA quantitation

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KW - Polymerase chain reaction

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Budowle B, Baechtel FS, Comey CT, Giusti AM, Klevan L. Simple protocols for typing forensic biological evidence: Chemiluminescent detection for human DNA quantitation and restriction fragment length polymorphism (RELP) analyses and manual typing of polymerase chain reaction (PCR) amplified polymorphisms. ELECTROPHORESIS. 1995 Jan 1;16(1):1559-1567. https://doi.org/10.1002/elps.11501601259

Simple protocols for typing forensic biological evidence: Chemiluminescent detection for human DNA quantitation and restriction fragment length polymorphism (RELP) analyses and manual typing of polymerase chain reaction (PCR) amplified polymorphisms

Abstract

Keywords

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