Abstract
Methods for identity testing are described that enable extraction of DNA from biological samples, determination of the quantity of human DNA, and genetic analyses of the materials using restriction fragment length polymorphism (RFLP) typing and/or amplified fragment length polymorphism (AMP‐FLP) typing of PCR products. The salient features of the procedures are simplicity, manual typing, nonradioactive chemiluminescent assays or silver staining for detection, and low cost. Most application‐oriented laboratories involved in forensic and/or paternity testing should be able to implement these procedures.
Original language | English |
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Pages (from-to) | 1559-1567 |
Number of pages | 9 |
Journal | ELECTROPHORESIS |
Volume | 16 |
Issue number | 1 |
DOIs | |
State | Published - 1 Jan 1995 |
Keywords
- Chemiluminescence
- DNA extraction
- DNA quantitation
- Multiplex amplification
- Polymerase chain reaction
- Restriction fragment length polymorphism
- Slot blot