Simple apparatus for polarization sensing of analytes

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We describe a simple device for fluorescence sensing based on an unexpansive light source, a dual photocell and a Watson bridge. The emission is detected from two fluorescent samples, one of which changes intensity in response to the analyte. The emission from these two samples is observed through two orthogonally oriented polarizers and an analyzer polarizer. The latter polarizer is rotated to yield equal intensities from both sides of the dual photocell, as determined by a zero voltage from the Watson bridge. Using this device, we are able to measure fluorescein concentration to an accuracy near 2% at 1 μM fluorescein, and pH values accurate to ±0.02 pH units. We also use this approach with a UV hand lamp and a glucose-sensitive protein to measure glucose concentrations near 2 μM to an accuracy of ±0.1 μM. This approach requires only simple electronics, which can be battery powered. Additionally, the method is generic, and can be applied with any fluorescent sample that displays a change in intensity. One can imagine this approach being used to develop portable point-of-care clinical devices.

Original languageEnglish
Pages (from-to)2351-2358
Number of pages8
JournalOptical Engineering
Volume39
Issue number9
DOIs
StatePublished - 1 Sep 2000

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Photoelectric cells
polarizers
Glucose
photoelectric cells
Polarization
glucose
polarization
Electric lamps
Light sources
Electronic equipment
Fluorescence
Proteins
luminaires
electric batteries
analyzers
light sources
Electric potential
proteins
fluorescence
electric potential

Cite this

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abstract = "We describe a simple device for fluorescence sensing based on an unexpansive light source, a dual photocell and a Watson bridge. The emission is detected from two fluorescent samples, one of which changes intensity in response to the analyte. The emission from these two samples is observed through two orthogonally oriented polarizers and an analyzer polarizer. The latter polarizer is rotated to yield equal intensities from both sides of the dual photocell, as determined by a zero voltage from the Watson bridge. Using this device, we are able to measure fluorescein concentration to an accuracy near 2{\%} at 1 μM fluorescein, and pH values accurate to ±0.02 pH units. We also use this approach with a UV hand lamp and a glucose-sensitive protein to measure glucose concentrations near 2 μM to an accuracy of ±0.1 μM. This approach requires only simple electronics, which can be battery powered. Additionally, the method is generic, and can be applied with any fluorescent sample that displays a change in intensity. One can imagine this approach being used to develop portable point-of-care clinical devices.",
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Simple apparatus for polarization sensing of analytes. / Gryczynski, Zygmunt; Gryczynski, Ignacy; Lakowicz, Joseph R.

In: Optical Engineering, Vol. 39, No. 9, 01.09.2000, p. 2351-2358.

Research output: Contribution to journalArticle

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AU - Gryczynski, Zygmunt

AU - Gryczynski, Ignacy

AU - Lakowicz, Joseph R.

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