Silver nanostructure sensing platform for maximum-contrast fluorescence cell imaging

Kyung Min Lee, Arup Neogi, Purnima Basu Neogi, Minjung Kim, Bongsoo Kim, Rafal Luchowski, Zygmunt Gryczynski, Nils Calander, Tae Youl Choif

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We present herein a silver nanostructure-assisted sensing platform which consists of a combined structure of Ag nanowire (NW) and nanodot (ND) array. Highly enhanced fluorescence from fluorophore is attributed to a strongly coupled optical near-field interaction between proximately located Ag NW and NDs. We obtained enhanced fluorescence intensity with up to 140 folds, as contrasted from background intensity, reaching a theoretical maximum value. On the other hand, fluorescence lifetime was greatly reduced to 0.27 ns (from 2.17 ns for the same fluorophores without nanostructure). This novel platform can be a promising utility for optical imaging and labeling of biological systems with a great sensitivity.

Original languageEnglish
Article number056008
JournalJournal of Biomedical Optics
Volume16
Issue number5
DOIs
StatePublished - 1 May 2011

Fingerprint

Silver
Nanostructures
platforms
Fluorophores
Fluorescence
silver
Imaging techniques
fluorescence
Nanowires
nanowires
cells
Biological systems
Labeling
marking
near fields
life (durability)
sensitivity
interactions

Keywords

  • Ag nanowire
  • Cell imaging
  • Fluorescence
  • Focused ion beam
  • Nanodot

Cite this

Lee, K. M., Neogi, A., Neogi, P. B., Kim, M., Kim, B., Luchowski, R., ... Choif, T. Y. (2011). Silver nanostructure sensing platform for maximum-contrast fluorescence cell imaging. Journal of Biomedical Optics, 16(5), [056008]. https://doi.org/10.1117/1.3579157
Lee, Kyung Min ; Neogi, Arup ; Neogi, Purnima Basu ; Kim, Minjung ; Kim, Bongsoo ; Luchowski, Rafal ; Gryczynski, Zygmunt ; Calander, Nils ; Choif, Tae Youl. / Silver nanostructure sensing platform for maximum-contrast fluorescence cell imaging. In: Journal of Biomedical Optics. 2011 ; Vol. 16, No. 5.
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Lee, KM, Neogi, A, Neogi, PB, Kim, M, Kim, B, Luchowski, R, Gryczynski, Z, Calander, N & Choif, TY 2011, 'Silver nanostructure sensing platform for maximum-contrast fluorescence cell imaging', Journal of Biomedical Optics, vol. 16, no. 5, 056008. https://doi.org/10.1117/1.3579157

Silver nanostructure sensing platform for maximum-contrast fluorescence cell imaging. / Lee, Kyung Min; Neogi, Arup; Neogi, Purnima Basu; Kim, Minjung; Kim, Bongsoo; Luchowski, Rafal; Gryczynski, Zygmunt; Calander, Nils; Choif, Tae Youl.

In: Journal of Biomedical Optics, Vol. 16, No. 5, 056008, 01.05.2011.

Research output: Contribution to journalArticle

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