Silver nanostructure sensing platform for maximum-contrast fluorescence cell imaging

Kyung Min Lee; Arup Neogi; Purnima Basu Neogi; Minjung Kim; Bongsoo Kim; Rafal Luchowski; Zygmunt Gryczynski; Nils Calander; Tae Youl Choif

doi:10.1117/1.3579157

Silver nanostructure sensing platform for maximum-contrast fluorescence cell imaging

Kyung Min Lee, Arup Neogi, Purnima Basu Neogi, Minjung Kim, Bongsoo Kim, Rafal Luchowski, Zygmunt Gryczynski, Nils Calander, Tae Youl Choif

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

We present herein a silver nanostructure-assisted sensing platform which consists of a combined structure of Ag nanowire (NW) and nanodot (ND) array. Highly enhanced fluorescence from fluorophore is attributed to a strongly coupled optical near-field interaction between proximately located Ag NW and NDs. We obtained enhanced fluorescence intensity with up to 140 folds, as contrasted from background intensity, reaching a theoretical maximum value. On the other hand, fluorescence lifetime was greatly reduced to 0.27 ns (from 2.17 ns for the same fluorophores without nanostructure). This novel platform can be a promising utility for optical imaging and labeling of biological systems with a great sensitivity.

Original language	English
Article number	056008
Journal	Journal of Biomedical Optics
Volume	16
Issue number	5
DOIs	https://doi.org/10.1117/1.3579157
State	Published - May 2011

Keywords

Ag nanowire
Cell imaging
Fluorescence
Focused ion beam
Nanodot

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title = "Silver nanostructure sensing platform for maximum-contrast fluorescence cell imaging",

abstract = "We present herein a silver nanostructure-assisted sensing platform which consists of a combined structure of Ag nanowire (NW) and nanodot (ND) array. Highly enhanced fluorescence from fluorophore is attributed to a strongly coupled optical near-field interaction between proximately located Ag NW and NDs. We obtained enhanced fluorescence intensity with up to 140 folds, as contrasted from background intensity, reaching a theoretical maximum value. On the other hand, fluorescence lifetime was greatly reduced to 0.27 ns (from 2.17 ns for the same fluorophores without nanostructure). This novel platform can be a promising utility for optical imaging and labeling of biological systems with a great sensitivity.",

keywords = "Ag nanowire, Cell imaging, Fluorescence, Focused ion beam, Nanodot",

author = "Lee, {Kyung Min} and Arup Neogi and Neogi, {Purnima Basu} and Minjung Kim and Bongsoo Kim and Rafal Luchowski and Zygmunt Gryczynski and Nils Calander and Choif, {Tae Youl}",

note = "Funding Information: This project was supported by National Science Foundation (CMMI-0841265) and partially supported by the Center for Nanoscale Mechatronics and Manufacturing of the 21st Century Frontier Research Program. The authors thank the Center for Advanced Research and Technology (CART) at the University of North Texas for supporting this research.",

year = "2011",

month = may,

doi = "10.1117/1.3579157",

language = "English",

volume = "16",

journal = "Journal of Biomedical Optics",

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T1 - Silver nanostructure sensing platform for maximum-contrast fluorescence cell imaging

AU - Lee, Kyung Min

AU - Neogi, Arup

AU - Neogi, Purnima Basu

AU - Kim, Minjung

AU - Kim, Bongsoo

AU - Luchowski, Rafal

AU - Gryczynski, Zygmunt

AU - Calander, Nils

AU - Choif, Tae Youl

N1 - Funding Information: This project was supported by National Science Foundation (CMMI-0841265) and partially supported by the Center for Nanoscale Mechatronics and Manufacturing of the 21st Century Frontier Research Program. The authors thank the Center for Advanced Research and Technology (CART) at the University of North Texas for supporting this research.

PY - 2011/5

Y1 - 2011/5

N2 - We present herein a silver nanostructure-assisted sensing platform which consists of a combined structure of Ag nanowire (NW) and nanodot (ND) array. Highly enhanced fluorescence from fluorophore is attributed to a strongly coupled optical near-field interaction between proximately located Ag NW and NDs. We obtained enhanced fluorescence intensity with up to 140 folds, as contrasted from background intensity, reaching a theoretical maximum value. On the other hand, fluorescence lifetime was greatly reduced to 0.27 ns (from 2.17 ns for the same fluorophores without nanostructure). This novel platform can be a promising utility for optical imaging and labeling of biological systems with a great sensitivity.

AB - We present herein a silver nanostructure-assisted sensing platform which consists of a combined structure of Ag nanowire (NW) and nanodot (ND) array. Highly enhanced fluorescence from fluorophore is attributed to a strongly coupled optical near-field interaction between proximately located Ag NW and NDs. We obtained enhanced fluorescence intensity with up to 140 folds, as contrasted from background intensity, reaching a theoretical maximum value. On the other hand, fluorescence lifetime was greatly reduced to 0.27 ns (from 2.17 ns for the same fluorophores without nanostructure). This novel platform can be a promising utility for optical imaging and labeling of biological systems with a great sensitivity.

KW - Ag nanowire

KW - Cell imaging

KW - Fluorescence

KW - Focused ion beam

KW - Nanodot

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DO - 10.1117/1.3579157

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JO - Journal of Biomedical Optics

JF - Journal of Biomedical Optics

IS - 5

M1 - 056008

ER -

Silver nanostructure sensing platform for maximum-contrast fluorescence cell imaging

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Keywords

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