TY - JOUR
T1 - Silver Fractal-like Structures for Metal-Enhanced Fluorescence
T2 - Enhanced Fluorescence Intensities and Increased Probe Photostabilities
AU - Geddes, Chris D.
AU - Parfenov, Alexandr
AU - Roll, David
AU - Gryczynski, Ignacy
AU - Malicka, Joanna
AU - Lakowicz, Joseph R.
N1 - Funding Information:
This work was supported by the National Institute of Biomedical Imaging and Bioengineering NIH-EB00682 and the National Center for Research Resource, RR-08119.
PY - 2003/5
Y1 - 2003/5
N2 - Substantial increases in fluorescence emission from fluorophore-protein-coated fractal-like silver structures have been observed. We review two methods for silver fractal structure preparation, which have been employed and studied. The first, a roughened silver electrode, typically yielded a 100-fold increase in fluorophore emission, and the second, silver fractal-like structures grown on glass between two silver electrodes, produced a ≈500-fold increase. In addition, significant increases in probe photostability were observed for probes coated on the silver fractal like structures. These results further serve to compliment our recent work on the effects of nobel metal particles with fluorophores, a relatively new phenomenon in fluorescence we have termed both "metal-enhanced fluorescence" and "radiative decay engineering", These results are explained by the metallic surfaces modifying the radiative decay rate (Γ) of the fluorescent labels. We believe that this new silver-surface preparation, which results in ultrabright and photostable fluorophores, offers a new generic technology platform for increased fluorescence signal levels, with widespread potential applications to the analytical sciences, imaging, and medical diagnostics.
AB - Substantial increases in fluorescence emission from fluorophore-protein-coated fractal-like silver structures have been observed. We review two methods for silver fractal structure preparation, which have been employed and studied. The first, a roughened silver electrode, typically yielded a 100-fold increase in fluorophore emission, and the second, silver fractal-like structures grown on glass between two silver electrodes, produced a ≈500-fold increase. In addition, significant increases in probe photostability were observed for probes coated on the silver fractal like structures. These results further serve to compliment our recent work on the effects of nobel metal particles with fluorophores, a relatively new phenomenon in fluorescence we have termed both "metal-enhanced fluorescence" and "radiative decay engineering", These results are explained by the metallic surfaces modifying the radiative decay rate (Γ) of the fluorescent labels. We believe that this new silver-surface preparation, which results in ultrabright and photostable fluorophores, offers a new generic technology platform for increased fluorescence signal levels, with widespread potential applications to the analytical sciences, imaging, and medical diagnostics.
KW - Fractal silver structures
KW - Increased photostabilities
KW - Metal-enhanced fluorescence
KW - Radiative decay engineering
UR - http://www.scopus.com/inward/record.url?scp=0347720890&partnerID=8YFLogxK
U2 - 10.1023/A:1025046101335
DO - 10.1023/A:1025046101335
M3 - Review article
AN - SCOPUS:0347720890
SN - 1053-0509
VL - 13
SP - 267
EP - 276
JO - Journal of Fluorescence
JF - Journal of Fluorescence
IS - 3
ER -