TY - JOUR
T1 - Significant differences in global genomic DNA methylation by gender and race/ethnicity in peripheral blood
AU - Zhang, Fang Fang
AU - Cardarelli, Roberto
AU - Carroll, Joan
AU - Fulda, Kimberly G.
AU - Kaur, Manleen
AU - Gonzalez, Karina
AU - Vishwanatha, Jamboor K.
AU - Santella, Regina M.
AU - Morabia, Alfredo
N1 - Funding Information:
This research received supported from University of North Texas School of Public Health and Institute for Cancer Research, and Joe and Jessie Crump Fund for Medical Education. This research was also supported by NIH grant P20MD001633 to Roberto Cardarelli and Joan Carroll, and NIH grant ES009089 to Regina M. Santella. The authors declared no conflicts of interest.
PY - 2011/5
Y1 - 2011/5
N2 - Reduced levels of global DNA methylation are associated with genomic instability and are independent predictors of cancer risk. Little is known about the environmental determinants of global DNA methylation in peripheral blood. We examined the association between demographic and lifestyle factors and levels of global leukocyte DNA methylation in 161 cancer-free subjects enrolled in the North Texas Healthy Heart Study aged 45-75 years in 2008. We used in-person interviews for demographics and lifestyle factors, a self-administrated Block food frequency questionnaire for diet, and bioelectrical impedance analysis and CT-scan for body composition. We measured genomic DNA methylation using bisulfite conversion of DNA and pyrosequencing for LINE-1. Body composition measures including body mass index, waist circumference, areas of subcutaneous fat and visceral fat, percent of fat mass and fat-free mass were not associated with global genomic DNA methylation after controlling the effect of age, gender and race/ethnicity. Instead, female gender was significantly associated with a reduced level of global methylation (β = -2.77, 95% CI: -4.33, -1.22). Compared to non-Hispanic whites, non-Hispanic blacks (β = -2.02, 95% CI: -3.55, -0.50) had significantly lower levels of global methylation. No association was found with age, cigarette smoking, alcohol drinking and dietary intake of nutrients in one-carbon metabolism. Global leukocyte DNA methylation differs by gender and race/ethnicity, suggesting these variables need to be taken into consideration in studies of global DNA methylation as an epigenetic marker for cancer.
AB - Reduced levels of global DNA methylation are associated with genomic instability and are independent predictors of cancer risk. Little is known about the environmental determinants of global DNA methylation in peripheral blood. We examined the association between demographic and lifestyle factors and levels of global leukocyte DNA methylation in 161 cancer-free subjects enrolled in the North Texas Healthy Heart Study aged 45-75 years in 2008. We used in-person interviews for demographics and lifestyle factors, a self-administrated Block food frequency questionnaire for diet, and bioelectrical impedance analysis and CT-scan for body composition. We measured genomic DNA methylation using bisulfite conversion of DNA and pyrosequencing for LINE-1. Body composition measures including body mass index, waist circumference, areas of subcutaneous fat and visceral fat, percent of fat mass and fat-free mass were not associated with global genomic DNA methylation after controlling the effect of age, gender and race/ethnicity. Instead, female gender was significantly associated with a reduced level of global methylation (β = -2.77, 95% CI: -4.33, -1.22). Compared to non-Hispanic whites, non-Hispanic blacks (β = -2.02, 95% CI: -3.55, -0.50) had significantly lower levels of global methylation. No association was found with age, cigarette smoking, alcohol drinking and dietary intake of nutrients in one-carbon metabolism. Global leukocyte DNA methylation differs by gender and race/ethnicity, suggesting these variables need to be taken into consideration in studies of global DNA methylation as an epigenetic marker for cancer.
KW - DNA methylation
KW - Gender
KW - Race/ethnicity
UR - http://www.scopus.com/inward/record.url?scp=79957570472&partnerID=8YFLogxK
U2 - 10.4161/epi.6.5.15335
DO - 10.4161/epi.6.5.15335
M3 - Article
C2 - 21739720
AN - SCOPUS:79957570472
SN - 1559-2294
VL - 6
SP - 623
EP - 629
JO - Epigenetics
JF - Epigenetics
IS - 5
ER -