Serum cytokine concentrations do not parallel Mycobacterium tuberculosis - Induced cytokine production in patients with tuberculosis

Ramakrishna Vankayalapati, Benjamin Wizel, Stephen Weis, Peter Klucar, Homayoun Shams, Buka Samten, Peter F. Barnes

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

We measured serum cytokine concentrations and Mycobacterium tuberculosis-stimulated cytokine production by peripheral blood mononuclear cells (PBMCs) obtained from persons infected with M. tuberculosis. Serum interferon-γ (IFN-γ) and interleukin-10 (IL-10) concentrations were elevated in patients with tuberculosis compared with healthy persons who had reactions to tuberculin skin tests, but IL-18 concentrations were not. In contrast, M. tuberculosis-stimulated PBMCs from patients with tuberculosis produced less IFN-γ and IL-18 but similar amounts of IL-10, compared with PBMCs from healthy subjects who had reactions to tuberculin skin tests. Pretreatment of PBMCs from healthy subjects with reaction to tuberculin with serum from patients with tuberculosis inhibited IFN-γ production in response to M. tuberculosis, and inhibition was blocked by anti-IL-10. Thus, serum concentrations of IFN-γ, IL-18, and IL-10 do not parallel M. tuberculosis-induced cytokine levels, and increased IL-10 serum levels in patients with tuberculosis inhibit IFN-γ production in response to mycobacterial antigens.

Original languageEnglish
Pages (from-to)24-28
Number of pages5
JournalClinical Infectious Diseases
Volume36
Issue number1
DOIs
StatePublished - 1 Jan 2003

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Mycobacterium tuberculosis
Interleukin-10
Interferons
Tuberculosis
Interleukin-18
Cytokines
Blood Cells
Serum
Tuberculin Test
Skin Tests
Healthy Volunteers
Tuberculin
Antigens

Cite this

Vankayalapati, Ramakrishna ; Wizel, Benjamin ; Weis, Stephen ; Klucar, Peter ; Shams, Homayoun ; Samten, Buka ; Barnes, Peter F. / Serum cytokine concentrations do not parallel Mycobacterium tuberculosis - Induced cytokine production in patients with tuberculosis. In: Clinical Infectious Diseases. 2003 ; Vol. 36, No. 1. pp. 24-28.
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Serum cytokine concentrations do not parallel Mycobacterium tuberculosis - Induced cytokine production in patients with tuberculosis. / Vankayalapati, Ramakrishna; Wizel, Benjamin; Weis, Stephen; Klucar, Peter; Shams, Homayoun; Samten, Buka; Barnes, Peter F.

In: Clinical Infectious Diseases, Vol. 36, No. 1, 01.01.2003, p. 24-28.

Research output: Contribution to journalArticle

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