TY - JOUR
T1 - Serum cytokine concentrations do not parallel Mycobacterium tuberculosis - Induced cytokine production in patients with tuberculosis
AU - Vankayalapati, Ramakrishna
AU - Wizel, Benjamin
AU - Weis, Stephen E.
AU - Klucar, Peter
AU - Shams, Homayoun
AU - Samten, Buka
AU - Barnes, Peter F.
N1 - Funding Information:
Financial support: National Institutes of Health (grant AI-27285), Center for Pulmonary and Infectious Disease Control, the Cain Foundation for Infectious Disease Research, and the Potts Memorial Foundation. P.F.B. holds the Margaret E. Byers Cain Chair for Tuberculosis Research.
PY - 2003/1/1
Y1 - 2003/1/1
N2 - We measured serum cytokine concentrations and Mycobacterium tuberculosis-stimulated cytokine production by peripheral blood mononuclear cells (PBMCs) obtained from persons infected with M. tuberculosis. Serum interferon-γ (IFN-γ) and interleukin-10 (IL-10) concentrations were elevated in patients with tuberculosis compared with healthy persons who had reactions to tuberculin skin tests, but IL-18 concentrations were not. In contrast, M. tuberculosis-stimulated PBMCs from patients with tuberculosis produced less IFN-γ and IL-18 but similar amounts of IL-10, compared with PBMCs from healthy subjects who had reactions to tuberculin skin tests. Pretreatment of PBMCs from healthy subjects with reaction to tuberculin with serum from patients with tuberculosis inhibited IFN-γ production in response to M. tuberculosis, and inhibition was blocked by anti-IL-10. Thus, serum concentrations of IFN-γ, IL-18, and IL-10 do not parallel M. tuberculosis-induced cytokine levels, and increased IL-10 serum levels in patients with tuberculosis inhibit IFN-γ production in response to mycobacterial antigens.
AB - We measured serum cytokine concentrations and Mycobacterium tuberculosis-stimulated cytokine production by peripheral blood mononuclear cells (PBMCs) obtained from persons infected with M. tuberculosis. Serum interferon-γ (IFN-γ) and interleukin-10 (IL-10) concentrations were elevated in patients with tuberculosis compared with healthy persons who had reactions to tuberculin skin tests, but IL-18 concentrations were not. In contrast, M. tuberculosis-stimulated PBMCs from patients with tuberculosis produced less IFN-γ and IL-18 but similar amounts of IL-10, compared with PBMCs from healthy subjects who had reactions to tuberculin skin tests. Pretreatment of PBMCs from healthy subjects with reaction to tuberculin with serum from patients with tuberculosis inhibited IFN-γ production in response to M. tuberculosis, and inhibition was blocked by anti-IL-10. Thus, serum concentrations of IFN-γ, IL-18, and IL-10 do not parallel M. tuberculosis-induced cytokine levels, and increased IL-10 serum levels in patients with tuberculosis inhibit IFN-γ production in response to mycobacterial antigens.
UR - http://www.scopus.com/inward/record.url?scp=0037236693&partnerID=8YFLogxK
U2 - 10.1086/344903
DO - 10.1086/344903
M3 - Article
C2 - 12491197
AN - SCOPUS:0037236693
VL - 36
SP - 24
EP - 28
JO - Clinical Infectious Diseases
JF - Clinical Infectious Diseases
SN - 1058-4838
IS - 1
ER -