Sequential multiplex amplification (SMA) of genetic loci: A method for recovering template DNA for subsequent analyses of additional loci

Miguel Lorente; Jose A. Lorente; Mark R. Wilson; B. Budowle; E. Villanueva

doi:10.1007/BF01225604

Sequential multiplex amplification (SMA) of genetic loci: A method for recovering template DNA for subsequent analyses of additional loci

Miguel Lorente, Jose A. Lorente, Mark R. Wilson, B. Budowle, E. Villanueva

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A method called Sequential Multiplex Amplification (SMA) has been developed whereby a limited amount of DNA extracted from a sample can be reutilized for several single polymerase chain reaction (PCR) amplifications. The method involves recovery of genomic template DNA by microfiltration of PCR-amplified samples. Up to 5 different loci have been typed, each in a single system PCR-based assay, beginning with a test quantity of 5 ng template DNA. Genotypes of the DNA donors were compared with those obtained from individual amplifications and shown to be identical. This could be a useful technique for typing a number of loci from a limited amount of DNa and to recover template DNA from samples previously subjected to PCR. Obviously, when small quantities of template DNA are available, this technique can prove quite useful.

Original language	English
Pages (from-to)	156-158
Number of pages	3
Journal	International journal of legal medicine
Volume	107
Issue number	3
DOIs	https://doi.org/10.1007/BF01225604
State	Published - 1 May 1994

Keywords

DNA
Forensic
PCR
STR
Sequential multiplex amplification (SMA)

Access to Document

10.1007/BF01225604

Cite this

@article{5174762d3e66412788949d32d29b1172,

title = "Sequential multiplex amplification (SMA) of genetic loci: A method for recovering template DNA for subsequent analyses of additional loci",

abstract = "A method called Sequential Multiplex Amplification (SMA) has been developed whereby a limited amount of DNA extracted from a sample can be reutilized for several single polymerase chain reaction (PCR) amplifications. The method involves recovery of genomic template DNA by microfiltration of PCR-amplified samples. Up to 5 different loci have been typed, each in a single system PCR-based assay, beginning with a test quantity of 5 ng template DNA. Genotypes of the DNA donors were compared with those obtained from individual amplifications and shown to be identical. This could be a useful technique for typing a number of loci from a limited amount of DNa and to recover template DNA from samples previously subjected to PCR. Obviously, when small quantities of template DNA are available, this technique can prove quite useful.",

keywords = "DNA, Forensic, PCR, STR, Sequential multiplex amplification (SMA)",

author = "Miguel Lorente and Lorente, {Jose A.} and Wilson, {Mark R.} and B. Budowle and E. Villanueva",

year = "1994",

month = may,

day = "1",

doi = "10.1007/BF01225604",

language = "English",

volume = "107",

pages = "156--158",

journal = "International Journal of Legal Medicine",

issn = "0937-9827",

publisher = "Springer Verlag",

number = "3",

}

TY - JOUR

T1 - Sequential multiplex amplification (SMA) of genetic loci

T2 - A method for recovering template DNA for subsequent analyses of additional loci

AU - Lorente, Miguel

AU - Lorente, Jose A.

AU - Wilson, Mark R.

AU - Budowle, B.

AU - Villanueva, E.

PY - 1994/5/1

Y1 - 1994/5/1

N2 - A method called Sequential Multiplex Amplification (SMA) has been developed whereby a limited amount of DNA extracted from a sample can be reutilized for several single polymerase chain reaction (PCR) amplifications. The method involves recovery of genomic template DNA by microfiltration of PCR-amplified samples. Up to 5 different loci have been typed, each in a single system PCR-based assay, beginning with a test quantity of 5 ng template DNA. Genotypes of the DNA donors were compared with those obtained from individual amplifications and shown to be identical. This could be a useful technique for typing a number of loci from a limited amount of DNa and to recover template DNA from samples previously subjected to PCR. Obviously, when small quantities of template DNA are available, this technique can prove quite useful.

AB - A method called Sequential Multiplex Amplification (SMA) has been developed whereby a limited amount of DNA extracted from a sample can be reutilized for several single polymerase chain reaction (PCR) amplifications. The method involves recovery of genomic template DNA by microfiltration of PCR-amplified samples. Up to 5 different loci have been typed, each in a single system PCR-based assay, beginning with a test quantity of 5 ng template DNA. Genotypes of the DNA donors were compared with those obtained from individual amplifications and shown to be identical. This could be a useful technique for typing a number of loci from a limited amount of DNa and to recover template DNA from samples previously subjected to PCR. Obviously, when small quantities of template DNA are available, this technique can prove quite useful.

KW - DNA

KW - Forensic

KW - PCR

KW - STR

KW - Sequential multiplex amplification (SMA)

UR - http://www.scopus.com/inward/record.url?scp=0027974076&partnerID=8YFLogxK

U2 - 10.1007/BF01225604

DO - 10.1007/BF01225604

M3 - Article

C2 - 7893612

AN - SCOPUS:0027974076

SN - 0937-9827

VL - 107

SP - 156

EP - 158

JO - International Journal of Legal Medicine

JF - International Journal of Legal Medicine

IS - 3

ER -

Sequential multiplex amplification (SMA) of genetic loci: A method for recovering template DNA for subsequent analyses of additional loci

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this