Sequence polymorphism of the mitochondrial DNA hypervariable regions I and II in 205 Singapore Malays

Hang Yee Wong; June S.W. Tang; Bruce Budowle; Marc W. Allard; Christopher K.C. Syn; Wai Fun Tan-Siew; Shui Tse Chow

doi:10.1016/j.legalmed.2006.08.007

Sequence polymorphism of the mitochondrial DNA hypervariable regions I and II in 205 Singapore Malays

Hang Yee Wong, June S.W. Tang, Bruce Budowle, Marc W. Allard, Christopher K.C. Syn, Wai Fun Tan-Siew, Shui Tse Chow

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14 Scopus citations

Abstract

Mitochondrial DNA sequences of the hypervariable regions HV1 and HV2 were analyzed in 205 unrelated ethnic Malays residing in Singapore as an initial effort to generate a database for forensic identification purposes. Sequence polymorphism was detected using PCR and direct sequencing analysis. A total of 152 haplotypes was found containing 152 polymorphisms. Out of the 152 haplotypes, 115 were observed only once and 37 types were seen in multiple individuals. The most common haplotype (16223T, 16295T, 16362C, 73G, 146C, 199C, 263G, and 315.1C) was shared by 7 (3.41%) individuals, two haplotypes were shared by 4 individuals, seven haplotypes were shared by 3 individuals, and 27 haplotypes by 2 individuals. Haplotype diversity and random match probability were estimated to be 0.9961% and 0.87%, respectively. Crown

Original language	English
Pages (from-to)	33-37
Number of pages	5
Journal	Legal Medicine
Volume	9
Issue number	1
DOIs	https://doi.org/10.1016/j.legalmed.2006.08.007
State	Published - 1 Jan 2007

Keywords

Haplogroups
Haplotypes
Heteroplasmy
Sequence polymorphism
Singapore Malay population
mtDNA

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title = "Sequence polymorphism of the mitochondrial DNA hypervariable regions I and II in 205 Singapore Malays",

abstract = "Mitochondrial DNA sequences of the hypervariable regions HV1 and HV2 were analyzed in 205 unrelated ethnic Malays residing in Singapore as an initial effort to generate a database for forensic identification purposes. Sequence polymorphism was detected using PCR and direct sequencing analysis. A total of 152 haplotypes was found containing 152 polymorphisms. Out of the 152 haplotypes, 115 were observed only once and 37 types were seen in multiple individuals. The most common haplotype (16223T, 16295T, 16362C, 73G, 146C, 199C, 263G, and 315.1C) was shared by 7 (3.41%) individuals, two haplotypes were shared by 4 individuals, seven haplotypes were shared by 3 individuals, and 27 haplotypes by 2 individuals. Haplotype diversity and random match probability were estimated to be 0.9961% and 0.87%, respectively. Crown",

keywords = "Haplogroups, Haplotypes, Heteroplasmy, Sequence polymorphism, Singapore Malay population, mtDNA",

author = "Wong, {Hang Yee} and Tang, {June S.W.} and Bruce Budowle and Allard, {Marc W.} and Syn, {Christopher K.C.} and Tan-Siew, {Wai Fun} and Chow, {Shui Tse}",

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AU - Wong, Hang Yee

AU - Tang, June S.W.

AU - Budowle, Bruce

AU - Allard, Marc W.

AU - Syn, Christopher K.C.

AU - Tan-Siew, Wai Fun

AU - Chow, Shui Tse

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N2 - Mitochondrial DNA sequences of the hypervariable regions HV1 and HV2 were analyzed in 205 unrelated ethnic Malays residing in Singapore as an initial effort to generate a database for forensic identification purposes. Sequence polymorphism was detected using PCR and direct sequencing analysis. A total of 152 haplotypes was found containing 152 polymorphisms. Out of the 152 haplotypes, 115 were observed only once and 37 types were seen in multiple individuals. The most common haplotype (16223T, 16295T, 16362C, 73G, 146C, 199C, 263G, and 315.1C) was shared by 7 (3.41%) individuals, two haplotypes were shared by 4 individuals, seven haplotypes were shared by 3 individuals, and 27 haplotypes by 2 individuals. Haplotype diversity and random match probability were estimated to be 0.9961% and 0.87%, respectively. Crown

AB - Mitochondrial DNA sequences of the hypervariable regions HV1 and HV2 were analyzed in 205 unrelated ethnic Malays residing in Singapore as an initial effort to generate a database for forensic identification purposes. Sequence polymorphism was detected using PCR and direct sequencing analysis. A total of 152 haplotypes was found containing 152 polymorphisms. Out of the 152 haplotypes, 115 were observed only once and 37 types were seen in multiple individuals. The most common haplotype (16223T, 16295T, 16362C, 73G, 146C, 199C, 263G, and 315.1C) was shared by 7 (3.41%) individuals, two haplotypes were shared by 4 individuals, seven haplotypes were shared by 3 individuals, and 27 haplotypes by 2 individuals. Haplotype diversity and random match probability were estimated to be 0.9961% and 0.87%, respectively. Crown

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KW - Haplotypes

KW - Heteroplasmy

KW - Sequence polymorphism

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