Sequence polymorphism of the mitochondrial DNA hypervariable regions I and II in 205 Singapore Malays

Hang Yee Wong, June S.W. Tang, Bruce Budowle, Marc W. Allard, Christopher K.C. Syn, Wai Fun Tan-Siew, Shui Tse Chow

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Mitochondrial DNA sequences of the hypervariable regions HV1 and HV2 were analyzed in 205 unrelated ethnic Malays residing in Singapore as an initial effort to generate a database for forensic identification purposes. Sequence polymorphism was detected using PCR and direct sequencing analysis. A total of 152 haplotypes was found containing 152 polymorphisms. Out of the 152 haplotypes, 115 were observed only once and 37 types were seen in multiple individuals. The most common haplotype (16223T, 16295T, 16362C, 73G, 146C, 199C, 263G, and 315.1C) was shared by 7 (3.41%) individuals, two haplotypes were shared by 4 individuals, seven haplotypes were shared by 3 individuals, and 27 haplotypes by 2 individuals. Haplotype diversity and random match probability were estimated to be 0.9961% and 0.87%, respectively. Crown

Original languageEnglish
Pages (from-to)33-37
Number of pages5
JournalLegal Medicine
Volume9
Issue number1
DOIs
StatePublished - 1 Jan 2007

Keywords

  • Haplogroups
  • Haplotypes
  • Heteroplasmy
  • Sequence polymorphism
  • Singapore Malay population
  • mtDNA

Fingerprint Dive into the research topics of 'Sequence polymorphism of the mitochondrial DNA hypervariable regions I and II in 205 Singapore Malays'. Together they form a unique fingerprint.

Cite this