Separation of dansylated 17β-estradiol, 17α-estradiol, and estrone on a single HPLC column for simultaneous quantitation by LC-MS/MS

Szabolcs Szarka, Vien Nguyen, Laszlo Prokai, Katalin Prokai-Tatrai

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

We show here that baseline separation of dansylated estrone, 17β-estradiol, and 17α-estradiol can be done, contrary to previous reports, within a short run time on a single RP-LC analytical column packed with particles bonded with phenyl-hexyl stationary phase. The chromatographic method coupled with isotope dilution tandem MS offers a simple assay enabling the simultaneous analysis of these analytes. The method employs 13C- labeled estrogens as internal standards to eliminate potential matrix effects arising from the use of deuterated estrogens. The assay also offers adequate accuracy and sensitivity to be useful for biological samples. The practical applicability of the validated method is demonstrated by the quantitative analyses of in vivo samples obtained from rats treated with Premarin®. [Figure not available: see fulltext.]

Original languageEnglish
Pages (from-to)3399-3406
Number of pages8
JournalAnalytical and bioanalytical chemistry
Volume405
Issue number10
DOIs
StatePublished - Apr 2013

Keywords

  • C-labeled internal standards
  • Dansylation
  • Estrogens
  • LC-MS/MS
  • Phenyl-hexyl stationary phase

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