Separation and detection of lipoproteins in human serum by use of size-exclusion liquid chromatography: A preliminary report

D. L. Busbee; D. M. Payne; D. W. Jasheway; S. Carlisle; A. G. Lacko

doi:10.1093/clinchem/27.12.2052

Separation and detection of lipoproteins in human serum by use of size-exclusion liquid chromatography: A preliminary report

D. L. Busbee, D. M. Payne, D. W. Jasheway, S. Carlisle, A. G. Lacko

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Components of human serum can be separated on the basis of differences in relative molecular mass by using size-exclusion 'high-performance' liquid chromatography. Lipoproteins in fractions of the eluate can be quantitated by conventional chemical and enzymatic methods. Alternatively, if lipoproteins in the serum are selectively presented with diformazan dye, the column effluent can be monitored spectrophotometrically at 580 nm, so that only the lipoprotein components of serum are detected. Samples of purified low-density lipoproteins, so stained and analyzed, provide peak-area values that are proportional to their concentration as evaluated by chemical methods. With this technique, the various lipoprotein classes can be quickly separated and their concentration estimated. These techniques should prove useful in clinical and research laboratories.

Original language	English
Pages (from-to)	2052-2058
Number of pages	7
Journal	Unknown Journal
Volume	27
Issue number	12
DOIs	https://doi.org/10.1093/clinchem/27.12.2052
State	Published - 1981

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10.1093/clinchem/27.12.2052

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abstract = "Components of human serum can be separated on the basis of differences in relative molecular mass by using size-exclusion 'high-performance' liquid chromatography. Lipoproteins in fractions of the eluate can be quantitated by conventional chemical and enzymatic methods. Alternatively, if lipoproteins in the serum are selectively presented with diformazan dye, the column effluent can be monitored spectrophotometrically at 580 nm, so that only the lipoprotein components of serum are detected. Samples of purified low-density lipoproteins, so stained and analyzed, provide peak-area values that are proportional to their concentration as evaluated by chemical methods. With this technique, the various lipoprotein classes can be quickly separated and their concentration estimated. These techniques should prove useful in clinical and research laboratories.",

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T1 - Separation and detection of lipoproteins in human serum by use of size-exclusion liquid chromatography

T2 - A preliminary report

AU - Busbee, D. L.

AU - Payne, D. M.

AU - Jasheway, D. W.

AU - Carlisle, S.

AU - Lacko, A. G.

PY - 1981

Y1 - 1981

N2 - Components of human serum can be separated on the basis of differences in relative molecular mass by using size-exclusion 'high-performance' liquid chromatography. Lipoproteins in fractions of the eluate can be quantitated by conventional chemical and enzymatic methods. Alternatively, if lipoproteins in the serum are selectively presented with diformazan dye, the column effluent can be monitored spectrophotometrically at 580 nm, so that only the lipoprotein components of serum are detected. Samples of purified low-density lipoproteins, so stained and analyzed, provide peak-area values that are proportional to their concentration as evaluated by chemical methods. With this technique, the various lipoprotein classes can be quickly separated and their concentration estimated. These techniques should prove useful in clinical and research laboratories.

AB - Components of human serum can be separated on the basis of differences in relative molecular mass by using size-exclusion 'high-performance' liquid chromatography. Lipoproteins in fractions of the eluate can be quantitated by conventional chemical and enzymatic methods. Alternatively, if lipoproteins in the serum are selectively presented with diformazan dye, the column effluent can be monitored spectrophotometrically at 580 nm, so that only the lipoprotein components of serum are detected. Samples of purified low-density lipoproteins, so stained and analyzed, provide peak-area values that are proportional to their concentration as evaluated by chemical methods. With this technique, the various lipoprotein classes can be quickly separated and their concentration estimated. These techniques should prove useful in clinical and research laboratories.

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DO - 10.1093/clinchem/27.12.2052

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C2 - 6171365

AN - SCOPUS:0019783179

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SP - 2052

EP - 2058

JO - Unknown Journal

JF - Unknown Journal

IS - 12

ER -

Separation and detection of lipoproteins in human serum by use of size-exclusion liquid chromatography: A preliminary report

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