Self-quenching of uranin: Instrument response function for color sensitive photo-detectors

Rafal Luchowski, Sushant Sabnis, Mariusz Szabelski, Pabak Sarkar, Sangram Limbaji Raut, Zygmunt Gryczynski, Julian Borejdo, Piotr Bojarski, Ignacy Gryczynski

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Concentration is a key determining factor in the fluorescence properties of organic fluorophores. We studied self-quenching of disodium fluorescein (uranin) fluorescence in polyvinyl alcohol (PVA) thin films. The concentration dependent changes in brightness and anisotropy were followed by a lifetime decrease. We found that at a concentration of 0.54 M, the lifetime decreases to 7 ps. At a concentration of 0.18 M the lifetime was 10 ps with the relatively high quantum yield of 0.002. In these conditions the fluorescence intensity decay was homogeneous (well approximated by a single lifetime). We realized that such a sample was an ideal fluorescence lifetime standard for spectroscopy and microscopy, and therefore characterized instrument response functions for a time-domain technique. We show that self-quenched uranin enables measurements free of the color effect, making it a superior choice for a lifetime reference over scattered light.

Original languageEnglish
Pages (from-to)2446-2451
Number of pages6
JournalJournal of Luminescence
Volume130
Issue number12
DOIs
StatePublished - 1 Dec 2010

Fingerprint

Quenching
Color
Fluorescence
quenching
Detectors
color
life (durability)
detectors
fluorescence
Polyvinyl Alcohol
Fluorophores
Anisotropy
Quantum yield
Fluorescein
Microscopy
Luminance
Spectrum Analysis
Microscopic examination
polyvinyl alcohol
Spectroscopy

Keywords

  • Homo-FRET
  • Homotransfer
  • Instrument response function
  • Lifetime standard
  • Self-quenching
  • Single photon avalanche diode

Cite this

Luchowski, Rafal ; Sabnis, Sushant ; Szabelski, Mariusz ; Sarkar, Pabak ; Raut, Sangram Limbaji ; Gryczynski, Zygmunt ; Borejdo, Julian ; Bojarski, Piotr ; Gryczynski, Ignacy. / Self-quenching of uranin : Instrument response function for color sensitive photo-detectors. In: Journal of Luminescence. 2010 ; Vol. 130, No. 12. pp. 2446-2451.
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Self-quenching of uranin : Instrument response function for color sensitive photo-detectors. / Luchowski, Rafal; Sabnis, Sushant; Szabelski, Mariusz; Sarkar, Pabak; Raut, Sangram Limbaji; Gryczynski, Zygmunt; Borejdo, Julian; Bojarski, Piotr; Gryczynski, Ignacy.

In: Journal of Luminescence, Vol. 130, No. 12, 01.12.2010, p. 2446-2451.

Research output: Contribution to journalArticle

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T1 - Self-quenching of uranin

T2 - Instrument response function for color sensitive photo-detectors

AU - Luchowski, Rafal

AU - Sabnis, Sushant

AU - Szabelski, Mariusz

AU - Sarkar, Pabak

AU - Raut, Sangram Limbaji

AU - Gryczynski, Zygmunt

AU - Borejdo, Julian

AU - Bojarski, Piotr

AU - Gryczynski, Ignacy

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AB - Concentration is a key determining factor in the fluorescence properties of organic fluorophores. We studied self-quenching of disodium fluorescein (uranin) fluorescence in polyvinyl alcohol (PVA) thin films. The concentration dependent changes in brightness and anisotropy were followed by a lifetime decrease. We found that at a concentration of 0.54 M, the lifetime decreases to 7 ps. At a concentration of 0.18 M the lifetime was 10 ps with the relatively high quantum yield of 0.002. In these conditions the fluorescence intensity decay was homogeneous (well approximated by a single lifetime). We realized that such a sample was an ideal fluorescence lifetime standard for spectroscopy and microscopy, and therefore characterized instrument response functions for a time-domain technique. We show that self-quenched uranin enables measurements free of the color effect, making it a superior choice for a lifetime reference over scattered light.

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