Selective extraction and effective separation of galactosylsphingosine (Psychosine) and glucosylsphingosine from other glycosphingolipids in pathological tissue samples

Yu Teh Li; Su Chen Li; Wayne R. Buck; Mark E. Haskins; Sz Wei Wu; Kay Hooi Khoo; Ellen Sidransky; Bruce A. Bunnell

doi:10.1007/s11064-010-0348-3

Selective extraction and effective separation of galactosylsphingosine (Psychosine) and glucosylsphingosine from other glycosphingolipids in pathological tissue samples

Yu Teh Li, Su Chen Li, Wayne R. Buck, Mark E. Haskins, Sz Wei Wu, Kay Hooi Khoo, Ellen Sidransky, Bruce A. Bunnell

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

To facilitate the study of the chemical pathology of galactosylsphingosine (psychosine, GalSph) in Krabbe disease and glucosylsphingosine (GlcSph) in Gaucher disease, we have devised a facile method for the effective separation of these two glycosylsphingosines from other glycosphingolipids (GSLs) in Krabbe brain and Gaucher spleen samples. The procedure involves the use of acetone to selectively extract GalSph and GlcSph, respectively, from Krabbe brain and Gaucher spleen samples. Since acetone does not extract other GSLs except modest amounts of galactosylceramide, sulfatide, and glucosylceramide, the positively charged GalSph or GlcSph in the acetone extract can be readily separated from other GSLs by batchwise cation-exchange chromatography using a Waters Accell Plus CM Cartridge. GalSph or GlcSph enriched by this simple procedure can be readily analyzed by thin-layer chromatography or high-performance liquid chromatography.

Original language	English
Pages (from-to)	1612-1622
Number of pages	11
Journal	Neurochemical Research
Volume	36
Issue number	9
DOIs	https://doi.org/10.1007/s11064-010-0348-3
State	Published - Sep 2011

Keywords

Galactosylsphingosine
Gaucher disease
Glucosylsphingosine
Krabbe disease
Psychosine

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10.1007/s11064-010-0348-3

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@article{495777b48b62486db12d280c5aae0f37,

title = "Selective extraction and effective separation of galactosylsphingosine (Psychosine) and glucosylsphingosine from other glycosphingolipids in pathological tissue samples",

abstract = "To facilitate the study of the chemical pathology of galactosylsphingosine (psychosine, GalSph) in Krabbe disease and glucosylsphingosine (GlcSph) in Gaucher disease, we have devised a facile method for the effective separation of these two glycosylsphingosines from other glycosphingolipids (GSLs) in Krabbe brain and Gaucher spleen samples. The procedure involves the use of acetone to selectively extract GalSph and GlcSph, respectively, from Krabbe brain and Gaucher spleen samples. Since acetone does not extract other GSLs except modest amounts of galactosylceramide, sulfatide, and glucosylceramide, the positively charged GalSph or GlcSph in the acetone extract can be readily separated from other GSLs by batchwise cation-exchange chromatography using a Waters Accell Plus CM Cartridge. GalSph or GlcSph enriched by this simple procedure can be readily analyzed by thin-layer chromatography or high-performance liquid chromatography.",

keywords = "Galactosylsphingosine, Gaucher disease, Glucosylsphingosine, Krabbe disease, Psychosine",

author = "Li, {Yu Teh} and Li, {Su Chen} and Buck, {Wayne R.} and Haskins, {Mark E.} and Wu, {Sz Wei} and Khoo, {Kay Hooi} and Ellen Sidransky and Bunnell, {Bruce A.}",

note = "Funding Information: Acknowledgments This study was supported by NIH grant R01 NS09626 (to YT Li), NIH grant RR02512 (to ME Haskins), NIH grant NCRR R24RR022826 and Louisiana Gene Therapy Research Consortium Tulane University (to B. Bunnell). The human brain samples from Krabbe patients and the age matched control subjects were obtained from the NICHD Brain and Tissue Bank for Developmental Disorders at the University of Maryland, Baltimore, MD. MALDI-MS analyses were performed at the NRPGM Core Facilities for Proteomics and Glycomics, Institute of Biological Chemistry, Academia Sinica, Taiwan, supported by an NSC grant NSC98-3112-B-001-023.",

year = "2011",

month = sep,

doi = "10.1007/s11064-010-0348-3",

language = "English",

volume = "36",

pages = "1612--1622",

journal = "Neurochemical Research",

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TY - JOUR

T1 - Selective extraction and effective separation of galactosylsphingosine (Psychosine) and glucosylsphingosine from other glycosphingolipids in pathological tissue samples

AU - Li, Yu Teh

AU - Li, Su Chen

AU - Buck, Wayne R.

AU - Haskins, Mark E.

AU - Wu, Sz Wei

AU - Khoo, Kay Hooi

AU - Sidransky, Ellen

AU - Bunnell, Bruce A.

N1 - Funding Information: Acknowledgments This study was supported by NIH grant R01 NS09626 (to YT Li), NIH grant RR02512 (to ME Haskins), NIH grant NCRR R24RR022826 and Louisiana Gene Therapy Research Consortium Tulane University (to B. Bunnell). The human brain samples from Krabbe patients and the age matched control subjects were obtained from the NICHD Brain and Tissue Bank for Developmental Disorders at the University of Maryland, Baltimore, MD. MALDI-MS analyses were performed at the NRPGM Core Facilities for Proteomics and Glycomics, Institute of Biological Chemistry, Academia Sinica, Taiwan, supported by an NSC grant NSC98-3112-B-001-023.

PY - 2011/9

Y1 - 2011/9

N2 - To facilitate the study of the chemical pathology of galactosylsphingosine (psychosine, GalSph) in Krabbe disease and glucosylsphingosine (GlcSph) in Gaucher disease, we have devised a facile method for the effective separation of these two glycosylsphingosines from other glycosphingolipids (GSLs) in Krabbe brain and Gaucher spleen samples. The procedure involves the use of acetone to selectively extract GalSph and GlcSph, respectively, from Krabbe brain and Gaucher spleen samples. Since acetone does not extract other GSLs except modest amounts of galactosylceramide, sulfatide, and glucosylceramide, the positively charged GalSph or GlcSph in the acetone extract can be readily separated from other GSLs by batchwise cation-exchange chromatography using a Waters Accell Plus CM Cartridge. GalSph or GlcSph enriched by this simple procedure can be readily analyzed by thin-layer chromatography or high-performance liquid chromatography.

AB - To facilitate the study of the chemical pathology of galactosylsphingosine (psychosine, GalSph) in Krabbe disease and glucosylsphingosine (GlcSph) in Gaucher disease, we have devised a facile method for the effective separation of these two glycosylsphingosines from other glycosphingolipids (GSLs) in Krabbe brain and Gaucher spleen samples. The procedure involves the use of acetone to selectively extract GalSph and GlcSph, respectively, from Krabbe brain and Gaucher spleen samples. Since acetone does not extract other GSLs except modest amounts of galactosylceramide, sulfatide, and glucosylceramide, the positively charged GalSph or GlcSph in the acetone extract can be readily separated from other GSLs by batchwise cation-exchange chromatography using a Waters Accell Plus CM Cartridge. GalSph or GlcSph enriched by this simple procedure can be readily analyzed by thin-layer chromatography or high-performance liquid chromatography.

KW - Galactosylsphingosine

KW - Gaucher disease

KW - Glucosylsphingosine

KW - Krabbe disease

KW - Psychosine

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U2 - 10.1007/s11064-010-0348-3

DO - 10.1007/s11064-010-0348-3

M3 - Article

C2 - 21136152

AN - SCOPUS:80755136700

SN - 0364-3190

VL - 36

SP - 1612

EP - 1622

JO - Neurochemical Research

JF - Neurochemical Research

IS - 9

ER -

Selective extraction and effective separation of galactosylsphingosine (Psychosine) and glucosylsphingosine from other glycosphingolipids in pathological tissue samples

Abstract

Keywords

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