SDF-1/CXCR7 Chemokine signaling is induced in the peri-infarct regions in patients with ischemic stroke

Yu Zhang, Hongxia Zhang, Siyang Lin, Xudong Chen, Yu Yao, Xiao Ou Mao, Bei Shao, Qichuan Zhuge, Kunlin Jin

Research output: Contribution to journalArticleResearchpeer-review

1 Citation (Scopus)

Abstract

Stromal-derived factor-1 (SDF-1, also known as CXCL12) and its receptors CXCR4 and CXCR7 play important roles in brain repair after ischemic stroke, as SDF-1/CXCR4/CXCR7 chemokine signaling is critical for recruiting stem cells to sites of ischemic injury. Upregulation of SDF-1/CXCR4/CXCR7 chemokine signaling in the ischemic regions has been well-documented in the animal models of ischemic stroke, but not in human ischemic brain. Here, we found that protein expression of SDF-1 and CXCR7, but not CXCR4, were significantly increased in the cortical peri-infarct regions (penumbra) after ischemic stroke in human, compared with adjacent normal tissues and control subjects. Double-label fluorescence immunohistochemistry shows that SDF-1 and CXCR4 proteins were expressed in neuronal cells and astrocytes in the normal brain tissue and peri-infarct regions. CXCR7 protein was also observed in neuronal cells and astrocytes in the normal cortical regions, but predominantly in astrocytes in the penumbra of ischemic brain. Our data suggest that ischemic stroke in human leads to an increase in the expression of SDF-1 and CXCR7, but not CXCR4, in the peri-infarct cerebral cortex. Our findings suggest that chemokine SFD-1 is expressed not only in animal models of stroke, but also in the human brain after an ischemic injury. In addition, unlike animals, CXCR7 may be the primary receptor of SDF-1 in human stroke brain.

Original languageEnglish
Pages (from-to)287-295
Number of pages9
JournalAging and Disease
Volume9
Issue number2
DOIs
StatePublished - 1 Apr 2018

Fingerprint

Chemokines
Stroke
Brain
Astrocytes
Animal Models
CXCR4 Receptors
Proteins
Wounds and Injuries
Cerebral Cortex
Up-Regulation
Stem Cells
Fluorescence
Immunohistochemistry

Keywords

  • CXCL12
  • CXCR4
  • CXCR7
  • Chemokine
  • Human
  • Ischemia
  • Patient
  • Penumbra
  • SDF-1
  • Stroke

Cite this

Zhang, Yu ; Zhang, Hongxia ; Lin, Siyang ; Chen, Xudong ; Yao, Yu ; Mao, Xiao Ou ; Shao, Bei ; Zhuge, Qichuan ; Jin, Kunlin. / SDF-1/CXCR7 Chemokine signaling is induced in the peri-infarct regions in patients with ischemic stroke. In: Aging and Disease. 2018 ; Vol. 9, No. 2. pp. 287-295.
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title = "SDF-1/CXCR7 Chemokine signaling is induced in the peri-infarct regions in patients with ischemic stroke",
abstract = "Stromal-derived factor-1 (SDF-1, also known as CXCL12) and its receptors CXCR4 and CXCR7 play important roles in brain repair after ischemic stroke, as SDF-1/CXCR4/CXCR7 chemokine signaling is critical for recruiting stem cells to sites of ischemic injury. Upregulation of SDF-1/CXCR4/CXCR7 chemokine signaling in the ischemic regions has been well-documented in the animal models of ischemic stroke, but not in human ischemic brain. Here, we found that protein expression of SDF-1 and CXCR7, but not CXCR4, were significantly increased in the cortical peri-infarct regions (penumbra) after ischemic stroke in human, compared with adjacent normal tissues and control subjects. Double-label fluorescence immunohistochemistry shows that SDF-1 and CXCR4 proteins were expressed in neuronal cells and astrocytes in the normal brain tissue and peri-infarct regions. CXCR7 protein was also observed in neuronal cells and astrocytes in the normal cortical regions, but predominantly in astrocytes in the penumbra of ischemic brain. Our data suggest that ischemic stroke in human leads to an increase in the expression of SDF-1 and CXCR7, but not CXCR4, in the peri-infarct cerebral cortex. Our findings suggest that chemokine SFD-1 is expressed not only in animal models of stroke, but also in the human brain after an ischemic injury. In addition, unlike animals, CXCR7 may be the primary receptor of SDF-1 in human stroke brain.",
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author = "Yu Zhang and Hongxia Zhang and Siyang Lin and Xudong Chen and Yu Yao and Mao, {Xiao Ou} and Bei Shao and Qichuan Zhuge and Kunlin Jin",
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Zhang, Y, Zhang, H, Lin, S, Chen, X, Yao, Y, Mao, XO, Shao, B, Zhuge, Q & Jin, K 2018, 'SDF-1/CXCR7 Chemokine signaling is induced in the peri-infarct regions in patients with ischemic stroke', Aging and Disease, vol. 9, no. 2, pp. 287-295. https://doi.org/10.14336/AD.2017.1112

SDF-1/CXCR7 Chemokine signaling is induced in the peri-infarct regions in patients with ischemic stroke. / Zhang, Yu; Zhang, Hongxia; Lin, Siyang; Chen, Xudong; Yao, Yu; Mao, Xiao Ou; Shao, Bei; Zhuge, Qichuan; Jin, Kunlin.

In: Aging and Disease, Vol. 9, No. 2, 01.04.2018, p. 287-295.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Zhang, Yu

AU - Zhang, Hongxia

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AU - Yao, Yu

AU - Mao, Xiao Ou

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AU - Zhuge, Qichuan

AU - Jin, Kunlin

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