TY - JOUR
T1 - Roughened silver electrodes for use in metal-enhanced fluorescence
AU - Geddes, Chris D.
AU - Parfenov, Alexandr
AU - Roll, David
AU - Gryczynski, Ignacy
AU - Malicka, Joanna
AU - Lakowicz, Joseph R.
N1 - Funding Information:
This work was supported by the National Institute of Biomedical Imaging and Bioengineering NIH EB-00682 and the National Center for Research Resource, RR-08119.
PY - 2004/7
Y1 - 2004/7
N2 - Roughened silver electrodes are widely used for surface-enhanced Raman scattering (SERS). We tested roughened silver electrodes for metal-enhanced fluorescence. Constant current between two silver electrodes in pure water resulted in the growth of fractal-like structures on the cathode. This electrode was coated with a monolayer of human serum albumin (HSA) protein that had been labeled with a fluorescent dye, indocyanine green (ICG). The fluorescence intensity of ICG-HSA on the roughened electrode increased by ≈50-fold relative to the unroughened electrode, which was essentially non-fluorescent and increased typically two-fold as compared to the silver anode. No fractal-like structures were observed on the anode. Lifetime measurements showed that at least part of the increased intensity was due to an increased radiative decay rate of ICG. In our opinion, the use of in situ generated roughened silver electrodes will find multifarious applications in analytical chemistry, such as in fluorescence based assays, in an analogous manner to the now widespread use of SERS. To the best of our knowledge this is the first report of roughened silver electrodes for metal-enhanced fluorescence.
AB - Roughened silver electrodes are widely used for surface-enhanced Raman scattering (SERS). We tested roughened silver electrodes for metal-enhanced fluorescence. Constant current between two silver electrodes in pure water resulted in the growth of fractal-like structures on the cathode. This electrode was coated with a monolayer of human serum albumin (HSA) protein that had been labeled with a fluorescent dye, indocyanine green (ICG). The fluorescence intensity of ICG-HSA on the roughened electrode increased by ≈50-fold relative to the unroughened electrode, which was essentially non-fluorescent and increased typically two-fold as compared to the silver anode. No fractal-like structures were observed on the anode. Lifetime measurements showed that at least part of the increased intensity was due to an increased radiative decay rate of ICG. In our opinion, the use of in situ generated roughened silver electrodes will find multifarious applications in analytical chemistry, such as in fluorescence based assays, in an analogous manner to the now widespread use of SERS. To the best of our knowledge this is the first report of roughened silver electrodes for metal-enhanced fluorescence.
KW - Metal-enhanced fluorescence
KW - Roughened silver electrodes
KW - Surface-enhanced Raman scattering
UR - http://www.scopus.com/inward/record.url?scp=3042718290&partnerID=8YFLogxK
U2 - 10.1016/j.saa.2003.10.014
DO - 10.1016/j.saa.2003.10.014
M3 - Article
C2 - 15248975
AN - SCOPUS:3042718290
SN - 1386-1425
VL - 60
SP - 1977
EP - 1983
JO - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
JF - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
IS - 8-9
ER -