Rotations of a few cross-bridges in muscle by confocal total internal reflection microscopy

J. Borejdo, J. Talent, I. Akopova, T. P. Burghardt

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In order to measure the cycling of a few (∼6) myosin heads in contracting skeletal muscle, myofibrils were illuminated by Total Internal Reflection and observed through a confocal aperture. Myosin heads rotated at a rate approximately equal to the ATPase rate, suggesting that bulk ATPase of a whole muscle reflects the cycle frequency of individual heads.

Original languageEnglish
Pages (from-to)137-140
Number of pages4
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1763
Issue number2
DOIs
StatePublished - 1 Feb 2006

Fingerprint

Myosins
Adenosine Triphosphatases
Microscopy
Muscles
Myofibrils
Skeletal Muscle
Head

Keywords

  • Confocal microscopy
  • Cross-bridge
  • Rotational motion
  • Total internal reflection microscopy

Cite this

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Rotations of a few cross-bridges in muscle by confocal total internal reflection microscopy. / Borejdo, J.; Talent, J.; Akopova, I.; Burghardt, T. P.

In: Biochimica et Biophysica Acta - Molecular Cell Research, Vol. 1763, No. 2, 01.02.2006, p. 137-140.

Research output: Contribution to journalArticle

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AU - Akopova, I.

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