Rotations of a few cross-bridges in muscle by confocal total internal reflection microscopy

J. Borejdo; J. Talent; I. Akopova; T. P. Burghardt

doi:10.1016/j.bbamcr.2005.11.011

Rotations of a few cross-bridges in muscle by confocal total internal reflection microscopy

J. Borejdo, J. Talent, I. Akopova, T. P. Burghardt

Microbiology, Immunology & Genetics

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

In order to measure the cycling of a few (∼6) myosin heads in contracting skeletal muscle, myofibrils were illuminated by Total Internal Reflection and observed through a confocal aperture. Myosin heads rotated at a rate approximately equal to the ATPase rate, suggesting that bulk ATPase of a whole muscle reflects the cycle frequency of individual heads.

Original language	English
Pages (from-to)	137-140
Number of pages	4
Journal	Biochimica et Biophysica Acta - Molecular Cell Research
Volume	1763
Issue number	2
DOIs	https://doi.org/10.1016/j.bbamcr.2005.11.011
State	Published - Feb 2006

Keywords

Confocal microscopy
Cross-bridge
Rotational motion
Total internal reflection microscopy

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10.1016/j.bbamcr.2005.11.011

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title = "Rotations of a few cross-bridges in muscle by confocal total internal reflection microscopy",

abstract = "In order to measure the cycling of a few (∼6) myosin heads in contracting skeletal muscle, myofibrils were illuminated by Total Internal Reflection and observed through a confocal aperture. Myosin heads rotated at a rate approximately equal to the ATPase rate, suggesting that bulk ATPase of a whole muscle reflects the cycle frequency of individual heads.",

keywords = "Confocal microscopy, Cross-bridge, Rotational motion, Total internal reflection microscopy",

author = "J. Borejdo and J. Talent and I. Akopova and Burghardt, {T. P.}",

note = "Funding Information: Supported by R21CA9732 and R01AR048622 (NIAMS) (JB) and by R01AR049277 (NIAMS) and the Mayo Foundation (TPB).",

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AU - Borejdo, J.

AU - Talent, J.

AU - Akopova, I.

AU - Burghardt, T. P.

N1 - Funding Information: Supported by R21CA9732 and R01AR048622 (NIAMS) (JB) and by R01AR049277 (NIAMS) and the Mayo Foundation (TPB).

PY - 2006/2

Y1 - 2006/2

N2 - In order to measure the cycling of a few (∼6) myosin heads in contracting skeletal muscle, myofibrils were illuminated by Total Internal Reflection and observed through a confocal aperture. Myosin heads rotated at a rate approximately equal to the ATPase rate, suggesting that bulk ATPase of a whole muscle reflects the cycle frequency of individual heads.

AB - In order to measure the cycling of a few (∼6) myosin heads in contracting skeletal muscle, myofibrils were illuminated by Total Internal Reflection and observed through a confocal aperture. Myosin heads rotated at a rate approximately equal to the ATPase rate, suggesting that bulk ATPase of a whole muscle reflects the cycle frequency of individual heads.

KW - Confocal microscopy

KW - Cross-bridge

KW - Rotational motion

KW - Total internal reflection microscopy

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U2 - 10.1016/j.bbamcr.2005.11.011

DO - 10.1016/j.bbamcr.2005.11.011

M3 - Article

C2 - 16510199

AN - SCOPUS:33644982036

SN - 0167-4889

VL - 1763

SP - 137

EP - 140

JO - BBA - Molecular Cell Research

JF - BBA - Molecular Cell Research

IS - 2

ER -

Rotations of a few cross-bridges in muscle by confocal total internal reflection microscopy

Abstract

Keywords

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