In order to measure the cycling of a few (∼6) myosin heads in contracting skeletal muscle, myofibrils were illuminated by Total Internal Reflection and observed through a confocal aperture. Myosin heads rotated at a rate approximately equal to the ATPase rate, suggesting that bulk ATPase of a whole muscle reflects the cycle frequency of individual heads.
|Number of pages||4|
|Journal||Biochimica et Biophysica Acta - Molecular Cell Research|
|State||Published - 1 Feb 2006|
- Confocal microscopy
- Rotational motion
- Total internal reflection microscopy