Role of Tec1 in the development, architecture, and integrity of sexual biofilms of Candida albicans

Karla J. Daniels, Srikantha Thyagarajan, Claude Pujol, Yang Nim Park, David R. Soll

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9 Citations (Scopus)

Abstract

MTL-homozygous (a/a or a/a) white cells form a complex sexual biofilm that1exhibits the same architecture as that of MTLheterozygous (a/a) pathogenic biofilms. However, the former is regulated by the mitogen-activated protein (MAP) kinase pathway, while the latter is regulated by the Ras1/cyclic AMP (cAMP) pathway. We previously demonstrated that in the formation of an MTL-homozygous, mature (48 h) sexual biofilm in RPMI 1640 medium, the MAP kinase pathway targets Tec1 rather than Cph1, the latter of which is the target of the same pathway, but for the opaque cell mating response. Here we continued our analysis of the role of Tec1 by comparing the effects of deleting TEC1 on initial adhesion to silicone elastomer, high-resolution confocal microscopy assessments of the stages and cellular phenotypes during the 48 h of biofilm development, human white cell penetration, and biofilm fragility. We show that although Tec1 plays only a minor role in initial adhesion to the silicone elastomer, it does play a major role in the growth of the basal yeast cell polylayer, vertical extension of hyphae and matrix deposition in the upper portion of the biofilm, final biofilm thickness, penetrability of human white blood cells, and final biofilm integrity (i.e., resistance to fluid flow). These results provide a more detailed description of normal biofilm development and architecture and confirm the central role played by the transcription factor Tec1 in the biofilm model employed here.

Original languageEnglish
Pages (from-to)228-240
Number of pages13
JournalEukaryotic Cell
Volume14
Issue number3
DOIs
StatePublished - 1 Jan 2015

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Biofilms
Candida albicans
Silicone Elastomers
Mitogen-Activated Protein Kinases
Hyphae
Human Development
Confocal Microscopy
Cyclic AMP
Leukocytes
Transcription Factors
Yeasts
Phenotype

Cite this

Daniels, Karla J. ; Thyagarajan, Srikantha ; Pujol, Claude ; Park, Yang Nim ; Soll, David R. / Role of Tec1 in the development, architecture, and integrity of sexual biofilms of Candida albicans. In: Eukaryotic Cell. 2015 ; Vol. 14, No. 3. pp. 228-240.
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Role of Tec1 in the development, architecture, and integrity of sexual biofilms of Candida albicans. / Daniels, Karla J.; Thyagarajan, Srikantha; Pujol, Claude; Park, Yang Nim; Soll, David R.

In: Eukaryotic Cell, Vol. 14, No. 3, 01.01.2015, p. 228-240.

Research output: Contribution to journalArticleResearchpeer-review

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