TY - JOUR
T1 - Role of sodium on H+ excretion in the integument of the leopard frog Rana pipiens
AU - Page, Ray
AU - Frazier, Loy W.
AU - Yorio, Thomas
N1 - Funding Information:
A~,know/edgemenrs-This research was supported in part from a grant from the American Heart Association, Texas Affiliate (TY) and a BRSG grant from TCOM (TY). Ray Page was supported by an AOA/Burroughs Wellcome Osteooathic Research Fellowship . (F87-07). The authors would like to thank Drs L. X. Oakford and A. J. Mia for the assistance with quantification of the MR cells by light microscopy.
PY - 1988
Y1 - 1988
N2 - 1. 1. The northern leopard frog, Rana pipiens, pipiens, in contrast to the southern leopard frog, Rana pipiens, berlandieri, did not demonstrate any significant H+ excretion across its integument even during a challenge of chronic metabolic acidosis. Likewise, no increase in the number of H+ secreting mitochondria-rich cells were observed in the northern frogs. 2. 2. Under normal acid-base conditions in the southern frogs, H+ excretion was found to be dependent on mucosal sodium concentrations, whereas during chronic metabolic acidosis, H+ excretion was independent of mucosal sodium concentrations, but was amiloride sensitive. 3. 3. High salinity adapted southern frogs, under normal and acidotic conditions, had enhanced H4+ excretion rates as compared to the control non-salt adapted frogs. 4. 4. Blood analyses demonstrated that significant acid-base changes were the result of systemic acidosis and not due to salt adaptations. Blood Na+ and K+ concentrations were also efficiently maintained during salt adaptations or chronic metabolic acidosis. 5. 5. The results suggest that H+ excretion in epithelia can be influenced by the sodium transport state of the cell and the systemic acid-base profile. Models are proposed explaining these relationships.
AB - 1. 1. The northern leopard frog, Rana pipiens, pipiens, in contrast to the southern leopard frog, Rana pipiens, berlandieri, did not demonstrate any significant H+ excretion across its integument even during a challenge of chronic metabolic acidosis. Likewise, no increase in the number of H+ secreting mitochondria-rich cells were observed in the northern frogs. 2. 2. Under normal acid-base conditions in the southern frogs, H+ excretion was found to be dependent on mucosal sodium concentrations, whereas during chronic metabolic acidosis, H+ excretion was independent of mucosal sodium concentrations, but was amiloride sensitive. 3. 3. High salinity adapted southern frogs, under normal and acidotic conditions, had enhanced H4+ excretion rates as compared to the control non-salt adapted frogs. 4. 4. Blood analyses demonstrated that significant acid-base changes were the result of systemic acidosis and not due to salt adaptations. Blood Na+ and K+ concentrations were also efficiently maintained during salt adaptations or chronic metabolic acidosis. 5. 5. The results suggest that H+ excretion in epithelia can be influenced by the sodium transport state of the cell and the systemic acid-base profile. Models are proposed explaining these relationships.
UR - http://www.scopus.com/inward/record.url?scp=0023677090&partnerID=8YFLogxK
U2 - 10.1016/0300-9629(88)91591-5
DO - 10.1016/0300-9629(88)91591-5
M3 - Article
C2 - 2904333
AN - SCOPUS:0023677090
SN - 0300-9629
VL - 91
SP - 53
EP - 59
JO - Comparative Biochemistry and Physiology -- Part A: Physiology
JF - Comparative Biochemistry and Physiology -- Part A: Physiology
IS - 1
ER -