Rhodopseudomonas palustris CGA010 proteome implicates extracytoplasmic function sigma factor in stress response

Michael S. Allen, Gregory B. Hurst, Tse Yuan S. Lu, Leslie M. Perry, Chongle Pan, Patricia K. Lankford, Dale A. Pelletier

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Rhodopseudomonas palustris encodes 16 extracytoplasmic function (ECF) σ factors. To begin to investigate the regulatory network of one of these ECF σ factors, the whole proteome of R. palustris CGA010 was quantitatively analyzed by tandem mass spectrometry from cultures episomally expressing the ECF σRPA4225 (ecfT) versus a WT control. Among the proteins with the greatest increase in abundance were catalase KatE, trehalose synthase, a DPS-like protein, and several regulatory proteins. Alignment of the cognate promoter regions driving expression of several upregulated proteins suggested a conserved binding motif in the -35 and -10 regions with the consensus sequence GGAAC-18N-TT. Additionally, the putative anti-σ factor RPA4224, whose gene is contained in the same predicted operon as RPA4225, was identified as interacting directly with the predicted response regulator RPA4223 by mass spectrometry of affinity-isolated protein complexes. Furthermore, another gene (RPA4226) coding for a protein that contains a cytoplasmic histidine kinase domain is located immediately upstream of RPA4225. The genomic organization of orthologs for these four genes is conserved in several other strains of R. palustris as well as in closely related α-Proteobacteria. Taken together, these data suggest that ECF σRPA4225 and the three additional genes make up a sigma factor mimicry system in R. palustris.

Original languageEnglish
Pages (from-to)2158-2168
Number of pages11
JournalJournal of Proteome Research
Volume14
Issue number5
DOIs
StatePublished - 1 May 2015

Keywords

  • ECF σ factor
  • Rhodopseudomonas palustris
  • gene regulation
  • proteomics
  • stress

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