RGS14 is a mitotic spindle protein essential from the first division of the mammalian zygote

Luke Martin-McCaffrey; Francis S. Willard; Antonio J. Oliveira-dos-Santos; David R.C. Natale; Bryan E. Snow; Randall J. Kimple; Agnieszka Pajak; Andrew J. Watson; Lina Dagnino; Josef M. Penninger; David P. Siderovski; Sudhir J.A. D'Souza

doi:10.1016/j.devcel.2004.10.004

RGS14 is a mitotic spindle protein essential from the first division of the mammalian zygote

Luke Martin-McCaffrey, Francis S. Willard, Antonio J. Oliveira-dos-Santos, David R.C. Natale, Bryan E. Snow, Randall J. Kimple, Agnieszka Pajak, Andrew J. Watson, Lina Dagnino, Josef M. Penninger, David P. Siderovski, Sudhir J.A. D'Souza

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

Heterotrimeric G protein α subunits, RGS proteins, and GoLoco motif proteins have been recently implicated in the control of mitotic spindle dynamics in C. elegans and D. melanogaster. Here we show that "regulator of G protein signaling-14" (RGS14) is expressed by the mouse embryonic genome immediately prior to the first mitosis, where it colocalizes with the anastral mitotic apparatus of the mouse zygote. Loss of Rgs14 expression in the mouse zygote results in cytofragmentation and failure to progress to the 2-cell stage. RGS14 is found in all tissues and segregates to the nucleus in interphase and to the mitotic spindle and centrioles during mitosis. Alteration of RGS14 levels in exponentially proliferating cells leads to cell growth arrest. Our results indicate that RGS14 is one of the earliest essential product of the mammalian embryonic genome yet described and has a general role in mitosis.

Original language	English
Pages (from-to)	763-769
Number of pages	7
Journal	Developmental Cell
Volume	7
Issue number	5
DOIs	https://doi.org/10.1016/j.devcel.2004.10.004
State	Published - Nov 2004

Access to Document

10.1016/j.devcel.2004.10.004

Cite this

Martin-McCaffrey, L., Willard, F. S., Oliveira-dos-Santos, A. J., Natale, D. R. C., Snow, B. E., Kimple, R. J., Pajak, A., Watson, A. J., Dagnino, L., Penninger, J. M., Siderovski, D. P., & D'Souza, S. J. A. (2004). RGS14 is a mitotic spindle protein essential from the first division of the mammalian zygote. Developmental Cell, 7(5), 763-769. https://doi.org/10.1016/j.devcel.2004.10.004

@article{6053cd258202430fbba580fd06be3dca,

title = "RGS14 is a mitotic spindle protein essential from the first division of the mammalian zygote",

abstract = "Heterotrimeric G protein α subunits, RGS proteins, and GoLoco motif proteins have been recently implicated in the control of mitotic spindle dynamics in C. elegans and D. melanogaster. Here we show that {"}regulator of G protein signaling-14{"} (RGS14) is expressed by the mouse embryonic genome immediately prior to the first mitosis, where it colocalizes with the anastral mitotic apparatus of the mouse zygote. Loss of Rgs14 expression in the mouse zygote results in cytofragmentation and failure to progress to the 2-cell stage. RGS14 is found in all tissues and segregates to the nucleus in interphase and to the mitotic spindle and centrioles during mitosis. Alteration of RGS14 levels in exponentially proliferating cells leads to cell growth arrest. Our results indicate that RGS14 is one of the earliest essential product of the mammalian embryonic genome yet described and has a general role in mitosis.",

author = "Luke Martin-McCaffrey and Willard, {Francis S.} and Oliveira-dos-Santos, {Antonio J.} and Natale, {David R.C.} and Snow, {Bryan E.} and Kimple, {Randall J.} and Agnieszka Pajak and Watson, {Andrew J.} and Lina Dagnino and Penninger, {Josef M.} and Siderovski, {David P.} and D'Souza, {Sudhir J.A.}",

note = "Funding Information: We thank Dr. P. Hunt, B. Wolfe, and M. Welsh for assistance and Drs. G.M. Kidder and C. McCudden for critical appraisal of the manuscript. The Heart and Stroke Foundation of Ontario (T5051 to S.J.A.D.) and NIH GM062338 (to D.P.S.) provided support for the work. L.M.-M. and D.R.C.N. both acknowledge OGS studentships. F.S.W. is a postdoctoral fellow of the American Heart Association. R.J.K. acknowledges predoctoral fellowship support from the NIMH F30 MH64319. S.J.A.D. is a Canadian Institutes of Health Research New Investigator. J.M.P. is supported by IMBA, the Austrian Academy of Sciences, and a grant from the Austrian National Bank. ",

year = "2004",

month = nov,

doi = "10.1016/j.devcel.2004.10.004",

language = "English",

volume = "7",

pages = "763--769",

journal = "Developmental Cell",

issn = "1534-5807",

publisher = "Cell Press",

number = "5",

}

TY - JOUR

T1 - RGS14 is a mitotic spindle protein essential from the first division of the mammalian zygote

AU - Martin-McCaffrey, Luke

AU - Willard, Francis S.

AU - Oliveira-dos-Santos, Antonio J.

AU - Natale, David R.C.

AU - Snow, Bryan E.

AU - Kimple, Randall J.

AU - Pajak, Agnieszka

AU - Watson, Andrew J.

AU - Dagnino, Lina

AU - Penninger, Josef M.

AU - Siderovski, David P.

AU - D'Souza, Sudhir J.A.

N1 - Funding Information: We thank Dr. P. Hunt, B. Wolfe, and M. Welsh for assistance and Drs. G.M. Kidder and C. McCudden for critical appraisal of the manuscript. The Heart and Stroke Foundation of Ontario (T5051 to S.J.A.D.) and NIH GM062338 (to D.P.S.) provided support for the work. L.M.-M. and D.R.C.N. both acknowledge OGS studentships. F.S.W. is a postdoctoral fellow of the American Heart Association. R.J.K. acknowledges predoctoral fellowship support from the NIMH F30 MH64319. S.J.A.D. is a Canadian Institutes of Health Research New Investigator. J.M.P. is supported by IMBA, the Austrian Academy of Sciences, and a grant from the Austrian National Bank.

PY - 2004/11

Y1 - 2004/11

N2 - Heterotrimeric G protein α subunits, RGS proteins, and GoLoco motif proteins have been recently implicated in the control of mitotic spindle dynamics in C. elegans and D. melanogaster. Here we show that "regulator of G protein signaling-14" (RGS14) is expressed by the mouse embryonic genome immediately prior to the first mitosis, where it colocalizes with the anastral mitotic apparatus of the mouse zygote. Loss of Rgs14 expression in the mouse zygote results in cytofragmentation and failure to progress to the 2-cell stage. RGS14 is found in all tissues and segregates to the nucleus in interphase and to the mitotic spindle and centrioles during mitosis. Alteration of RGS14 levels in exponentially proliferating cells leads to cell growth arrest. Our results indicate that RGS14 is one of the earliest essential product of the mammalian embryonic genome yet described and has a general role in mitosis.

AB - Heterotrimeric G protein α subunits, RGS proteins, and GoLoco motif proteins have been recently implicated in the control of mitotic spindle dynamics in C. elegans and D. melanogaster. Here we show that "regulator of G protein signaling-14" (RGS14) is expressed by the mouse embryonic genome immediately prior to the first mitosis, where it colocalizes with the anastral mitotic apparatus of the mouse zygote. Loss of Rgs14 expression in the mouse zygote results in cytofragmentation and failure to progress to the 2-cell stage. RGS14 is found in all tissues and segregates to the nucleus in interphase and to the mitotic spindle and centrioles during mitosis. Alteration of RGS14 levels in exponentially proliferating cells leads to cell growth arrest. Our results indicate that RGS14 is one of the earliest essential product of the mammalian embryonic genome yet described and has a general role in mitosis.

UR - http://www.scopus.com/inward/record.url?scp=7744244752&partnerID=8YFLogxK

U2 - 10.1016/j.devcel.2004.10.004

DO - 10.1016/j.devcel.2004.10.004

M3 - Article

C2 - 15525537

AN - SCOPUS:7744244752

SN - 1534-5807

VL - 7

SP - 763

EP - 769

JO - Developmental Cell

JF - Developmental Cell

IS - 5

ER -

RGS14 is a mitotic spindle protein essential from the first division of the mammalian zygote

Abstract

Access to Document

Other files and links

Fingerprint

Cite this